教育经历
工作经历
研究概述
发表文章
何新建 博士
北京生命科学研究所高级研究员
Xinjian He, Ph.D. Associate Investigator, NIBS, Beijing，China
Phone：-8555
Fax：
E-mail：hexinjian@nibs.ac.cn
教育经历
Education
1997
南京师范大学生物系生物学学士学位
B.S. Biology, Department of Biology,
2000
南京农业大学农学系作物遗传育种学硕士学位
M.S. Crop Genetics and Breeding, Department of Agronomy,
2004
中科院遗传与发育生物学研究所生化与分子生物学博士学位
Ph.D. Biochemistry and Molecular Biology, Institute of Genetics and Developmental Biology,
工作经历
Professional Experience
2015-present
北京生命科学研究所高级研究员
Associate Investigator, National Institute of Biological Sciences,Beijing,China
2010-2015
北京生命科学研究所研究员
Assistant Investigator, National Institute of Biological Sciences,Beijing,China
2006-2009
美国加州大学河滨分校植物科学系博士后
Postdoctoral Research Associate, Department of Botany and Plant Sciences,UniversityofCalifornia,Riverside,USA
2004-2006
美国爱荷华大学生物系博士后
Postdoctoral Researcher, Department of Biology,University of Iowa,USA
研究概述
DNA甲基化和组蛋白修饰引起的染色质变化对于基因组稳定性、基因表达调控、基因组印记和转基因沉默具有重要作用。DNA甲基化是指在DNA的胞嘧啶碱基的5号位加上一个甲基，它广泛存在于原核生物和真核生物中。在植物基因组中，甲基化的DNA位点往往伴随产生小分子的siRNA和非编码的长链RNA，这些RNA可以通过RNA指导的DNA甲基化途径引导基因组DNA的甲基化。组蛋白修饰包括甲基化、乙酰化、磷酸化和泛素化等，这些修饰和DNA甲基化协同作用，共同调控染色质的状态。我们将利用模式植物拟南芥为研究材料，通过利用遗传、分子和生化方法重点研究RNA指导的DNA甲基化和组蛋白修饰途径。我们将鉴定这个途径中的新组分，并研究它们在维持基因组稳定性，调控发育和转基因沉默机制中的重要功能。这将有助于我们深入理解植物体在发育和环境适应过程中表观遗传的动态调控机制。
Research Description
Chromatin changes caused by DNA methylation and histone modifications play crucial roles in genome stability, gene regulation, genomic imprinting and transgene silencing. DNA methylation refers to addition of a methyl group to5’-cytosine of DNA, which extensively exists from prokaryotes to eukaryotes. In plants, the methylated genomic DNA loci are frequently accompanied by the occurrence of siRNAs and noncoding RNAs, which cause de novo DNA methylation through RNA-directed DNA methylation. The histone modifications include methylation, acetylation, phosphorylation and ubiquitination, which coordinate with DNA methylation and regulate chromatin status. We will study the RNA-directed DNA methylation and histone modification mechanisms by applying genetic, molecular biological and biochemical approaches. We attempt to identify new components in the pathway and study their functions in genome stability, development and transcriptional gene silencing. These studies will help to understand the dynamic regulation of epigenomes during plant development and adaptation to environments.
发表文章
Publications
1. Liu ZW, Shao CR, Zhang CJ, Zhou JX, Zhang SW, Li L, Chen S, Huang HW, Cai T,He XJ.(2014) The SET Domain Proteins SUVH2 and SUVH9 Are Required for Pol V Occupancyat RNA-Directed DNA Methylation Loci. PLoS Genet. 10, e**.
2. Huang CF, Miki D, Tang K, Zhou HR, Zheng Z, Chen W, Ma ZY, Yang L, Zhang H,Liu R,He XJ, Zhu JK. (2013) A Pre-mRNA-splicing factor is required for RNA-directed DNAmethylation in Arabidopsis. PLoS Genet. 9, e**.
3. Zhou HR, Zhang FF, Ma ZY, Huang HW, Jiang L, Cai T, Zhu JK, Zhang C, He XJ.(2013) Folatepolyglutamylation is involved in chromatin silencing by maintaining globalDNA methylation and histone H3K9 dimethylation in Arabidopsis. Plant Cell25, 2545-2559.
4. Dou K, Huang CF, Ma ZY, Zhang CJ, Zhou JX, Huang HW, Cai T, Tang K, Zhu JK, HeXJ.(2013) The PRP6-like splicing factor STA1 is involved in RNA-directed DNAmethylation by facilitating the production of Pol V-dependent scaffold RNAs.Nucleic Acids Res.41, 8489-8502.
5. Zhang H, Ma ZY, Zeng L, Tanaka K, Zhang CJ, Ma J, Bai G, Wang P, Zhang SW, LiuZW, Cai T, Tang K, Liu R, Shi X, He XJ*, Zhu JK*. (2013) DTF1 is a core component ofRNA-directed DNA methylation and may assist in the recruitment of Pol IV. Proc. Natl. Acad. Sci. USA110, 8290-8295. (*co-corresponding authors).
