中文关键词反硝化厌氧甲烷氧化菌群落多样性时空分布特征环境因子多元直接梯度分析 英文关键词M. oxyferacommunity diversityspatial and temporal distribution characteristicsenvironmental factormultivariate direct gradient analysis

作者	单位	E-mail
张亚迪	中国环境科学研究院城市水环境科技创新基地, 北京 100012 湖南大学环境科学与工程学院, 长沙 410082	18513220869@163.com
宋永会	中国环境科学研究院城市水环境科技创新基地, 北京 100012
彭剑峰	中国环境科学研究院城市水环境科技创新基地, 北京 100012	pjf1995@163.com
张剑桥	深圳市罗湖区环境保护与水务局, 深圳 518000
张盼月	湖南大学环境科学与工程学院, 长沙 410082
刘瑞霞	中国环境科学研究院城市水环境科技创新基地, 北京 100012
石国强	北京清控人居环境研究院有限公司, 北京 100000

中文摘要 为了解浑河底泥中反硝化厌氧甲烷氧化菌——Candidatus Methylomirabilis oxyfera（M.oxyfera）群落多样性的时空分布特征，通过克隆文库技术考察M.oxyfera的16S rRNA和pmoA功能基因的多样性时空分布特征，并结合多元直接梯度分析来研究环境因子（水样和底泥的理化性质）与其之间的关系.9月浑河上、中、下游底泥样品中M.oxyfera的16S rRNA基因的OTU数在2~5之间，Shannon-Wiener多样性指数变化范围为0.21~1.4，呈现出中游 > 上游 > 下游的分布特征.pmoA功能基因上游的OTU数和Shannon-Wiener多样性指数明显高于中游和下游，上游的Shannon-Wiener多样性指数分别是中游和下游的3.5倍和2.3倍，因此M.oxyfera的群落多样性呈现出明显的地域性分布特征.3号采样点处3月采集样品中M.oxyfera的16S rRNA和pmoA的OTU数目分别是6和5，明显高于9月对应的OTU数目，Shannon-Wiener指数也明显比9月高（1.4>0.68；1.57>0.00），因此M.oxyfera的群落多样性呈现出明显的季节分布特征.多元直接梯度分析结果表明底泥中DOC浓度、底泥电导率、水体中TOC含量和水体中亚硝酸氮浓度是影响M.oxyfera群落多样性的主要环境因子. 英文摘要 16S rRNA and pmoA functional genes were used as biomarker genes to investigate the temporal and spatial distribution of community diversity of Candidatus Methylomirabilis oxyfera (M. oxyfera) in the sediments of the Hunhe River by clone library technology. The dependence relationship between the physicochemical property of water and sediment samples and the diversity characteristics of the M. oxyfera community were analyzed by multivariate direct gradient analysis. Among the examples collected in September 2014, the OTU number of the 16S rRNA gene of M. oxyfera was 2-5, the Shannon-Wiener diversity index was 0.21-1.4, and the distribution characteristics in the middle reaches > upstream > downstream were presented. The OTU number and Shannon-Wiener diversity index of the pmoA functional gene in upstream samples are significantly higher than those in the middle and lower reach samples, and the Shannon-Wiener diversity indices in the upstream samples are 3.5 times and 2.3 times higher than that of the middle and the downstream samples, respectively. The community diversity of M. oxyfera showed a distinct regional distribution. Samples were collected at 3 sampling points in March 2015. The OTU values of 16S rRNA and pmoA for M. oxyfera are 6 and 5 respectively, which were obviously higher than those in September 2014. The Shannon-Wiener index is also higher than that in September 2014 (1.4>0.68; 57>0.00). The community diversity of M. oxyfera showed obvious seasonal distribution characteristics. Multivariate direct gradient analysis results showed that the concentration of DOC in water, sediment conductivity, TOC concentration, and nitrite concentration in the sediment are the main environmental factors affecting the community diversity of M. oxyfera.

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