杨晓波,
王尚,
薛斌,
李辰宇,
赵辰,
张曦,
谌志强,
王景峰,
邱志刚
军事科学院军事医学研究院环境医学与作业医学研究所, 天津 300050
作者简介: 杨雨桐(1995-),女,硕士研究生,研究方向为耐药基因转移,E-mail:yangyutong0223@163.com;sense0011@163.com.
基金项目: 国家自然科学基金面上项目（42177414）；天津市自然科学基金重点项目（17JCZDJC39100）中图分类号: X171.5
Mechanism of Pheromone-responsive Plasmids Mediated Resistance Genes Conjugation Transfer in Enterococcus faecalis
Yang Yutong,Yang Xiaobo,
Wang Shang,
Xue Bin,
Li Chenyu,
Zhao Chen,
Zhang Xi,
Shen Zhiqiang,
Wang Jingfeng,
Qiu Zhigang
Institute of Environmental and Operational Medicine, Academy of Military Medicine, Chinese Academy of Military Science, Tianjin 300050, China
CLC number: X171.5
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摘要:粪肠球菌是一种在自然环境中广泛存在的革兰氏阳性细菌。由于其特殊的耐药机制及高频率的耐药基因转移方式，导致了环境中耐药粪肠球菌的广泛传播，生态安全形势严峻。其中，信息素应答质粒介导的粪肠球菌耐药基因的接合转移是造成粪肠球菌耐药基因快速扩散的重要方式。本文回顾了近些年关于信息素应答质粒接合转移的研究成果，分析总结了接合转移的必要条件、正负调控信息素对接合转移的调节作用，并以携带四环素抗性的质粒pCF10为例简要探讨了接合转移相关基因、蛋白的调控机制，旨在更加全面地揭示粪肠球菌的耐药基因传播机制，为耐药细菌的基因转移机制研究提供参考。
关键词: 细菌耐药性/
耐药基因/
水平转移/
质粒
Abstract:Enterococcus faecalis is a Gram-positive bacterium that is capable of surviving in a broad range of natural environments. The special resistance mechanism and the high frequency of antibiotic resistance gene transfer cause a wide spread of antibiotic resistance Enterococcus faecalis in the environment which makes challenge to the ecological safety. Among the mechanisms of antibiotic resistance transfer, pheromone-responsive plasmid mediated antibiotic resistance genes conjugation transfer is an important way to cause the rapid spread of antibiotic resistance genes in Enterococcus faecalis. This review will highlight recent advancement of pheromone-responsive plasmid conjugative transfer, including the necessary conditions of conjugation, the function of positive and negative regulatory pheromone on conjugation transfer. And the tetracycline-resistance plasmid pCF10 was taken as an example to expore the regulation mechanism of conjugation transfer related genes and proteins. The review is aiming to reveal the mechanism of antibiotic resistance gene transmission in Enterococcus faecalis and provide reference for the gene transfer mechanism of antibiotic resistance bacteria.
Key words:bacterial resistance/
antibiotic resistance genes/
conjugative transfer/
plasmids.
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