中文关键词SNAD工艺猪场沼液高通量测序技术定量PCR微生物种群 英文关键词simultaneous partial nitrification, ANAMMOX, and denitrification (SNAD)anaerobic digester liquor of swine wastewater (ADLSW)high-throughput sequencingquantitative PCRbacterial community shifts

作者	单位	E-mail
秦嘉伟	成都信息工程大学资源环境学院, 成都 610225	965687512@qq.com
信欣	成都信息工程大学资源环境学院, 成都 610225 中-塞环境与能源"一带一路"联合实验室, 成都 610225	xx@cuit.edu.cn
鲁航	成都信息工程大学资源环境学院, 成都 610225
张萍萍	成都信息工程大学资源环境学院, 成都 610225
王露蓉	成都信息工程大学资源环境学院, 成都 610225
邹长武	成都信息工程大学资源环境学院, 成都 610225
郭俊元	成都信息工程大学资源环境学院, 成都 610225

中文摘要 为了实现合建式连续流同步部分亚硝化、厌氧氨氧化和反硝化SNAD（simultaneous partial nitrification，ANAMMOX，and denitrification）工艺处理实际猪场沼液，保持温度为（30±1）℃，控制溶解氧（DO）为（0.4±0.1）mg·L^-1，首先通过逐步提高模拟进水氨氮浓度来实现SNAD工艺的启动，然后实现SNAD工艺处理实际猪场沼液的稳定运行.同时，采用高通量测序和实时定量PCR（qPCR）技术对反应器启动前后及沼液替换成功时关键生物种群进行分析.结果表明，150 d左右可实现SNAD工艺的启动，298 d完成实际沼液的替换，其出水（NO₃^--N+NO₂^--N）/ΔNH₄⁺-N小于0.11，对NH₄⁺-N和TN的平均去除率为63.26%和55.71%.高通量测序结果表明，绿弯菌门（Chloroflexi，相对丰度50.78%）、变形菌门（Proteobacteria，13.34%）、浮霉菌门（Planctomycetes，9.26%）是沼液替换成功时污泥中的优势菌门；主要优势脱氮菌属Nitrosomonas的相对丰度由启动前1.55%增加到1.98%；两类具有厌氧氨氧化（ANAMMOX）功能菌Candidatus_Brocadia和Candidatus_Kuenenia的相对丰度分别从启动前0.01%和未检出（<0.01%）增加到4.66%和4.18%；Denitratisoma作为主要的反硝化菌，丰度由启动前未检出（<0.01%）增加到2.06%.qPCR结果表明，与接种污泥相比，沼液替换成功后AOB、ANAMMOX菌和反硝化菌的含量均有明显增加.将SNAD工艺用于实际猪场沼液处理，可实现高效稳定脱氮，节约后续处理成本. 英文摘要 To realize a simultaneous partial nitrification, ANAMMOX (anaerobic ammonium oxidation), and denitrification (SNAD) process treating anaerobic digester liquor of swine wastewater (ADLSW) in a continuous-flow biofilm reactor (CFBR), we first gradually increased the influent ammonium (NH₄⁺-N) concentration, and then enhanced the ADLSW ratio in the influent during operation; dissolved oxygen (DO) was controlled at (0.4±0.1) mg·L^-1 by adjusting the air flow rate, and the temperature was kept at (30±1)℃. Meanwhile, high-throughput sequencing and quantitative PCR (polymerase chain reaction) techniques were used to analyze the bacterial community shifts and the amount of dominant nitrogen removal bacteria. The results demonstrated that a successful start-up of the SNAD process was accomplished in 150 d, and replacement of the actual biogas slurry was completed in 298 d. The effluent (NO₃^--N+NO₂^--N)/ΔNH₄⁺-N value was less than 0.11, and the average removal rates of NH₄⁺-N and TN (total nitrogen) increased to 63.26% and 55.71%, respectively. Moreover, high-throughput sequencing results demonstrated that the dominant microbial populations at phylum level were Chloroflexi (with a relative abundance of 50.78%), Proteobacteria (13.34%), and Planctomycetes (9.26%). The relative abundance of Nitrosomonas increased from 1.55% to 1.98%. In addition, the relative abundance of Candidatus_Brocadia and Candidatus_Kuenenia increased from 0.01% and (<0.01%) to 4.66% and 4.18%, respectively, and the relative abundance of Denitratisoma increased from (<0.01%) to 2.06%. Meanwhile, qPCR analysis showed that the amounts of ammonia-oxidizing bacteria, ANAMMOX, and denitrifying bacteria increased significantly compared with the inoculated sludge. An efficient and stable nitrogen removal rate can be achieved, and the follow-up processing cost can be reduced, by application of the SNAD treatment process for ADLSW.

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