删除或更新信息,请邮件至freekaoyan#163.com(#换成@)

蓝藻水华形成过程对氮磷转化功能细菌群的影响

本站小编 Free考研考试/2021-12-31

闁归潧顑嗗┃鈧煫鍥跺亰閳ь剛鍠庨崢銈囨嫻鐟欏嫭鏆堥柛鎰缁变即宕ㄩ敓锟�闁挎稑鐬奸悵娑㈠础鐎圭姴绠柛娆愮墬濠€鎵博濞嗘帞銈柡鍌涚懆琚欓柛妯侯儑缂傚鈧潧妫涢悥婊堟晬閿燂拷
中文关键词蓝藻水华细菌群落结构聚磷菌硝化细菌反硝化细菌 英文关键词cyanobacterial bloomsbacterial community structurephosphate accumulating organismnitrifiersdenitrifiers 中文摘要 为探寻蓝藻水华形成过程中细菌群落结构和氮、磷转化功能菌群的动态变化,利用细菌16S rRNA基因的高通量测序和功能基因的荧光定量PCR(qPCR)方法,分析了在蓝藻水华过程中水体细菌群落组成及功能菌群的变化情况.高通量测序结果证明了蓝藻水华形成过程中细菌群落的多样性降低,细菌群落在水华期和非水华期具有不同的结构.随着水华过程中蓝藻密度的增加,水体中Actinobacteria和Bacteroidetes相对丰度减少,而Firmicutes相对丰度增加;蓝藻水华对聚磷菌(PAOs)具有富集作用,对硝化细菌具有抑制作用,而反硝化细菌的数量在中度水华条件下显著增加.qPCR结果显示,随着蓝藻水华的持续发展,硝化和反硝化功能基因丰度下降甚至消失,表明水体中氮转化过程可能受到抑制,此过程也有利于水华过程中微囊藻快速增殖对氮的需求,对水华蓝藻的生长具有正反馈作用. 英文摘要 In order to reveal the dynamic changes of nitrogen-transforming and phosphorus-accumulating bacteria in the process of cyanobacterial bloom formation, bacterial community structure and functional genes associated with nitrogen cycling were analyzed using high-throughput sequencing and real-time quantitative PCR (qPCR). The high-throughput sequencing results showed that the diversity of bacterial communities decreased during cyanobacterial blooms, and the lacustrine free-living and bloom-attached bacterial communities varied with increased bloom density. With increasing density of cyanobacteria, the relative abundance of Actinobacteria and Bacteroidetes decreased, whereas that of Firmicutes increased. Moreover, the growth of phosphate-accumulating organisms was increased in the phycosphere of cyanobacterial blooms, while that of nitrifiers was inhibited. Denitrifiers increased significantly under moderate blooms. The qPCR data also revealed that the abundance of nitrification and denitrification genes decreased or disappeared with the development of cyanobacterial blooms, indicating that the nitrogen transformation process might be inhibited, which showed a positive feedback of bacterial community to the development of Microcystis blooms by satisfying the needs of the nutrients budget.

PDF全文下载地址:

https://www.hjkx.ac.cn/hjkx/ch/reader/create_pdf.aspx?file_no=20181112&flag=1&journal_id=hjkx&year_id=2018

鐎殿喒鍋撻梺顐g摌IP闁挎稒鐭粩鍓х矓瀹ュ牃鍋撻崘顏嗗煛闁兼澘鍟抽惁澶屾喆閸℃侗鏆ラ梻鍛箣缁屽爼鎯囩€e墎绀夋慨锝呯箲濠€鐗堢▔瀹ュ懎鐓傚☉鎾亾闁告帒妫濋幐锟�
547闁圭鍋撻梻鍕╁灪閻楀酣鎳撻崘顏嗗煛闁兼澘鍟畷锟�1130缂佸绉电€垫氨鈧纰嶉弳鈧柡澶嬪姉濞堟垿宕¢崘褏绋囩紒澶婄Ч閸樸倖绺藉Δ鍜佹毌閹煎瓨鎸堕埀顑挎祰椤锛愰幋顖滅婵炴垹鏁稿ú濠囨嚐鏉堫偒鍤旈柕鍡曡兌缁€趁规惔娑掑亾娴g晫妲堥柛鎺撴偠閳ь兛绶氶崳楣冩懚瀹ュ啠鍋撴担鐑樺€炵€规悶鍎埀顑胯兌椤撴悂鎮堕崱鎰ㄥ亾娴f亽浠﹀ù鍏煎搸閳ь兛娴囬崒銊﹀濮樸儮鍋撴担瑙勬畬闁煎弶褰冪缓楣冩偠閸℃劏鍋撴担鐤幀闁哄倸娲㈤埀顑挎祰婢规捇寮甸妯峰亾娴h鐓€闂傚倽顔婄槐鍫曞箻椤撴壕鍋撴担鍦€婇悗娑宠礋閳ь兛绀佺亸鎵偓娑宠礋閳ь兛娴囬鍝ョ不濡や焦绨氶柕鍡曠瀹稿宕g仦鍌楀亾娴e憡鍕鹃柣鐐叉閳ь兛鐒﹂弬鍌氣柦濮瑰洠鍋撴担鍛婃噸閻庢冻璐熼埀顑挎缂嶅鎳栭懠顒冾潶缂佹冻鎷�28缂侇偉顕ч鐔虹矓閹搭垳绀塚IP濞村吋鑹鹃幉铏媴鎼淬倕娈�128.00闁稿繐鍠涚槐锟�
相关话题/南京大学 环境学院 资源 实验室 基因

闁瑰吋绮庨崒锟�2濞戞挸娲ㄩ~鎺楁嚀閸愵亞鍩¢柤鏉垮暢閻﹀鎮介棃娑氭憤濞戞棑璁g槐娆愶紣濡櫣姘ㄩ柨娑樼焷椤锛愰幋顖滅闁稿繐绉烽崹鍌炴偨閿燂拷
濠㈠爢鍥у姤闁告帒妫涢銏ゆ鐎n喖鍘撮柡鍕靛灣椤戝洦绋夐埀顒€鈻庨檱閳ь剙鍟伴悥娲晬鐏炵瓔鍤犲ù婊冮椤┭勬媴閺囩喓鍙€闁归潧褰炵粭鎾寸▔濮樻剚鍤﹂柟绋挎搐閻i箖寮▎鎰稄闁挎稑鏈崹銊ф媼閸涘﹥绠掔€垫澘鐗嗛ˇ鍧楁偪閹达附锛栭柕鍡曞ree濠㈠綊鈧稒銆冮柛鎺戞椤掔喐绋婇悩鐢电Ч闁兼澘鍟伴悥鍝勄庢潏顐熷亾閺囨氨鐟╁☉鎾翠亢椤曡櫕娼忛崨顓у殼20妤犵偠鎻槐婵嬪箑閼姐倗娉㈠ù婊冩缁夊鈧湱鍋熼弫銈夋儍閸曨剙鐦归悗瑙勭閺嗏偓闁哄鍔栭悡锛勬嫚閵忊剝鐓欐繛澶嬫礀瀵攱寰勫鍕槑闁哄倽顫夌涵鍫曟晬鐏炵偓绠掗梻鍥e亾閻熸洑鑳跺▓鎴︽儑鐎n厾绠栭柡澶涙嫹