中文关键词膨胀污泥氮磷失衡沉降性能脂多糖(LPS)群落结构 英文关键词bulking sludgenitrogen and phosphorus imbalancesettleabilitylipopolysaccharide (LPS)microbial community

作者	单位	E-mail
贺雪濛	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023	Sunnyhxm@126.com
丁丽丽	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023	dinglili@nju.edu.cn
张璐璐	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023
顾卓江	浙江海正药业有限公司, 杭州 311404
任洪强	南京大学环境学院, 污染控制与资源化研究国家重点实验室, 南京 210023	hqren@nju.edu.cn

中文摘要 采用高通量测序技术（16S rRNA）、高效液相色谱（HPLC）等方法，重点比较了不同氮磷失衡条件下膨胀污泥性能及群落结构（含膨胀菌）的变化.结果表明，以膨胀污泥为接种污泥（A/O工艺），经过一段时间培养单独限氮（R_N）组的沉降性能可恢复到SVI<150 mL·g^-1，单独限磷（R_P）组SVI指数呈现相对较弱的改善趋势；正常C/N/P（100：5：1）条件的R₀会导致污泥SVI指数最高（SVI=1496 mL·g^-1），其次为同时限制氮磷组（R_NP）.正常营养条件下膨胀污泥脂多糖相对含量（LPS/MLVSS）与沉降性能SVI的皮尔逊相关性分析结果存在显著负相关（r=-0.625，P<0.05）；营养限制条件下污泥（膨胀期）LPS对生物量的指示性更为准确.Thiothrix为4组反应器中主导膨胀菌，PCoA的结果显示Ⅱ、Ⅲ阶段中存在氮限制的组（R_NP、R_N）群落迁移变化较大，RDA结果显示Thiothrix与污泥沉降性能及比耗氧速率呈现显著相关. 英文摘要 In this paper, the performance, characteristics of the bulking sludge, and the variations in the microbial community (including the bulking bacteria) under different nitrogen and phosphorus imbalances were compared, using high-throughput sequencing (16S rRNA) and the high performance liquid chromatography (HPLC) technology. The results showed that after seeding bulking sludge in the A/O process and operating for a period of time, the sludge settleability of the nitrogen limitation alone reactor (R_N) could recover to normal[sludge volume index (SVI)<150 mL·g^-1], while the SVI of the phosphorus limitation alone reactor (R_P) improved slightly; the control reactor (R₀, C/N/P=100/5/1) exhibited the highest SVI index (SVI=1496 mL·g^-1), followed by the reactor of simultaneous nitrogen and phosphorus limitations (R_NP). Under normal nutritional conditions, Pearson correlation analysis showed a significant negative correlation between the lipopolysaccharide (LPS) relative content (LPS/MLVSS) and the settleability of bulking sludge (r=-0.625, P<0.05), while under nutrient limitation conditions, LPS showed high accuracy in reflecting the biomass of the activated sludge. Thiothrix was the dominant bulking bacteria in all the reactors. PCoA analysis showed that the migration of the community in the reactors experienced nitrogen limitation (R_NP, R_N) changes greatly during the stages Ⅱ and Ⅲ, while RDA analysis showed that the correlation of Thiothrix with the settling performance and oxygen consumption rate was significant.

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