A highly efficient in?vivo plasmid editing tool based on CRISPR-Cas12a and phage λ Red recombineerin

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Yiman Geng1, #,
Haiqin Yan2, #,
Pei Li3,
Gaixian Ren3,
Xiaopeng Guo3,
Peiqi Yin3,
Leiliang Zhang3,
Zhaohui Qian3 ,
Zhendong Zhao3 ,
Yi-Cheng Sun3
1NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China
2Department of Basic Medical Sciences, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, 233030, China
3NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Center for Tuberculosis Research, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100730, China

More InformationCorresponding author: E-mail address: zqian2013@sina.com (Zhaohui Qian);E-mail address: timjszzd@163.com (Zhendong Zhao);E-mail address: sunyc@ipbcams.ac.cn (Yi-Cheng Sun)
Received Date: 2019-03-10
Accepted Date:2019-07-04
Rev Recd Date:2019-06-16
Available Online: 2019-09-10 Publish Date:2019-09-20

Abstract

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http://www.jgenetgenomics.org/article/exportPdf?id=f9323f83-96bf-47c5-9e28-032bd2222fe0&language=en

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