中文关键词厌氧氨氧化(ANAMMOX)Ni(Ⅱ)短期影响长期影响动力学特征 英文关键词anaerobic ammonium oxidation (ANAMMOX)Ni(Ⅱ)short-term effectslong-term effectskinetics characteristics

作者	单位	E-mail
孙琪	北京工业大学建筑工程学院市政工程系, 北京 100124	743359969@qq.com
赵白航	北京工业大学建筑工程学院市政工程系, 北京 100124	bhzh@bjut.edu.cn
范飒	北京工业大学建筑工程学院市政工程系, 北京 100124
周邦磊	北京工业大学建筑工程学院市政工程系, 北京 100124
李玉琪	北京工业大学建筑工程学院市政工程系, 北京 100124

中文摘要 厌氧氨氧化（ANAMMOX）工艺因其经济环保等优点而广泛用于处理高氨氮污废水，但该工艺对环境条件十分敏感，尤其对重金属.利用批次实验和连续流实验分别研究了Ni（Ⅱ）对ANAMMOX的短期和长期影响.短期作用下，Ni（Ⅱ）对ANAMMOX存在"低促高抑"作用.与不投加Ni（Ⅱ）对比，投加1 mg·L^-1 Ni（Ⅱ）时ANAMMOX的比厌氧氨氧化活性（SAA）提高了11.14%；投加100 mg·L^-1 Ni（Ⅱ）时SAA降低了49.55%，Ni（Ⅱ）的半抑制浓度IC₅₀为83.86 mg·L^-1.而Ni（Ⅱ）对ANAMMOX的长期抑制阈值为15 mg·L^-1.不投加Ni（Ⅱ）时，用Monod方程模拟ANAMMOX动力学，得到q_max0（以TN/VSS计）和K_S0分别为12.25 mg·（g·h）^-1和405.36 mg·L^-1；投加50 mg·L^-1 Ni（Ⅱ）时，用修正后的Haldane模型模拟ANAMMOX动力学，得到q_max（以TN/VSS计）、K_S和K_i分别为6.78 mg·（g·h）^-1、313.28 mg·L^-1和1.32.Ni（Ⅱ）对ANAMMOX动力学的抑制是反竞争性抑制.另外，Ni（Ⅱ）对ANAMMOX脱氮性能的抑制主要与摄入胞内的Ni（Ⅱ）有关，胞内Ni（Ⅱ）对ANAMMOX的IC_{50（胞内）}（以VSS计）为0.072 mg·g^-1. 英文摘要 Anaerobic ammonium oxidation (ANAMMOX) is widely used for treatment of ammonium-rich wastewater because of its economic and environmental benefits. However, ANAMMOX bacteria are sensitive to environmental conditions, especially to heavy metals. The short-term and long-term effects of Ni(Ⅱ) on ANAMMOX were studied by batch and continuous flow experiments, respectively. Results showed that low concentrations of Ni(Ⅱ) had promoted nitrogen removal by ANAMMOX and high concentrations inhibited ANAMMOX performance during a short-term period. Compared with the specific anaerobic ammonium oxidation activity (SAA) without Ni(Ⅱ) addition, SAA with 1 mg·L^-1 Ni(Ⅱ) addition increased by 11.14% and the SAA with 100 mg·L^-1 Ni(Ⅱ) addition reduced by 49.55%. The IC₅₀ of Ni(Ⅱ) for ANAMMOX was determined to be 83.86 mg·L^-1. In contrast, long-term Ni(Ⅱ) addition significantly suppressed nitrogen removal of ANAMMOX, and the suppression threshold of Ni(Ⅱ) on ANAMMOX was 15 mg·L^-1. The Monod model was applied to simulate the kinetics of ANAMMOX without Ni(Ⅱ) addition. The q_max0(TN/VSS) and K_S0 values were 12.25 mg·(g·h)^-1 and 405.36 mg·L^-1, respectively. The modified Haldane model was suitable to describe the kinetics of ANAMMOX with 50 mg·L^-1 Ni(Ⅱ) addition. The q_max(TN/VSS), K_S, and K_i values were 6.78 mg·(g·h)^-1, 313.2 mg·L^-1, and 1.32, respectively. The inhibition of ANAMMOX by Ni(Ⅱ) is anticompetitive inhibition. In addition, the inhibition of Ni(Ⅱ) on ANAMMOX was mainly related to the content of intracellular Ni(Ⅱ). The IC_{50intracellular Ni(Ⅱ)}(VSS) of intracellular Ni(Ⅱ) was 0.072 mg·g^-1.

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