中文关键词东太湖水产养殖沉积物氨氧化细菌氨氧化古菌 英文关键词Eastern Lake Taihuaquaculturesedimentammonia-oxidizing bacteriaammonia-oxidizing archaea

作者	单位	E-mail
储瑜	河海大学水文水资源与水利工程科学国家重点实验室, 南京 210098 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008	heavenbettycy@163.com
何肖微	河海大学水文水资源与水利工程科学国家重点实验室, 南京 210098 中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008
曾巾	中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008
赵大勇	河海大学水文水资源与水利工程科学国家重点实验室, 南京 210098	dyzhao@hhu.edu.cn
孙强	江苏省太湖渔业管理委员会办公室, 苏州 215004
曹萍	江苏省太湖渔业管理委员会办公室, 苏州 215004
吴庆龙	中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008

中文摘要 本研究旨在了解东太湖水产养殖区表层沉积物中氨氧化微生物的群落特征.以编码氨单加氧酶的α亚基（amoA）基因为标记，通过实时荧光定量PCR技术（real-time qPCR）分析环境中好氧氨氧化原核生物的丰度；通过构建克隆文库、测序，进而划分操作分类单元（OTUs）构建系统发育树，分析氨氧化微生物的群落结构与多样性.分析养殖区和对照区表层沉积物中氨氧化微生物群落结构和多样性发现，养殖区中氨氧化细菌（ammonia-oxidizing bacteria，AOB）的丰度更高而对照区中氨氧化古菌（ammonia-oxidizing archaea，AOA）的丰度更高；AOA在养殖区多样性更高，AOB在对照区多样性更高；AOA在养殖区和对照区沉积物中的优势类群均为Nitrosopumilus，AOB在养殖区和对照区沉积物中的优势类群相同均为亚硝化螺旋菌属（Nitrosospira）.水产养殖主要通过影响沉积物中的氨氮含量来影响AOA和AOB的丰度，养殖过程会影响AOA的群落结构而对AOB的群落结构则无明显影响. 英文摘要 The abundance and diversity of ammonia-oxidizing microorganisms in sediments of an aquacultural area of Eastern Lake Taihu were investigated. Real-time quantitative PCR was used to analyze the abundance of archaeal and bacterial amoA genes. Cloned libraries were constructed to investigate the structure and diversity of the microbial community. By comparing community characteristics of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in different zones, we found that:①Copy numbers of the bacterial amoA gene outnumbered those of archaeal amoA genes in the aquacultural zone; ②Diversity of AOA and AOB was higher in the aquaculture zone and control zone, respectively; ③ The dominant cluster of AOA and AOB in both sediments of aquiculture zone and control zone was Nitrosopumilus and Nitrosospira respectively. It was therefore the community structure of AOA (rather than AOB) in lake sediments that was affected by aquacultural activity.

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