钱伟强 邮  箱：wqqian@pku.edu.cn
职  称：研究员
实验室地址：北京市海淀区颐和园路5号，北京大学，王克桢楼，100871


个人简介
科研领域
代表性论文
教育经历:2011 - 2011 , 博士后 , 遗传学 , 美国普渡大学
2009 - 2010 , 博士后 , 遗传学 , 美国加州大学河滨分校
2007 - 2009 , 博士后 , 遗传学 , 美国康奈尔大学
2002 - 2007 , 理学博士 , 遗传学 , 中国科学院遗传与发育生物学研究所
1998 - 2002 , 理学学士 , 生物学 , 北京师范大学
工作经历:2013 - 至今 , 研究员 , 北京大学生命科学学院
2012 - 2013 , 青年研究员 , 中国科学院上海植物逆境生物学研究中心
　　实验室研究背景和方向： 本实验室以拟南芥为模式系统，研究表观遗传学信息如何影响基因表达以及植物生长发育，主要研究方向为DNA去甲基化途径和转座子对附近基因的表达调控。
　　DNA甲基化修饰是一种重要的表观遗传学标志，在基因的表达调控，基因组的结构及稳定性，X染色体的失活和其它生长发育调控等方面都具有十分重要的作用。DNA甲基化的水平主要由甲基化和去甲基化这两个途经来协同调控。在植物体内，DNA去甲基化主要是由repressor of silencing 1 (ROS1)基因家族介导的碱基切除修复机制来实现。在植物中对DNA甲基化途经的研究已经比较深入，但是对DNA去甲基化的分子机制还不清楚。我们将运用遗传学，生物化学和细胞生物学的方法，对植物体内DNA去甲基化的酶学机制以及调控去甲基化的途经进行深入的研究。
　　　　
1. Li Y*, Córdoba-Ca?ero D*, Qian W*, Zhu X, Tang K, Ariza, R Arjona T and Zhu JK , An AP endonuclease functions in active DNA demethylation and gene imprinting in Arabidopsis , PLoS Genet , 2015 , 11(1): e**. doi:10.1371/journal.pgen.** (*Contributed equally to this work)
2. Duan C, Zhang H, Tang K, Zhu X, Qian W, Hou Y, Wang B, Lang Z, Zhao Y, Wang X, Wang P, Zhou J, Liang G, Liu N, Wang C, Zhu JK , Specific but interdependent functions for Arabidopsis AGO4 and AGO6 in RNA-directed DNA methylation , EMBO J , 2014 , pii: e
3. Qian W*, Miki D*, Lei M*, Zhu X, Zhang H, Liu Y, Li Y, Lang Z, Wang J, Tang K, Liu R and Zhu JK. , Regulation of Active DNA Demethylation by an α-Crystallin Domain Protein in Arabidopsis , Mol Cell , 2014 , 55(3):361-71(*Contributed equally to this work)
4. Zhang H, Tang K, Qian W, Duan CG, Wang B, Zhang H, Wang P, Zhu X, Lang Z, Yang Y and Zhu JK , An Rrp6-like protein positively regulates noncoding RNA levels and DNA methylation in Arabidopsis , Mol Cell , 2014 , 8;54(3):418-30
5. Li X, Qian W, Zhao Y, Wang C, Shen J, Zhu J-K and Gong Z , The anti-Silencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis , Proc Natl Acad Sci U S A , 2012 , 109(28):11425-30
6. Qian W*, Miki D*, Zhang H, Liu Y, Zhang X, Tang K, Kan Y, La H, Li X, Li S, Zhu X, Shi X, Zhang K, Pontes O, Chen X, Liu R, Gong Z and Zhu JK , A Histone Acetyltransferase Regulates Active DNA Demethylation in Arabidopsis , Science , 2012 , 336(6087):1445-8
7. Mang HG*, Qian W*, Zhu Y, Qian J, Kang HG, F.Klessig D and Hua J , ABA deficiency antagonizes high temperature inhibition of disease resistance through enhancing nuclear accumulation of resistance proteins SNC1 and RPS4 in Arabidopsis , Plant Cell , 2012 , 24 (3): 1271-84,*Contributed equally to this work
8. Martínez-Macías M*, Qian W*, Miki D*, Pontes O, Liu Y, Tang K, Liu R, Morales-Ruiz T, R. Ariza R, Roldán-Arjona R, and Zhu JK , A DNA 3’-phosphatase functions in active DNA demethylation in Arabidopsis , Mol Cell , 2012 , 45(3): 357-70,*Contributed equally to this work
9. Wang L*, Li Z*, Qian W, Guo W, Gao X, Huang L, Wang H, Zhu H, Wu JW, Wang D and Liu D , Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and play an important role in plant tolerance to phosphate limitation , Plant Physiol , 2011 , 157 (3): 1283-99
10. Tran HT, Qian W, Hurley BA, She YM, Wang D, and Plaxton WC , Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana , Plant Cell Environ , 2010 , 33 (11): 1789-803
11. Gao Z, Liu HL, Daxinger L, Pontes O, He X, Qian W, Lin H, Xie M, Lorkovic ZJ, Zhang S, Miki D, Zhan X, Pontier D, Lagrange T, Jin H, Matzke AJ, Matzke M, Pikaard CS and Zhu JK , An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation , Nature , 2010 , 6;465(7294):106-9
12. Zhu Y, Qian W, and Hua J , Temperature modulates plant defense responses through NB-LRR proteins , PLoS Pathog , 2010 , 1;6 (4):e**
13. Qin C, Qian W, Wang W, Wu Y, Yu C, Jiang X, Wang D, Wu P , GDP-mannosepyrophosphorylase is a genetic determinant of ammonium sensitivity in Arabidopsis thaliana , Proc Natl Acad Sci USA , 2008 , 25;105(47):18308-13
14. Liu X*，Qian W*, Liu X, Qin H, and Wang D , Molecular and functional analysis of hypoxanthine-guanine phosphoribosyltransferase from Arabidopsis thaliana , New Phytol , 2007 , 175(3):448-61,* Contributed equally to this work
15. Qian W, Yu C, Qin H, Liu X, Zhang A, Johansen, I.E, and Wang D , Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana , Plant J , 2007 , 49(3):399-413