摘要:2017年7-8月和11-12月在贺兰山岩画风景区采集到369份岩羊新鲜粪便样本，用多态性较高的10个微卫星位点进行基因分型，共识别出282只岩羊个体。通过复合扩増和牙釉蛋白基因PCR法共鉴定出152只雄性，130只雌性。微卫星分析得到平均等位基因（Na）为10.1，平均观测杂合度（Ho）为0.7737，平均期望杂合度（He）为0.6512，平均多态信息（PIC）为0.6129。运用Cervus3.0软件进行亲权鉴定，并用Kingroup V2计算个体间的亲缘系数（r），作为亲权鉴定的补充，共鉴定出父-母-子7对，父-子21对，母-子19对。根据亲缘关系对岩羊的婚配制进行推测分析，表明岩羊婚配制应属一雄多雌。



Abstract:We tested for parentage verification and mating systems in Pseudois nayaur in Helan Mountain Rock Painting Scenic area. 369 fresh faecal samples were collected from July to August and November to December 2017. All faecal samples were then tested through genotyping, using 10 microsatellite loci that had relatively high polymorphism. A total of 282 individuals (152 males and 130 females) were identified through compound amplification and amelogenin gene (AMELX & AMELY) PCR. A total of 101 alleles were detected in 10 microsatellite loci and the average number of alleles (Na) was 10.1, while the mean observed and expected heterozygosities were 0.7737 and 0.6512. Furthermore, the mean value of polymorphic information contents (PIC) was 0.6129. Parentage verification was conducted on Cervus 3.0. Kingroup V2 and was used to calculate the coefficient of relationship (r) between individuals as a supplement to parental identification. The results showed that there were 7 parentage groups, 21 father-child groups and 19 mother-child groups. Based on the analysis of these relationships, we deduced that Pseudois nayaur have a polygynous mating system.





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