中文关键词锰氨氧化锰还原菌同位素标记高通量测序氮去除 英文关键词Mn-ANAMMOXMn(Ⅳ)-reducing bacteriaisotope labelinghigh-throughput sequencingnitrogen removal

作者	单位	E-mail
陈湜	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023	935438118@qq.com
李正魁	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023	zhkuili@nju.edu.cn
覃云斌	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
丁帮璟	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
陈志浩	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023

中文摘要 二氧化锰介导的厌氧氨氧化（锰氨氧化）是最近发现的一种新型微生物脱氮途径，然而很少有研究报道农田沟道中的锰氨氧化过程和反应过程中主要微生物群落锰还原菌.本研究经过340 d锰还原菌富集培养实验，采用同位素示踪技术和高通量测序技术，证实了锰氨氧化在农田沟道土壤中的存在.结果表明，在锰氨氧化过程中可以观察到氨氮的氧化和MnO₂的还原，以及NO₂^-、NO₃^-、³⁰N₂和Mn²⁺的产生，锰氨氧化平均速率为2.88 mg·（kg·d）^-1，氨氮平均去除率为20%，总氮去除率平均可达15%.另外，高通量测序结果表明，经过340 d富集培养实验，在门水平上锰还原菌丰度从原来的27%增加到了70%，其主要的锰还原菌为不动杆菌（Acinetobacter）和地发菌属（Geothrix），相对丰度分别为26.63%和4.07%.实验结果证实了农田沟道中存在二氧化锰介导的厌氧氨氧化过程，可以认为锰氨氧化是微生物脱氮过程的一条重要路径. 英文摘要 Anaerobic ammonium oxidation mediated by MnO₂ (termed Mn-ANAMMOX) is a newly discovered microbial nitrogen removal pathway. However, few studies have reported on the Mn-ANAMMOX process and related microbial communities in agricultural drainage ditches. In this study, Mn(Ⅳ)-reducing bacteria (MnBR) enrichment cultivation was carried out for 340 days and an isotope tracing technique and high-throughput sequencing technology were used to provide convincing evidence of the occurrence of Mn-ANAMMOX. The results showed that simultaneous NH₄⁺ oxidation and MnO₂ reduction occurred during the reaction, and the production of NO₂^-, NO₃^-, ³⁰N₂, and Mn²⁺ was detected. Additionally, the average Mn-ANAMMOX rate, ammonium removal rate, and total nitrogen removal rate were 2.88 mg·(kg·d)^-1, 20%, and 15%, respectively. Moreover, high-throughput sequencing results showed that after 340 d in the enrichment cultivation experiments, the abundance of MnBR increased from 27% to 70% at the phylum level, and the major genera of MnBR were determined as Acinetobacter and Geothrix, with relative abundances of 26.63% and 4.07%, respectively. Overall, the occurrence of Mn-ANAMMOX was directly proven during the MnBR enrichment cultivation experiments, and it might play an essential role in the pathway of microbial nitrogen removal.

PDF全文下载地址:

https://www.hjkx.ac.cn/hjkx/ch/reader/create_pdf.aspx?file_no=20190653&flag=1&journal_id=hjkx&year_id=2019