中文关键词沼液稻田高通量测序微生物多样性 英文关键词biogas slurryrice paddyIllumina sequencing technologymicroorganismdiversity

作者	单位	E-mail
朱金山	长江师范学院三峡库区环境监测与灾害防治工程技术研究中心, 重庆 408100 长江师范学院武陵山绿色发展协同创新中心, 重庆 408100 重庆市农业科学院, 重庆 401329	jszhu@163.com
张慧	重庆市农业科学院, 重庆 401329
马连杰	重庆市农业科学院, 重庆 401329
廖敦秀	重庆市农业科学院, 重庆 401329	xiuchai2006@163.com
杨星勇	重庆师范大学生命科学学院, 重庆 401331
王龙昌	西南大学资源环境学院, 重庆 400715
王定勇	西南大学资源环境学院, 重庆 400715

中文摘要 为了探明不同沼灌年限稻田耕层土壤微生物群落多样性变化，明确稻田耕层土壤微生物群落多样性对不同沼灌年限的响应，本文原位系统采集了不同沼灌年限的稻田土壤，并采用高通量测序技术对不同沼灌年限稻田土壤的微生物群落结构进行了分析.结果表明，随着沼灌年限的增加，土壤pH值逐渐下降，有机质、硝态氮、磷酸盐等养分逐渐累积.沼灌不利于水稻产量的形成.高通量测序技术较全面和准确地反映了不同沼灌年限稻田土壤微生物群落结构在门、纲、目、科、属这5个水平上的分布，在沼灌稻田微生物群落多样性的表征方面具有明显的优势.随着沼灌年限的增加，稻田土壤微生物群落的物种丰富度逐渐降低，微生物群落的多样性也逐渐降低.典型对应分析的结果表明，土壤溶解性有机碳（F=2.67，P=0.09）是影响沼灌稻田土壤微生物群落结构的主要环境因子. 英文摘要 In order to explore the variation in soil microbial community diversity in paddy fields with different irrigation periods, we collected in situ rice field soils during different biogas irrigation periods and analyzed the microbial community structures of these soils by high-throughput sequencing. The results showed that as the biogas irrigation period increased, the soil pH decreased gradually, while organic matter, nitrate nitrogen, phosphate, and other nutrients were accumulated. Years of continued biogas irrigation was not conducive to improving rice yields. The results showed that as the biogas irrigation period increased, the richness in microbial species in paddy soils decreased gradually, and the diversity in the microbial communities was also reduced. Proteobacteria accounts for the largest proportion in rice paddy soil with biogas slurry irrigation. With the increase of biogas irrigation years, the proportion of β-Proteobacteria, Bacteroidia, Bacteroidales, Burkholderiales, Bacteroides, and Thiobacillus increased, while the proportion of Gemmatimonadetes and α-Proteobacteria decreased gradually. Dissolved organic carbon (F=2.67, P=0.09) had the greatest effect on microbial community structures in the studied paddy soils.

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