中文关键词:表面印迹粉煤灰微球氧氟沙星固相萃取 英文关键词:Surfacemolecular impringing, Ashmicrospheres, Ofloxacin, Solid-phaseextraction 基金项目:黑龙江省教育厅项目（12531757）

作者	单位	E-mail
苏立强	齐齐哈尔大学化学与化学工程学院 齐齐哈尔 161006	slq202@163.com
李晶	齐齐哈尔大学化学与化学工程学院 齐齐哈尔 161006
刘静禹	齐齐哈尔大学化学与化学工程学院 齐齐哈尔 161006
邹雪莹	齐齐哈尔大学化学与化学工程学院 齐齐哈尔 161006

摘要点击次数:1746 全文下载次数:0 中文摘要: 以工业废料粉煤灰微球为基质，氧氟沙星 (OFL) 为模板分子，采用表面印迹法制备印迹材料MIP。通过紫外光谱法结合理论分析选择实验条件，并对该印迹材料结构、吸附行为进行研究。结果表明，该印迹材料对氧氟沙星具有良好的特异识别性和优良的亲和性。与以硅胶为载体制备的印迹聚合物相比，该材料吸附容量更高和印迹效果更好。将其作为固相萃取填料对鸡产品进行分离富集，与C18柱相比，分离富集效果更好。结合UPLC，对实际样品中氧氟沙星进行分析，回收率为82.0%-96.7%，相对标准偏差低于5.5%，可用于鸡产品中氧氟沙星分离分析。 英文摘要: The molecularly imprinted polymer (MIP) had been synthesized using ofloxacin as the template molecule and the modified ash microspheres as carrier by surface molecular imprinting technology. Binding properties and structure of MIP were investigated by spectroscopy and static method respectively. The results demonstrated that the synthesized MIP possessed specific recognition selectivity and excellent binding affinity for ofloxacin. Compared with the surface imprinted material with silica gel as carrier, the prepared molecularly imprinted material had the characteristics of high adsorption capacity and favorable imprinted effect. The separation and enrichment of MIP as solid phase extraction (SPE) materials were better than that of C18. The SPE coupled with UPLC was used for analysis of ofloxacin in actual samples. The recoveries of the developed method were in the rang from 82.0 to 96.7%, and the RSD was less than 5.5%. The results showed that the method could be used for the separation and enrichment of ofloxacin in chicken products. 查看全文查看/发表评论下载PDF阅读器 相关附件：版权转让声明书 -->