光合膜复合物功能调控

于龙江 研究员

1980年9月出生于山东省文登市，2004年和2007年在天津理工大学分别获学士和硕士学位，2011年3月在日本茨城大学获博士学位。2011年4月至2014年1月在日本茨城大学进行博士后研究，2014年2月至2018年12月在日本冈山大学任助理教授，2018年12月到中国科学院植物研究所工作，已发表SCI论文30余篇。电话：
邮箱：longer@ibcas.ac.cn




研究方向
研究组成员
发表论文
著作专利

研究方向：光合细菌是地球上最早出现的生物之一，迄今已有30多亿年的历史，是研究光合作用的重要模式生物。地球上仍存在着大量在极端环境条件（酸、碱、热、寒、盐）下生长的光合细菌，这些微生物作为研究光合作用生理化学极限的理想模型，对于了解光合作用的起源与进化具有极其重要的意义。本实验室致力于探索极端光合微生物的光合作用及其相关的生理代谢网络相互关系等研究，运用生物化学、物理化学、结构生物学和分子生物学等学科的理论与技术，通过对关键蛋白复合物的结构与功能进行研究，揭示其中的分子机制，为进一步提高人工光电太阳能电池的捕光效率以及开发新型的生物燃料电池奠定理论基础。研究方向包括：（1）光合膜蛋白复合物的结构与功能（2）光合作用原初反应机制（3）光合生物极端环境适应分子机制

马菲
副研究员
fma@ibcas.ac.cn



邹美娟
工程师
mjzou@ibcas.ac.cn



齐晨辉
2019级 博士
qichenhui@126.com



王香平
2019级 硕士
@qq.com



慕鸿臣
2019级 硕士
thrudheim@163.com





Chen, J.H.*; Yu, L. J.*; Boussac, A.; Wang-Otomo, Z.Y.; Kuang T.; Shen, J. R.(2018). Properties and structure of a low-potential, penta-heme cytochrome c552 from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium tepidum. Photosynth. Res, (DOI 10.1007/s11120-018-0507-y, *equal contribution)

Kimura, Y.; Kawakami, T.; Arikawa, T.; Li, Y., Yu, L. J.; Ohno, T.; Madigan, M. T.; Wang-Otomo, Z. Y.(2018). C-terminal cleavage of the LH1 alpha-polypeptide in the Sr2+-cultured Thermochromatium tepidum. Photosynth. Res., 135, 23-31.

Yu, L. J.; Suga, M.; Wang-Otomo, Z.Y.; Shen, J. R.(2018). Novel features of LH1-RC from Thermochromatium tepidum revealed from its atomic resolution structure. FEBS Lett, 285, 4359-4366.

Yu, L. J.; Suga, M.; Wang-Otomo, Z.Y.; Shen, J. R. (2018). Structure of photosynthetic LH1-RC supercomplex at 1.9 ? resolution. Nature, 556 (7700), 209-213.

Yu, L. J.; Kawakami, T.; Kimura, Y.; Wang-Otomo, Z. Y.(2016). Structural basis for the unusual qy red-shift and enhanced thermostability of the lh1 complex from Thermochromatium tepidum. Biochemistry, 55 (47), 6495-6504.

Niwa, S.*; Yu, L. J.*; Takeda, K.; Hirano, Y.; Kawakami, T.; Wang-Otomo, Z. Y.; Miki, K.(2014). Structure of the LH1-RC complex from Thermochromatium tepidum at 3.0 ?. Nature, 508 (7495), 228-32. (*equal contribution)

Yu, L. J.; Unno, M.; Kimura, Y.; Yanagimoto, K.; Oh-oka, H.; Wang-Otomo, Z. Y.(2013). Structure analysis and characterization of the cytochrome c-554 from thermophilic green sulfur photosynthetic bacterium Chlorobaculum tepidum. Photosynth. Res., 118 (3), 249-258.

Yu, L. J.; Kato, S.; Wang, Z. Y.(2010). Examination of the putative Ca2+-binding site in the light-harvesting complex 1 of thermophilic purple sulfur bacterium Thermochromatium tepidum. Photosynth. Res., 106 (3), 215-220.

