植物抗盐分子机理与生物技术

李银心 研究员
1985年，北京大学生物系获学士学位；
1990年，北京林业大学园林系获硕士学位；
2001年，中国科学院植物研究所获博士学位；
1990年至今，历任中科院植物所研究实习员、助理研究员、副研究员、研究员（博导）；
1999-2000年，加拿大多伦多大学植物系高级访问****；
2007-2010年，天津大学兼职教授；
2001年被评为“863”计划突出贡献先进个人；
2004年评为中国科学院京区巾帼建功标兵；
近年来在国内外学术刊物上发表论文56篇，申请专利42项，其中已授权专利29项。

联系方式： E-mail：yxli@ibcas.ac.cn
研究方向
课题组成员

从事以抗盐为主线的植物抗逆机理及抗逆植物应用研究，包括：1.盐生植物耐高盐的分子机理以特定盐生植物盐角草（Salicornia）为材料，利用比较蛋白质组、转录组和基因组学方法，对盐应答相关蛋白和抗盐基因进行了系统的分离、鉴定和功能分析，揭示了盐角草蛋白共同参与植物的抗逆反应的动态调控网络以及盐渍生境下碳氮协同代谢的机理；克隆了多个耐盐基因，发现了耐盐相关基因提高植物抗病性的新功能，比较鉴别盐生植物和甜土植物来源的抗盐基因在表达调控和功能上的异同及相关机理；2.植物抗逆性的细胞及基因工程采用植物组织和细胞培养、突变体筛选，结合转基因技术，培育抗盐芹菜（Apium）、菾菜(Beta)、菜用蒲公英（Taraxacum）、番茄（Lycopersicum）等耐盐蔬菜新种质；3.耐盐植物的筛选驯化通过种质资源收集、耐盐品比、遗传稳定性分析，从现有栽培蔬菜和野生盐生植物类群中筛选具有作物潜力的种类，获得“海芦笋”（Salicornia）等耐海水蔬菜新种质；4.抗盐耐海水蔬菜新品种选育和滩涂海水栽培集成应用将抗盐蔬菜种质栽培与海水平衡营养及设施技术相结合，实现了用海水灌溉和海水无土栽培，并大规模种植的应用目标。

李银心 研究员
yxli@ibcas.ac.cn



冯娟娟 副研究员
jjfeng@ibcas.ac.cn



吕素莲 助理研究员
sulianlv@ibcas.ac.cn



江萍 工程师
jiangping@ibcas.ac.cn



多丽雅 博士生
dlya1990@163.com



娄腾雪 博士生
txlou@ibcas.ac.cn



张璇 博士生
zhangxuan@ibcas.ac.cn



郭子兢 博士生



林康祺 硕士生
lkq1996@126.com



衣泽 硕士生





研究论文
(#Co-first author, *Corresponding author)
Jia WT, Miao FF, Lv SL, Feng JJ, Zhou SF, Zhang X, Wang D, Li SZ, Li YX*. 2017. Identification for the capability of Cd-tolerance, accumulation and translocation of 96 sorghum genotypes. Ecotoxicology and Environmental Safety, 145: 391–397 [下载]

Feng JJ^#, Jia WT^#, Lv SL, Bao H, Li JH, Li DS, Zhu C, Li SZ, Li YX*. 2017. Comparative transcriptome combined with morphophysiological analyses revealed key factors for differential cadmium accumulation in two contrasting sweet sorghum genotypes. Plant Biotechnology Journal, 1-14 [下载]

Jia WT, Lv SL, Feng JJ, Li JH, Li YX*, Li SZ*. 2016. Morpho-physiological characteristics analysis demonstrated the potential of sweet sorghum (Sorghum bicolor (L.) Moench) in the phytoremediation of cadmium contaminated soils. Environmental Science and Pollution Research, 23: 18823-18831 [下载]

Lv SL, Jiang P, Wang D, Li YX*. 2016. H⁺-pyrophosphatase from Salicornia europaea enhances tolerance to low phosphate under salinity in Arabidopsis. Plant Signaling & Behavior, 11: e** [下载]

