中文关键词铁氨氧化铁还原菌氮去除三价铁还原同位素标记 英文关键词Feammoxiron reducing bacterianitrogen removalFe³⁺ reductionisotope labeling

作者	单位	E-mail
丁帮璟	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023	807348868@qq.com
李正魁	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023	zhkuili@nju.edu.cn
朱鸿杰	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
陈湜	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
覃云斌	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
杨建华	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
胡优优	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023

中文摘要 厌氧氨氧化耦合三价铁还原（称为铁氨氧化）是最近发现的一种新的氮循环路径.然而，很少有研究报道河岸带的氮素铁氨氧化路径.本研究采用同位素示踪技术和高通量测序技术，证明了铁氨氧化在河岸带表层土壤（0~20 cm）的存在.结果表明，铁氨氧化过程能够在河岸带4个不同土壤层（A：0~5 cm，B：5~10 cm，C：10~15 cm，D：15~20 cm）发生，铁氨氧化的速率范围介于0.25~0.29 mg·（kg·d）^-1之间，其中B土壤层铁氨氧化速率显著高于其它土层（P<0.05）.此外，铁还原菌与铁氨氧化密切相关，地杆菌属（Geobacter）和厌氧黏细菌（Anaeromyxobacter）作为铁还原菌在4个土壤层均被检出.在B土壤层中，铁还原菌（Anaeromyxobacter和Geobacter）的丰度显著高于其它土壤层（P<0.05）.总之，厌氧氨氧化耦合三价铁还原共同发生表明铁氨氧化是河岸带氮素去除的一条重要路径. 英文摘要 Anaerobic ammonium oxidation coupled to iron (Ⅲ) reduction (termed Feammox) is a recently discovered pathway of nitrogen cycling. However, little is known about the pathways of N transformation via the Feammox process in riparian zones. In this study, evidence of Feammox in the riparian zone soil layers (0-20 cm) was demonstrated using the isotope tracing technique and a high-throughput sequencing technology. The results showed that Feammox occurred in the riparian zones in four different soil layers (A:0-5 cm, B:5-10 cm, C:10-15 cm, D:15-20 cm) and the Feammox rates ranged from 0.25 mg·(kg·d)^-1 to 0.29 mg·(kg·d)^-1. In the B soil sample, the Feammox rate was significantly higher than in the other soil samples (P<0.05). In addition, iron reducing bacteria played an essential role in the Feammox process, and Anaeromyxobacter and Geobacter were detected in all the soil samples. In the B soil sample, the abundance of iron reducing bacteria was significantly higher than in the other soil samples (P<0.05). Overall, the co-occurrence of ammonium oxidation and iron reduction suggest that Feammox can play an essential role in the pathway of nitrogen removal in riparian zones.

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