中文关键词磺胺类抗性基因I型整合酶基因intI 1饮用水源水分布特征水平基因转移 英文关键词sulfonamides antibiotics resistance genesintegrase gene intI 1drinking water sourcedistribution characteristicshorizontal gene transfer

作者	单位	E-mail
胡亚茹	华东理工大学资源与环境工程学院, 国家环境保护化工过程环境风险评价与控制重点实验室, 上海 200237	hu746459@163.com
姜蕾	上海城市水资源开发利用国家工程中心有限公司, 上海 200082
张天阳	华东理工大学资源与环境工程学院, 国家环境保护化工过程环境风险评价与控制重点实验室, 上海 200237
雷丹丹	华东理工大学资源与环境工程学院, 国家环境保护化工过程环境风险评价与控制重点实验室, 上海 200237
姜巍巍	上海城市水资源开发利用国家工程中心有限公司, 上海 200082
张东	上海城市水资源开发利用国家工程中心有限公司, 上海 200082
林匡飞	华东理工大学资源与环境工程学院, 国家环境保护化工过程环境风险评价与控制重点实验室, 上海 200237
崔长征	华东理工大学资源与环境工程学院, 国家环境保护化工过程环境风险评价与控制重点实验室, 上海 200237	cuichangzheng@ecust.edu.cn

中文摘要 饮用水源中检测到的抗生素抗性基因（antibiotic resistance genes，ARGs）对饮用水质安全和人体健康产生的潜在威胁受到广泛关注.在掌握了华东地区某饮用水源地13种磺胺类抗生素的污染特征基础上，进一步采用定性PCR和荧光定量PCR解析该饮用水源水和底泥中磺胺类ARGs（sul 1、sul 2 ）以及抗性基因可转移元件Ⅰ型整合酶基因（intI 1 ）的分布特征.结果表明，3种基因在该饮用水源水和底泥中均100%检出，sul 1 基因是该饮用水源地中检出含量最高的磺胺类ARGs，在水源水中含量范围为1.5×10⁴~6.4×10⁵ copies·mL^-1，底泥中则高达1.6×10⁸ copies·g^-1，较sul 2、intI 1 基因分别高0.6~2.2、0.5~1.9个数量级.sul 1、sul 2 和intI 1 基因在该水源地入水口和出水口处的绝对含量无显著差别，而在底泥中sul 1、sul 2 和intI 1 基因的绝对含量则是出水口高于入水口.sul 1 在夏季水源地出水口的检出含量最高，为6.4×10⁵ copies·mL^-1；intI 1 基因在冬季的检出含量高于其他季节.sul 1 基因与13种磺胺类抗生素具有相关性（r=0.69，P<0.05），其中与磺胺甲唑的含量显著相关（r=0.79，P<0.01）；intI 1 与sul 1、sul 2 的相对含量之间也存在正相关关系（r为0.80和0.73，P<0.05），这表明intI 1 在磺胺类ARGs的水平转移过程中起到了重要作用.本研究为典型饮用水源地中ARGs的污染现状提供基础数据，也为管控饮用水环境的抗性基因污染和制定管理决策提供依据. 英文摘要 Antibiotic resistance genes (ARGs) in drinking water sources have attracted widespread attention due to the threat they pose to water security and human health. This study mainly focused on the distribution of sulfonamide ARGs (sul 1, sul 2) and one integrase gene (intI 1) in water and sediment using qualitative and fluorescent quantitative PCR, based on previous work on the characteristics of 13 kinds of sulfonamides in a drinking water source in East China. Results showed that the three target genes were all detected in water and sediment. The sul 1 gene was the sulfonamide ARG with highest concentration, with 1.5×10⁴-6.4×10⁵ copies·mL^-1 in source water and maximum concentration of 1.6×10⁸ copies·g^-1 in sediment. Concentration of sul 1 was 0.6-2.2, 0.5-1.9 order of magnitudes higher than sul 2 and intI 1 genes, respectively. There was no significant difference between the absolute concentrations of sul 1, sul 2, and intI 1 in inflow and outflow. However, in the case of sediment, absolute abundances of sul 1, sul 2, and intI 1 in outflow were higher than those in inflow. The maximum concentration of sul 1 was detected in outflow in summer (6.4×10⁵ copies·mL^-1). The concentration of intI 1 was higher in winter compared to other seasons. There was a positive correlation between sul 1 and 13 sulfonamides (r=0.69, P<0.05), and the relative concentration of sul 1 and amount of sulfamethoxazole were significantly positively related (r=0.79, P<0.01). There were also positive correlations between the relative concentrations of intI 1 and sul 1, sul 2 (r:0.80 and 0.73, P<0.05), respectively, suggesting that intI 1 played an important role in horizontal gene transfer of sulfonamide ARGs in this drinking water source. This study provides basic data for monitoring pollution of ARGs, as well as a basis for controlling ARG pollution in the drinking water environment and making management decisions.

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