中文关键词刺萼龙葵植被恢复细菌群落组成高通量测序PICRUSt分析 英文关键词Solanum rostratumvegetation restorationbacterial community structurehigh-throughput sequencingPICRUSt analysis

作者	单位	E-mail
张瑞海	中国农业科学院农业环境与可持续发展研究所, 北京 100081	ruihai.321@163.com
宋振	中国农业科学院农业环境与可持续发展研究所, 北京 100081
付卫东	中国农业科学院农业环境与可持续发展研究所, 北京 100081
郓玲玲	中国农业科学院农业环境与可持续发展研究所, 北京 100081
高金会	中国农业科学院农业环境与可持续发展研究所, 北京 100081
王然	中国农业科学院农业环境与可持续发展研究所, 北京 100081
王忠辉	中国农业科学院农业环境与可持续发展研究所, 北京 100081
张国良	中国农业科学院农业环境与可持续发展研究所, 北京 100081	zhangguoliang@caas.cn

中文摘要 入侵植物可以改变入侵地土壤微生物群落，从而有助于其入侵，在前期研究中发现，植被恢复措施可有效控制刺萼龙葵（Solanum rostratum）的入侵，但植被恢复前后刺萼龙葵根际土壤细菌群落结构与功能尚未清楚.选取了前期研究中的2个植被组合：沙打旺（Astragalus adsurgens）+披碱草（Elymus dahuricus）+无芒雀麦（Bromus inermis）（T1）；沙打旺+苇状羊茅（Festuca arundinacea）+冰草（Agropyron cristatum）+羊草（Leymus chinensis）（T2），并选取刺萼龙葵（SR）及本地植被（NR）作为对照，采用16S rDNA MiSeq高通量测序技术研究刺萼龙葵入侵及植被恢复后刺萼龙葵根际细菌群落组成，同时采用PICRUSt功能预测分析其功能.结果表明，刺萼龙葵入侵（SR）后Simpson指数和Chao1指数均高于本地植被（NP），但未达到显著水平，而植被恢复（T1和T2）后，Shannon指数和Chao1指数显著降低（P<0.05）.刺萼龙葵（SR）显著降低了变形菌门（Proteobacteria）中的微枝形杆菌属（Microvirga）、斯科曼氏菌属（Skermanella）、鞘氨醇单胞菌属（Sphingomonas）及酸杆菌门（Acidobacteria）的Bryobacter属相对丰度（P<0.05），而植被恢复后，这些菌属丰度也随之上升.RDA分析结果显示，土壤有机质、总氮、总磷、总钾和速效钾是影响细菌群落组成的重要因素.PICRUSt功能预测分析表明，刺萼龙葵入侵显著提高了氨基酸合成（biosynthesis of amino acids）、嘌呤代谢（purine metabolism）、嘧啶代谢（pyrimidine metabolism）、核糖体（ribosome）和氨酰-tRNA合成（aminoacyl-tRNA biosynthesis）等方面的功能，而植被恢复以后其相对丰度显著降低.本文探讨了刺萼龙葵入侵及植被恢复后根际细菌群落和功能，为刺萼龙葵的入侵机制及生态恢复提供理论依据. 英文摘要 Invasive plants can change soil microbial communities and therefore promote invasion. While vegetation restoration has been adopted in certain infested lands to curb the invasion of Solanum rostratum, changes in the composition and function of rhizosphere soil bacterial communities of the species before and after the restoration has not yet been reported. In this study, two vegetation combinations used in previous studies were selected as candidates:Astragalus adsurgens+Elymus dahuricus+Bromus inermis (T1) and A. adsurgens+Festuca arundinacea+Agropyron cristatum+Leymus chinensis (T2). Rhizosphere soil samples were collected from each combination (T1 and T2), a S. rostratum invaded area (SR), and the native plant (NP) control to analyze the bacterial community structure and diversity using 16S rDNA gene sequencing on the Illumina MiSeq platform. PICRUSt was further used to predict the functional abilities of soil bacterial communities. Results of 16S rDNA gene sequencing showed that both the Simpson and Chao1 indices were higher in the SR treatment than in the NP treatment, but neither reached a significant level, although both indices decreased significantly after vegetation restoration (T1 and T2; P<0.05). The relative abundance of Microvirga, Skermanella, and Sphingomonas from phylum Proteobacteria and Bryobacter from the phylum Acidobacteria were significantly lower in the SR treatment (P<0.05) when compared with the NP treatment and higher in restoration treatments (T1 and T2). The RDA analysis showed that soil organic matter (OM), total nitrogen (TN), total phosphorus (TP), total potassium (TK), and available potassium (AK) were important factors affecting the composition of the bacterial community. Based on the PICRUSt analysis of soil bacterial community functions, the relative abundance of gene families related to biosynthesis of amino acids, purine metabolism, pyrimidine metabolism, ribosome, and aminoacyl-tRNA biosynthesis were higher in the rhizosphere samples of the SR treatment than those of the NP treatment and reduced significantly after vegetation restoration (T1 and T2; P<0.05). The structure and function of rhizosphere soil bacterial community of S. rostratum and vegetation restoration were analyzed and provided a theoretical basis for the invasion mechanism and ecological restoration of S. rostratum.

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