中文关键词异养硝化-好氧反硝化复合菌株SBR反应器高通量测序技术菌群结构 英文关键词heterotrophic nitrification-aerobic denitrificationmixed strainsSBR reactorhigh-throughput sequencing technologymicrobial community component

作者	单位	E-mail
陈海升	暨南大学环境学院, 广州 510630	913421208@qq.com
曹刚	暨南大学环境学院, 广州 510630 广东省污染控制与修复材料工程技术中心, 广州 510630	cao_g@163.com
张迪	暨南大学环境学院, 广州 510630
黄郑郑	暨南大学环境学院, 广州 510630
莫测辉	暨南大学环境学院, 广州 510630 广东省污染控制与修复材料工程技术中心, 广州 510630

中文摘要 为探讨复合菌株强化好氧反硝化的脱氮特性以及揭示菌群结构动态演替与生物反应器运行效率之间的联系，以硝态氮为底物，研究了异养硝化-好氧反硝化复合菌株Delftia sp.YH01+Acidovorax sp.YH02强化好氧SBR的脱氮特性，并借助高通量测序技术对强化过程中的菌群结构进行了分析.结果表明，经复合菌株强化后，反应器对NO₃^--N、TN和COD去除率分别提高了12.1%、9.2%和9.4%；复合菌株YH01+YH02的加入对菌群结构产生了较大的影响，菌群结构在属水平上丰度呈上升趋势，多样性呈下降趋势；PCA主成分析和UPGMA聚类分析大致把反应器运行过程分成4个阶段；Delftia和Acidovorax菌的丰度随着反应器呈上升趋势，复合菌株YH01+YH02在SBR反应器内具有良好的适应能力，对好氧反硝化起着非常重要的作用. 英文摘要 The enhanced aerobic denitrification capability of the mixed strains YH01+YH02 in utilizing potassium nitrate, and the dynamic changes in the microbial community component during the period of operation, were evaluated. The microbial community in different stages of the SBR was analyzed by using high-throughput sequencing technology after inoculation with YH01+YH02. The results showed that the NO₃^--N, TN, and COD removal efficiencies increased by 12.1%, 9.2%, and 9.4%, respectively. The relative abundances of the microbes in the microbial community increased at the genus level, and the diversity in the microbial community decreased after enhancement. Principal component analysis and UPGMA analysis revealed that the period of SBR operation was roughly divined into four phases. The relative abundances of Delftia and Acidovorax increased during the period of operation, and YH01+YH02 exhibited excellent compatibility with the SBR ecosystem and played an important part in aerobic denitrification.

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