6. Zhang CJ, Zhou JX, Liu J, Ma ZY, Zhang SW, Dou K, Huang HW, Cai T, Liu R, ZhuJK,He XJ.(2013) The splicing machinery promotes RNA-directed DNA methylation andtranscriptional silencing in Arabidopsis. EMBO J. 32, 1128-1140.
7. Zhang CJ, Ning YQ, Zhang SW, Chen Q, Shao CR, Guo YW, Zhou JX, Li L, Chen S,He XJ.(2012) IDN2 and its paralogs form a complex required for RNA-directed DNAmethylation. PLoS Genet. 8, e**.
8. Liu J, Bai G, Zhang C, Chen W, Zhou J, Zhang S, Chen Q, Deng X, He XJ*, Zhu JK. (2011) An atypical component of RNA-directed DNA methylation machinery has both DNAmethylation-dependent and -independent roles in locus-specific transcriptionalgene silencing. Cell Res. 21, 1691-1700. (*corresponding author).
9. He XJ*, Chen T*, Zhu JK. (2011) Regulation and function of DNA methylation in plantsand animals. Cell Res. 21, 442-465. (*co-corresponding authors).
10. Gao Z, Liu HL, Daxinger L, Pontes O, He XJ, Qian W, Lin H, Xie M, Lorkovic ZJ, Zhang S, Miki D, Zhan X, Pontier D, Lagrange T, Jin H, Matzke AJ, Matzke M,Pikaard CS, Zhu JK. An RNA polymerase II- and AGO4-associated protein acts inRNA-directed DNA methylation. Nature465, 106-109.
11. Zheng Z, Xing Y, He XJ, Li W, Hu Y, Yadav SK, Oh J, Zhu JK. (2010) An SGS3-likeprotein functions in RNA-directed DNA methylation and transcriptional genesilencing in Arabidopsis. Plant J. 62, 92-99.
12. He XJ, Hsu YF, Zhu S, Liu HL, Pontes O, Zhu J, Cui X, Wang CS, Zhu JK. (2009) A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development. Genes Dev. 23, 2717-2722.
13. He XJ, Hsu YF, Zhu S, Wierzbicki AT, Pontes O, Pikaard CS, Liu HL, Wang CS, Jin H, Zhu JK. (2009) An effector of RNA-directed DNA methylation in Arabidopsis is an ARGONAUTE 4- and RNA-binding protein. Cell 137, 498-508.
14. He XJ, Hsu YF, Pontes O, Zhu J, Lu J, Bressan RA, Pikaard CS, Wang CS, Zhu JK. (2009) NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation. Genes Dev. 23, 318-330.
15. He XJ, Mulford KE, Fassler JS. (2009) Oxitative stress function of the S. cerevisiae Skn7 receiver domain. Eukaryot. Cell 8, 768-778.
16. Li WX, Oono Y, Zhu J, He XJ, Wu J, Iida K, Lu XY, Cui X, Jin H, Zhu JK. (2008) The Arabidopsis NFYA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance. The Plant Cell 20, 2238-2251.
17. Cao WH, Liu J, He XJ, Mu RL, Zhou HL, Chen SY, Zhang JS. (2007) Modulation of ethylene responses affects plant salt-stress responses. Plant Physiol. 143, 707-19.
18. He XJ, Fassler JS. (2005) Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae.MolMicrobiol. 58, 1454-1467.
19. He XJ, Mu RL, Cao WH, Zhang ZG, Zhang JS, Chen SY. (2005) AtNAC2, a transcription factor downstream of ethylene and auxin signaling pathways, is involved in salt stress response and lateral root development. Plant J. 44, 903-916.
20. Zhang WK, Shen YG, He XJ, Du BX, Xie ZM, Luo GZ, Zhang JS, Chen SY. (2005) Characterization of a novel cell cycle-related gene from Arabidopsis. J Exp Bot. 56, 807-816.
21. He XJ, Zhang ZG, Yan DQ, Zhang JS, Chen SY. (2004) A salt-responsive receptor-like kinase gene regulated by the ethylene signaling pathway encodes a plasma membrane serine/threonine kinase.TheorAppl Genet. 09, 377-383.
22. Wang YJ, Zhang ZG, He XJ, Zhou HL, Wen YX, Dai JX, Zhang JS, Chen SY. (2003) A rice transcription factor OsbHLH1 is involved in cold stress response. TheorAppl Genet. 107, 1402-1409.
23. Zhang ZG, Gong Y, He XJ, Wang YJ, Sun ZX, Zhang JS, Chen SY. (2003) Cloning of the full length gene for tobacco ethylene receptor NTHK2 and characterization of its kinase domain. ActaBotanicaSinica.45, 68-72.
24. He XJ, Chen J, Zhang Z, Zhang J, Chen S. (2002) Identification of salt-stressresponsive genes in rice (Oryza sativa L.) by cDNA array. Sci China C Life Sci.45, 477-484.
25. Xie C, Zhang ZG, Zhang JS, He XJ, Cao WH, He SJ, Chen SY. (2002) Spatial expression and characterization of a putative ethylene receptor protein NTHK1 in tobacco. Plant Cell Physiol. 43, 810-815.
26. Wang JF, He XJ, Zhang HS, Chen ZY. (2002) Genetic analysis of blast resistance in japonica rice landrace heikezijing from Taihu region. Yi ChuanXueBao. 29, 803-807.