Yu, J.; Fu, L. M.; Yu, L. J.; Shi, Y.; Wang, P.; Wang-Otomo, Z. Y.; Zhang, J. P.(2017). Carotenoid singlet fission reactions in bacterial light harvesting complexes as revealed by triplet excitation profiles. J. Am. Chem. Soc., 139 (44), 15984-15993.

Suga, M.; Akita, F.; Sugahara, M.; Kubo, M.; Nakajima, Y.; Nakane, T.; Yamashita, K.; Umena, Y.; Nakabayashi, M.; Yamane, T.; Nakano, T.; Suzuki, M.; Masuda, T.; Inoue, S.; Kimura, T.; Nomura, T.; Yonekura, S.; Yu, L. J.; Sakamoto, T.; Motomura, T.; Chen, J. H.; Kato, Y.; Noguchi, T.; Tono, K.; Joti, Y.; Kameshima, T.; Hatsui, T.; Nango, E.; Tanaka, R.; Naitow, H.; Matsuura, Y.; Yamashita, A.; Yamamoto, M.; Nureki, O.; Yabashi, M.; Ishikawa, T.; Iwata, S.; Shen, J. R.(2017). Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL. Nature, 543 (7643), 131-135.

Nagashima, K. V. P.; Sasaki, M.; Hashimoto, K.; Takaichi, S.; Nagashima, S.; Yu, L. J.; Abe, Y.; Gotou, K.; Kawakami, T.; Takenouchi, M.; Shibuya, Y.; Yamaguchi, A.; Ohno, T.; Shen, J.-R.; Inoue, K.; Madigan, M. T.; Kimura, Y.; Wang-Otomo, Z.-Y.(2017). Probing structure–function relationships in early events in photosynthesis using a chimeric photocomplex. Proc. Natl. Acad. Sci., 114 (41), 10906-10911.

Ma, F.; Yu, L. J.; Llansola-Portoles, M. J.; Robert, B.; Wang-Otomo, Z. Y.; van Grondelle, R.(2017). Metal cations induced alphabeta-bchl a heterogeneity in lh1 as revealed by temperature-dependent fluorescence splitting. Chemphyschem, 18 (16), 2295-2301.

Ma, F.; Yu, L. J.; Hendrikx, R.; Wang-Otomo, Z. Y.; van Grondelle, R.(2017). Excitonic and vibrational coherence in the excitation relaxation process of two lh1 complexes as revealed by two-dimensional electronic spectroscopy. J. Phys. Chem. Lett., 8 (12), 2751-2756.

Ma, F.; Yu, L. J.; Hendrikx, R.; Wang-Otomo, Z. Y.; van Grondelle, R.(2017). Direct observation of energy detrapping in lh1-rc complex by two-dimensional electronic spectroscopy. J. Am. Chem. Soc., 139 (2), 591-594.

Ma, F.; Yu, L. J.; Ma, X. H.; Wang, P.; Wang-Otomo, Z. Y.; Zhang, J. P.(2016). Bacterial light-harvesting complexes showing giant second-order nonlinear optical response as revealed by hyper-rayleigh light scattering. J. Phys. Chem. B., 120 (35), 9395-401.

Kimura, Y.; Yura, Y.; Hayashi, Y.; Li, Y.; Onoda, M.; Yu, L. J.; Wang-Otomo, Z. Y.; Ohno, T.(2016). Spectroscopic and thermodynamic characterization of the metal-binding sites in the lh1-rc complex from thermophilic photosynthetic bacterium Thermochromatium tepidum. J. Phys. Chem. B., 120 (49), 12466-12473.

Huo, Y. L.; Shi, Y.; Wang, Q. Y.; Li, L.; Yu, L. J.; Wang, P.; Zhang, J. P.; Wang, Z. Y.(2016). Spectroscopic properties of LH2 from Thermochromatium tepidum in liposome and detergent micelles. Chem. J. Chin. Univ., 37 (9), 1678-1685.

