Gonghao Jiang
Longwei Yang
Lei Qiu
Ping He
Chunxiao Nong
Yunyue Wang
Yuqing He
Yongzhong Xing
aNational Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
bCollege of Life Science, Heilongjiang University, Harbin 150080, China
cHubei Collaborative Innovation Center for Grain Industry, Jingzhou 434025, China
dKey Laboratory of Plant Pathology, Yunnan Agricultural University, Kunming 650224, China
More InformationCorresponding author: E-mail address: yzxing@mail.hzau.edu.cn (Yongzhong Xing)
Received Date: 2018-04-26
Accepted Date:2018-08-28
Rev Recd Date:2018-07-17
Available Online: 2018-10-22 Publish Date:2018-10-20
Abstract
Abstract
The fungus Magnaporthe oryzae threatens the rice production of Kongyu 131 (KY131), a leading japonica variety in Northeast China. In this study, two rice lines, KP1 and KP2-Hd1, were obtained by introgressing the blast resistance genes Pi1 and Pi2 into KY131, respectively. However, both lines headed later than KY131. RICE60K SNP array analysis showed that Hd1 closely linked to Pi2 was introgressed into KP2-Hd1, and the linkage drag of Hd1 was broken by recombination. On the other hand, no known flowering genes were introgressed into KP1. Gene diagnosis by resequencing six flowering genes showed that KP1 carried functional Hd16 and Ghd8 alleles. Due to its suppression role in heading under long-day conditions, Ghd8 was chosen as the target for gene editing to disrupt its function. Four sgRNAs targeting different sites within Ghd8 were utilized to induce large-deletion mutations, which were easy to detect via agarose gel electrophoresis. All the ghd8-mutated KP1 lines were resistant to rice blast disease and headed earlier than the control KP1, even than KY131, under natural long-day conditions, which ensures its growth in Northeast China. This study confirmed that a combination of gene diagnosis and targeted gene editing is a highly efficient way to quickly eliminate undesired traits in a breeding line.Keywords: Blast resistance,
Heading date,
Gene diagnosis,
Large deletion,
Gene editing
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