摘要:植物内生菌广泛分布于植物的各种组织及器官中，对植物的生长表现出各种作用，而植物种子中的内生菌对植物的作用也越来越受到人们的关注。以榆中县和秦安县两种不同生境的中国沙棘种子为材料，分析中国沙棘种子内生细菌多样性，以期探究生境对种子内生菌多样性的影响，并为进一步研究种子内生菌与沙棘的相互作用提供依据。研究利用纯培养方法和高通量测序方法分别进行中国沙棘种子内生细菌多样性分析。对纯培养分离得到的内生细菌，利用16S rRNA基因序列分析法结合形态学特征进行内生细菌的鉴定；对高通量测序得到的数据进行基于OTUs（Operational Taxonomic units，可操作分类单元）的物种注释分析。通过纯培养方法从榆中县中国沙棘种子中分离得到4株内生细菌，分属于芽孢杆菌属（Bacillus）、葡萄球菌属（Staphylococcus）和假单胞菌属（Pseudomonas）；秦安县中国沙棘种子中分离得到5株内生细菌，分属于芽孢杆菌属（Bacillus）、葡萄球菌属（Staphylococcus）和不动杆菌属（Acinetobacter）。采用高通量测序方法检测到榆中县中国沙棘种子内生细菌分属于7个门、68个属，秦安县中国沙棘种子内生细菌分属于5个门、30个属。在门分类水平，榆中县中国沙棘种子内生细菌的主要优势门类是蓝藻门（Cyanobacteria）和变形菌门（Proteobacteria），相对丰度分别为95.62%和2.03%；秦安县中国沙棘种子的主要优势门类是变形菌门（Proteobacteria）和蓝藻门（Cyanobacteria），相对丰度分别为91.68%和8.06%。在属分类水平，榆中县中国沙棘种子内生细菌的优势菌属为蓝藻细菌属（Cyanobacteria），相对丰度为95.09%；秦安县中国沙棘种子内生细菌的优势菌属为寡养单胞菌属（Stenotrophomonas），相对丰度为85.60%。榆中县和秦安县两种不同生境中国沙棘种子内生细菌有着丰富的多样性，且内生细菌的多样性和丰富度存在明显差异，表现为榆中县中国沙棘种子内生细菌的多样性和丰富度均高于秦安县中国沙棘种子内生细菌。



Abstract:Endophytic bacteria are widely distributed in various tissues and organs of plants and play various roles in the growth of plants. Moreover, the role of endophytic bacteria in plant seeds on plants has attracted more and more attention. In this paper, seeds of Hippophae rhamnoides subsp. sinensis in Yuzhong County and Qin'an County were used as materials to analyze its endogenous bacteria diversity, in order to explore the impact of habitat on the endogenous bacteria diversity of Hippophae rhamnoides subsp. sinensis seeds and to provide a basis for further research on the interaction between Hippophae rhamnoides subsp. sinensis and endophytic bacteria. The diversity of endophytic bacteria in Hippophae rhamnoides subsp. sinensis seeds was analyzed by plate culture method and high-throughput sequencing method. The endophytic bacteria isolated from plate culture method were identified by 16S rRNA gene sequence analysis combined with morphological characteristics. For high-throughput sequencing data based on the analysis of Operational Taxonomic units species annotation. Four strains of endophytic bacteria were isolated from the seeds of Hippophae rhamnoides subsp. sinensis in Yuzhong County by plate culture method, which were classified into Bacillus, Staphylococcus and Pseudomonas. Five strains of endophytic bacteria were isolated from the seeds of Hippophae rhamnoides subsp. sinensis in Qin'an County, which were classified into Bacillus, Staphylococcus and Acinetobacter. The endophytic bacteria of Hippophae rhamnoides subsp. sinensis seeds in Yuzhong County belong to 7 phyla and 68 genera and the endophytic bacteria of Hippophae rhamnoides subsp. Sinensis seeds in Qin'an County belonging to 5 phyla and 30 genera were detected by high throughput sequencing. Among them, at the phylological classification level, the main dominant phylum of endophytic bacteria from Hippophae rhamnoides subsp. Sinensis seeds in Yuzhong County were Cyanobacteria and Proteobacteria, with relative abundance of 95.62% and 2.03%, respectively. The main dominant phyla of Hippophae rhamnoides subsp. Sinensis seeds in Qin'an County were Proteobacteria and Cyanobacteria, with relative abundance of 91.68% and 8.06%, respectively. At the level of genus classification, the dominant endophytic of Hippophae rhamnoides subsp. sinensis seeds in Yuzhong County was Cyanobacteria with a relative abundance 95.09%. The dominant endophytic of Hippophae rhamnoides subsp. Sinensis seeds in Qin'an County was Stenotrophomonas with a relative abundance of 85.60%. The endophytic bacteria of Hippophae rhamnoides subsp. Sinensis seeds in Yuzhong County and Qin'an County were rich in diversity. The diversity and richness of endogenous bacteria were obviously different. The diversity and richness of endophytic bacteria in seeds of Hippophae rhamnoides subsp. Sinensis were higher in Yuzhong County than those in Qin'an County.





PDF全文下载地址:

https://www.ecologica.cn/stxb/article/pdf/stxb201906151267