蔡富才,
赵欢,
李艳楠,
张文龙,
杨大佐,
周一兵
大连海洋大学 辽宁省海洋生物资源恢复与生境修复重点实验室, 大连 116023

作者简介: 蔡富才(1991-),男,硕士,研究方向为海洋生态毒理学及海洋污染生境修复,E-mail:caifucai520@126.com.

基金项目: 国家自然科学基金资助项目(41306138)；海洋公益性行业专项(201305002，201305043)；辽宁省教育厅一般项目（L201608）


中图分类号: X171.5

Bioavailablility of Pyrene in the Perinereis aibuhitensis

Cai Fucai,
Zhao Huan,
Li Yannan,
Zhang Wenlong,
Yang Dazuo,
Zhou Yibing
Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning Province, Dalian Ocean University, Dalian 116023, China

CLC number: X171.5

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摘要:为近海岸沉积质中PAHs的生态风险评价及其污染生境的修复提供理论依据,以双齿围沙蚕为研究对象,以芘为暴露污染物,开展双齿围沙蚕对芘的生物可利用性研究,分别考察了芘对双齿围沙蚕抗氧化酶活性的影响以及芘在双齿围沙蚕体内的生物富集特征。结果表明,双齿围沙蚕SOD、CAT和GSH-PX活性均随诱导时间的增加而升高,且SOD活性随芘浓度的增加出现显著性抑制,CAT和GSH-PX活性则随芘浓度的增加出现显著性升高。芘在双齿围沙蚕体内的生物富集特征如下:双齿围沙蚕体内芘的蓄积量随暴露浓度的增加而增加,各处理组双齿围沙蚕体内芘的蓄积量随时间的变化均呈类凸形的二次曲线关系,在第10天达到蓄积的最大值,最大蓄积量依次为(6.18±0.62) mg·kg^-1、(12.37±1.23) mg·kg^-1和(24.76±1.51) mg·kg^-1。生物富集系数BCF随芘浓度的增加而减小,分别为0.0692、0.0684和0.0674。上述研究结果表明,在一定浓度范围内,双齿围沙蚕抗氧化酶活性对芘的暴露具有很好的指示作用;此外,在短期内,双齿围沙蚕对芘具有明显的富集效应。
关键词: 芘/
双齿围沙蚕/
生物可利用性/
抗氧化酶/
生物富集

Abstract:In this article, the bioavailablility of pyrene in Perinereis aibuhitensis was studied. The antioxidant enzyme activities and the characteristics of bioconcentration of pyrene in P. aibuhitensis were detected. The results showed that the activities of SOD, CAT and GSH-PX in P. aibuhitensis were significantly up-regulated with prolonged exposure to pyrene. The activity of SOD was significantly decreased with increased concentration of pyrene, in contrast, the activities of CAT and GSH-PX were increased. The characteristics of bioconcentration of pyrene in P. aibuhitensis were as follows: the accumulation amount of pyrene in P. aibuhitensis showed a quadratic curve pattern with exposure time, which was increased with increased concentration of pyrene. At the 10th day of exposure to pyrene, the accumulation amount in each treatment group reached the maximum with (6.18±0.62) mg·kg^-1, (12.37±1.23) mg·kg^-1 and (24.76±1.51) mg·kg^-1, respectively. The BCFs in each treatment group was 0.0692, 0.0684 and 0.0674, respectively, which showed negative relationship with pollutant concentration. These results indicated that the activity of antioxidant enzymes in P. aibuhitensis may act as a good indicator for pyrene exposure in a certain concentration range, and furthermore, P. aibuhitensis has the ability to bioaccumulate pyrene. This article will support the further study on ecological risk evaluation and environmental remediation in the coastal sediment polluted by PAHs.
Key words:pyrene/
Perinereis aibuhitensis/
bioavailablility/
antioxidant enzyme/
bioconcentration.