陈新¹,
李紫薇²,
覃宗敏¹,
商群¹,
唐敏¹
1. 海南大学材料与化工学院, 海口 570228;
2. 海南大学环境与植物保护学院, 海口 570228

作者简介: 陈新(1973-),男,讲师,研究方向为海洋环境与生态毒理,E-mail:982912387@qq.com.

基金项目: 国家自然科学基金项目(31360105，31660128，31160098)


中图分类号: X171.5

The Effects of Metal Pyrithione on Sperm DNA of Polychaete Hydroides elegans

Chen Xin¹,
Li Ziwei²,
Qin Zongmin¹,
Shang Qun¹,
Tang Min¹
1. College of Material and Chemical Engineering, Hainan University, Haikou 570228, China;
2. College of Environment and Plant Protection, Hainan University, Haikou 570228, China

CLC number: X171.5

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摘要:吡啶硫酮铜(CuPT)和吡啶硫酮锌(ZnPT)在渗出型海洋防污涂料中的应用日益广泛，其生态毒性引起了人们的关注。本文以南海海域常见优势种——华美盘管虫(Hydroides elegans Haswell)为试验动物，采用彗星实验研究了吡啶硫酮金属对华美盘管虫精子细胞DNA的损伤情况。结果显示，低浓度(4 μg·L^-1 CuPT或8 μg·L^-1 ZnPT)处理组的精子细胞，其“彗星”尾长、尾DNA含量及Olive尾矩都显著高于溶剂对照组(P＜0.05)；较高浓度(8 μg·L^-1或16 μg·L^-1 CuPT，16 μg·L^-1或32 μg·L^-1 ZnPT)处理组的精子细胞，其“彗星”尾长和尾矩多数显著高于溶剂对照组(P<0.01)。此外，尾长和Olive尾矩在试验浓度范围内都呈现“效应-浓度”正相关。CuPT为4 μg·L^-1、ZnPT为8 μg·L^-1时，对精子DNA造成轻度损伤；CuPT为8 μg·L^-1、16 μg·L^-1，ZnPT为16 μg·L^-1、32 μg·L^-1时，则达到了中度损伤的程度。可见吡啶硫酮金属具有较明显的海洋生态遗传毒性；另一方面，华美盘管虫精子细胞DNA对吡啶硫酮金属的胁迫呈现出较高的敏感性和效应-浓度相关性，在作为生态遗传毒性的生物指标方面具有潜在优势，进一步的研究将促进其在海洋重金属污染评价中的应用，特别是用于南海海洋环境的早期预警监测。
关键词: 吡啶硫酮金属/
华美盘管虫/
精子/
生态遗传毒性

Abstract:The application of copper pyrithione (CuPT) and zinc pyrithione (ZnPT) are increasingly widespread in exudation antifouling coatings, and their potential ecotoxicity has attracted a plethora of concerns. In this paper, the effects of CuPT and ZnPT on sperm DNA damage of Hydroides elegans were investigated by comet assay. Results indicated that in groups with low concentration of CuPT (4 μg·L^-1) or ZnPT (8 μg·L^-1), the length of comet tail, DNA contents in tail, and comet Olive tail moment were significantly greater than that of the control (P＜0.05), and also those in the higher concentration groups (CuPT at 8 μg·L^-1 or 16 μg·L^-1, ZnPT at 16 μg·L^-1 or 32 μg·L^-1) were significantly greater than that of the control (P<0.01). A positive “concentration-effect” relationship was observed within the concentration range of this experiment. When CuPT and ZnPT were at 4 μg·L^-1 and 8 μg·L^-1, respectively, DNA damage occurred in a slight degree, while at 8 μg·L^-1 and 16 μg·L^-1 CuPT or 16 μg·L^-1 and 32 μg·L^-1 ZnPT, moderate DNA damage was detected, indicating that CuPT and ZnPT could have obvious ecogenotoxicity in marine environment. In addition, the results also illustrated that Hydroides elegans sperm were sensitive to stress caused by CuPT and ZnPT, and therefore such test can be considered as a potential biomarker for ecogenotoxicity assessment of marine pollution caused by heavy metals, especially for early alert in the South China Sea.
Key words:metal pyrithione/
Hydroides elegans/
sperm/
ecogenotoxicity.