Yajing Liu
Shisheng Huang
Xingxu Huang
Tian Chi
aSchool of Life Sciences and Technology, ShanghaiTech University, Shanghai, 201210, China
bCAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China
cUniversity of Chinese Academy of Sciences, Beijing, 100049, China
dDepartment of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA
More InformationCorresponding author: E-mail address: chitian@shanghaitech.edu.cn (Tian Chi)
Received Date: 2019-07-18
Accepted Date:2019-11-04
Rev Recd Date:2019-10-08
Available Online: 2019-11-27 Publish Date:2019-11-20
Abstract
Abstract
Site-directed RNA editing (SDRE) is invaluable to basic research and clinical applications?and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lessons from the published studies as well as our ongoing research. Overall, SDRE is still at its infancy, with significant challenges and exciting opportunities ahead.Keywords: Gene editing,
RNA editing,
Cas13,
Off-target,
Specificity
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