Ma, F.; Yu, L. J.; Wang-Otomo, Z. Y.; van Grondelle, R.(2016). Temperature dependent LH1-->RC energy transfer in purple bacteria Tch. tepidum with shiftable LH1-Q band: A natural system to investigate thermally activated energy transfer in photosynthesis. Biochim. Biophys. Acta., 1857 (4), 408-414.

Shi, Y.; Zhao, N. J.; Wang, P.; Fu, L. M.; Yu, L. J.; Zhang, J. P.; Wang-Otomo, Z. Y.(2015). Thermal adaptability of the light-harvesting complex 2 from Thermochromatium tepidum: Temperature-dependent excitation transfer dynamics. J. Phys. Chem. B., 119 (47), 14871-14879.

Ma, F.; Yu, L. J.; Wang-Otomo, Z. Y.; van Grondelle, R.(2015). The origin of the unusual Qy red shift in LH1-RC complexes from purple bacteria Thermochromatium tepidum as revealed by Stark absorption spectroscopy. Biochim. Biophys. Acta., 1847 (12), 1479-1486.

Kimura, Y.; Kawakami, T.; Yu, L. J.; Yoshimura, M.; Kobayashi, M.; Wang-Otomo, Z. Y.(2015). Characterization of the quinones in purple sulfur bacterium Thermochromatium tepidum. FEBS Lett., 589 (15), 1761-5.

Kimura, Y.; Inada, Y.; Yu, L. J.; Ohno, T.; Wang, Z.(2013). Strontium ions are functionally replaceable with calcium ions in the light-harvesting 1 reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum. In Photosynthesis Research for Food, Fuel and the Future. (eds. Kuang, T. Y. et al.). 105-109 (Springer).

Sekine, F.; Horiguchi, K.; Kashino, Y.; Shimizu, Y.; Yu, L. J.; Kobayashi, M.; Wang, Z. Y.(2012). Gene sequencing and characterization of the light-harvesting complex 2 from thermophilic purple sulfur bacterium Thermochromatium tepidum. Photosynth. Res., 111 (1-2), 9-18.

Yang, F.; Yu, L. J.; Wang, P.; Ai, X. C.; Wang, Z. Y.; Zhang, J. P.(2011). Effects of Aggregation on the excitation dynamics of LH2 from Thermochromatium tepidum in aqueous phase and in chromatophores. J. Phys. Chem. B, 115 (24), 7906-7913.

Kimura, Y.; Inada, Y.; Yu, L. J.; Wang, Z. Y.; Ohno, T.(2011). A Spectroscopic variant of the light-harvesting 1 core complex from the thermophilic purple sulfur bacterium Thermochromatium tepidum. Biochemistry, 50 (18), 3638-3648.

Yang, F.; Yu, L. J.; Wang, P.; Ai, X. C.; Wang, Z. Y.; Zhang, J. P.(2010). Excitation Dynamics of the Light-Harvesting complex 2 from Thermochromatium tepidum. Acta. Phys-Chim. Sin., 26 (7), 2021-2030.

Ma, F.; Kimura, Y.; Yu, L. J.; Wang, P.; Ai, X. C.; Wang, Z. Y.; Zhang, J. P.(2009). Specific Ca2+-binding motif in the LH1 complex from photosynthetic bacterium Thermochromatium tepidum as revealed by optical spectroscopy and structural modeling. FEBS J.,276 (6), 1739-1749.

Kimura, Y.; Yu, L. J.; Hirano, Y.; Suzuki, H.; Wang, Z. Y.(2009). Calcium Ions are required for the enhanced thermal stability of the light-harvesting-reaction center core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum. J. Biol. Chem., 284 (1), 93-99.

Kimura, Y.; Hirano, Y.; Yu, L. J.; Suzuki, H.; Kobayashi, M.; Wang, Z. Y.(2008). Calcium ions are involved in the unusual red shift of the light-harvesting 1 Qy transition of the core complex in thermophilic purple sulfur bacterium Thermochromatium tepidum. J. Biol. Chem., 283 (20), 13867-13873.