Science China Life Sciences
Abstract
Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site ofTaGRF4, the resulting complex (mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of mTaGRF4-TaGIF1 together with a cytosine base editor targetingTaALSresulted in 2–9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, mTaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation.
| 论文编号: | DOI:10.1007/s11427-021-1949-9 |
| 论文题目: | Transient Expression of A TaGRF4-TaGIF1 Complex Stimulates Wheat Regeneration and Improves Genome Editing |
| 英文论文题目: | Transient Expression of A TaGRF4-TaGIF1 Complex Stimulates Wheat Regeneration and Improves Genome Editing |
| 第一作者: | Fengti Qiu, Sinian Xing, Chenxiao Xue, Jinxing Liu, Kunling Chen, Tuanyao Chai & Caixia Gao |
| 英文第一作者: | Fengti Qiu, Sinian Xing, Chenxiao Xue, Jinxing Liu, Kunling Chen, Tuanyao Chai & Caixia Gao |
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| 发表年度: | 2021-08-31 |
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| 摘要: | Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site ofTaGRF4, the resulting complex (mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of mTaGRF4-TaGIF1 together with a cytosine base editor targetingTaALSresulted in 2–9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, mTaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation. |
| 英文摘要: | Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site ofTaGRF4, the resulting complex (mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of mTaGRF4-TaGIF1 together with a cytosine base editor targetingTaALSresulted in 2–9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, mTaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation. |
| 刊物名称: | Science China Life Sciences |
| 英文刊物名称: | Science China Life Sciences |
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| 其它备注: | Fengti Qiu, Sinian Xing, Chenxiao Xue, Jinxing Liu, Kunling Chen, Tuanyao Chai & Caixia Gao. Transient Expression of A TaGRF4-TaGIF1 Complex Stimulates Wheat Regeneration and Improves Genome Editing. Science China Life Sciences. DOI:10.1007/s11427-021-1949-9 |
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