Ming-Hong He
Jia-Cheng Liu
Yi-Si Lu
Jing Peng
Jin-Qiu Zhou
aCAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China
bSchool of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
More InformationCorresponding author: E-mail address: jqzhou@sibcb.ac.cn (Jin-Qiu Zhou)
Received Date: 2018-01-30
Accepted Date:2018-03-23
Rev Recd Date:2018-03-15
Available Online: 2018-05-04 Publish Date:2018-05-20
Abstract
Abstract
In Saccharomyces cerevisiae, the highly conserved Sua5 and KEOPS complex (including five subunits Kae1, Bud32, Cgi121, Pcc1 and Gon7) catalyze a universal tRNA modification, namely N6-threonylcarbamoyladenosine (t6A), and regulate telomere replication and recombination. However, whether telomere regulation function of Sua5 and KEOPS complex depends on the t6A modification activity remains unclear. Here we show that Sua5 and KEOPS regulate telomere length in the same genetic pathway. Interestingly, the telomere length regulation by KEOPS is independent of its t6A biosynthesis activity. Cytoplasmic overexpression of Qri7, a functional counterpart of KEOPS in mitochondria, restores cytosolic tRNA t6A modification and cell growth, but is not sufficient to rescue telomere length in the KEOPS mutant kae1Δ cells, indicating that a t6A modification-independent function is responsible for the telomere regulation. The results of our in?vitro biochemical and in?vivo genetic assays suggest that telomerase RNA TLC1 might not be modified by Sua5 and KEOPS. Moreover, deletion of KEOPS subunits results in a dramatic reduction of telomeric G-overhang, suggesting that KEOPS regulates telomere length by promoting G-overhang generation. These findings support a model in which KEOPS regulates telomere replication independently of its function on tRNA modification.Keywords: Telomere,
KEOPS complex,
Sua5,
G-overhang
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