Molecular Plant
Abstract
Genome editing provides novel strategies for improving plant traits, but relies on current genetic transformation and plant regeneration procedures, which can be inefficient. We have engineered a barley stripe mosaic virus (BSMV)-based sgRNA delivery vector (BSMV-sg) that is effective in performing heritable genome editing in Cas9-transgenic wheat plants. Mutated progenies were present in the next generation at frequencies ranging from 12.9% to 100% in three different wheat varieties, and 53.8% to 100% of mutants were virus-free. We also achieved multiplex mutagenesis in progeny using a pool of BSMV-sg vectors harboring different sgRNAs. Furthermore, we devised a virus-induced transgene-free editing procedure (VITF-Edit) to generate Cas9-free wheat mutants by crossing BSMV-infected Cas9-transgenic wheat pollen with wild-type wheat. Our study provides a robust, convenient and tissue culture-free approach for genome editing in wheat through virus infection.
| 论文编号: | DOI:10.1016/j.molp.2021.07.010 |
| 论文题目: | Highly Efficient Heritable Genome Editing in Wheat Using an RNA Virus and Bypassing Tissue Culture |
| 英文论文题目: | Highly Efficient Heritable Genome Editing in Wheat Using an RNA Virus and Bypassing Tissue Culture |
| 第一作者: | Tingdong Li, Jiacheng Hu, Yu Sun, Boshu Li, Dingliang Zhang, Wenli Li, Jinxing Liu, Dawei Li, Caixia Gao, Yongliang Zhang, Yanpeng Wang |
| 英文第一作者: | Tingdong Li, Jiacheng Hu, Yu Sun, Boshu Li, Dingliang Zhang, Wenli Li, Jinxing Liu, Dawei Li, Caixia Gao, Yongliang Zhang, Yanpeng Wang |
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| 发表年度: | 2021-07-16 |
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| 摘要: | Genome editing provides novel strategies for improving plant traits, but relies on current genetic transformation and plant regeneration procedures, which can be inefficient. We have engineered a barley stripe mosaic virus (BSMV)-based sgRNA delivery vector (BSMV-sg) that is effective in performing heritable genome editing in Cas9-transgenic wheat plants. Mutated progenies were present in the next generation at frequencies ranging from 12.9% to 100% in three different wheat varieties, and 53.8% to 100% of mutants were virus-free. We also achieved multiplex mutagenesis in progeny using a pool of BSMV-sg vectors harboring different sgRNAs. Furthermore, we devised a virus-induced transgene-free editing procedure (VITF-Edit) to generate Cas9-free wheat mutants by crossing BSMV-infected Cas9-transgenic wheat pollen with wild-type wheat. Our study provides a robust, convenient and tissue culture-free approach for genome editing in wheat through virus infection. |
| 英文摘要: | Genome editing provides novel strategies for improving plant traits, but relies on current genetic transformation and plant regeneration procedures, which can be inefficient. We have engineered a barley stripe mosaic virus (BSMV)-based sgRNA delivery vector (BSMV-sg) that is effective in performing heritable genome editing in Cas9-transgenic wheat plants. Mutated progenies were present in the next generation at frequencies ranging from 12.9% to 100% in three different wheat varieties, and 53.8% to 100% of mutants were virus-free. We also achieved multiplex mutagenesis in progeny using a pool of BSMV-sg vectors harboring different sgRNAs. Furthermore, we devised a virus-induced transgene-free editing procedure (VITF-Edit) to generate Cas9-free wheat mutants by crossing BSMV-infected Cas9-transgenic wheat pollen with wild-type wheat. Our study provides a robust, convenient and tissue culture-free approach for genome editing in wheat through virus infection. |
| 刊物名称: | Molecular Plant |
| 英文刊物名称: | Molecular Plant |
| 论文全文: | Genome editing provides novel strategies for improving plant traits, but relies on current genetic transformation and plant regeneration procedures, which can be inefficient. We have engineered a barley stripe mosaic virus (BSMV)-based sgRNA delivery vector (BSMV-sg) that is effective in performing heritable genome editing in Cas9-transgenic wheat plants. Mutated progenies were present in the next generation at frequencies ranging from 12.9% to 100% in three different wheat varieties, and 53.8% to 100% of mutants were virus-free. We also achieved multiplex mutagenesis in progeny using a pool of BSMV-sg vectors harboring different sgRNAs. Furthermore, we devised a virus-induced transgene-free editing procedure (VITF-Edit) to generate Cas9-free wheat mutants by crossing BSMV-infected Cas9-transgenic wheat pollen with wild-type wheat. Our study provides a robust, convenient and tissue culture-free approach for genome editing in wheat through virus infection. |
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| 其它备注: | Tingdong Li, Jiacheng Hu, Yu Sun, Boshu Li, Dingliang Zhang, Wenli Li, Jinxing Liu, Dawei Li, Caixia Gao, Yongliang Zhang, Yanpeng Wang. Highly Efficient Heritable Genome Editing in Wheat Using an RNA Virus and Bypassing Tissue Culture. Molecular Plant. DOI:10.1016/j.molp.2021.07.010 |
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