Bo He
Chengqi Yi
Jinying Peng
aState Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
bPeking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
cSynthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China
More InformationCorresponding author: E-mail address: chengqi.yi@pku.edu.cn (Chengqi Yi);E-mail address: jypengpku@pku.edu.cn (Jinying Peng)
Received Date: 2017-10-31
Accepted Date:2018-02-01
Rev Recd Date:2018-01-10
Available Online: 2018-03-08 Publish Date:2018-04-20
Abstract
Abstract
Analysis of patient's materials like cells or nucleic acids obtained in a minimally invasive or noninvasive manner through the sampling of blood or other body fluids serves as liquid biopsies, which has huge potential for numerous diagnostic applications. Circulating cell-free DNA (cfDNA) is explored as a prognostic or predictive marker of liquid biopsies with the improvements in genomic and molecular methods. DNA methylation is an important epigenetic marker known to affect gene expression. cfDNA methylation detection is a very promising approach as abnormal distribution of DNA methylation is one of the hallmarks of many cancers and methylation changes occur early during carcinogenesis. This review summarizes the various investigational applications of cfDNA methylation and its oxidized derivatives as biomarkers for cancer diagnosis, prenatal diagnosis and organ transplantation monitoring. The review also provides a brief overview of the technologies for cfDNA methylation analysis based on next generation sequencing.Keywords: Circulating cell-free DNA,
DNA methylation,
Cancer detection,
Biomarkers,
Methylation analysis methods
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