Guo J, Chen XY, Bao H, Li YX*. 2016. Photosynthetic and physiological responses of mangroves under an environmental deterioration. Acta Physiologiae Plantarum, 38: 140 [下载]

Nie LL^#, Feng JJ^#, Fan PX^#, Chen XY, Guo J, Lv SL, Bao H, Jia WT, Tai F, Jiang P, Wang JH, Li YX*. 2015. Comparative proteomics of root plasma membrane proteins reveals the involvement of calcium signalling in NaCl-facilitated nitrate uptake in Salicornia europaea. Journal of Experimental Botany, 66: 4497-4510 [下载]

Lv SL^#, Jiang P^#, Nie LL, Chen XY, Tai F, Wang D, Fan PX, Feng JJ, Bao H, Wang JH, Li YX*. 2015. H⁺-pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in Arabidopsis and wheat. Plant, Cell and Environment, 38: 2433-2449 [下载]

Bao H, Chen XY, Lv SL, Jiang P, Feng JJ, Fan PX, Nie LL, Li YX*. 2015. Virus-induced gene silencing reveals control of reactive oxygen species accumulation and salt tolerance in tomato by γ-aminobutyric acid metabolic pathway. Plant Cell and Environment, 38: 600-613 [下载]

Wang JH, Feng JJ, Jia WT, Chang S, Li SZ, Li YX*. 2015. Lignin engineering through laccase modification: a promising field for energy plant improvement. Biotechnology for Biofuels, 8: 145 [下载]

Feng JJ, Wang JH, Fan PX, Jia WT, Nie LL, Jiang P, Chen XY, Lv SL, Wan LC, Chang S, Li SZ, Li YX*. 2015. High-throughput deep sequencing reveals that microRNAs play important roles in salt tolerance of enhalophyte Salicornia europaea. BMC Plant Biology, 15: 63 [下载]

Chen XY, Bao H, Guo J, Jia WT, Li YX*. 2015. Overexpression of SeNHX1 improves both salt tolerance and disease resistance in tobacco. Plant Signaling & Behavior, 10: e993240 [下载]

Feng JJ*, Fan PX*, Jiang P, Lv SL, Chen XY, Li YX*. 2014. Chloroplast-targeted Hsp90 plays essential roles in plastid development and embryogenesis in Arabidopsis possibly linking with VIPP1. Physiologia Plantarum, 150: 292-307 [下载]

Chen XY, Bao H, Guo J, Jia WT, Tai F, Nie LL, Jiang P, Feng JJ, Lv SL, Li YX*. 2014. Na⁺/H⁺ exchanger 1 participates in tobacco disease defence against Phytophthora parasitica var. nicotianae by affecting vacuolar pH and priming the antioxidative system. Journal of Experimental Botany, 65: 6107-6122 [下载]

Fan PX, Nie LL, Jiang P, Feng JJ, Lv SL, Chen XY, Bao H, Guo J, Tai F, Wang JH, Jia WT, Li YX*. 2013. Transcriptome analysis of Salicornia europaea under saline conditions revealed the adaptive primary metabolic pathways as early events to facilitate salt adaptation. PLoS ONE, 8: e80595 [下载]

Fan PX, Nie LL, Jiang P, Feng JJ, Lv SL, Chen XY, Bao H, Guo J, Tai F, Wang JH, Jia WT, Li YX*. 2013. Transcriptome analysis of Salicornia europaea under saline conditions revealed the adaptive primary metabolic pathways as early events to facilitate salt adaptation. PLoS ONE, 8: e80595 [下载]

Jiang D, Huang LF, Zhang ZY, Zhang K, Lv SL, Li YX*. 2012. Inhibitory effects of halophyte Sesuvium portulacastrum on the marine diatom Skeletonema costatum. Allelopathy Journal, 29: 137-150 [下载]

Jiang D, Huang LF, Lin YQ, Nie LL, Lv SL, Kuang TY, Li YX*. 2012. Inhibitory effect of Salicornia europaea on the marine alga Skeletonema costatum. Science China Life Sciences, 55: 551-558 [下载]

Lv SL, Nie LL, Fan PX, Wang XC, Jiang D, Chen XY, Li YX*. 2012. Sodium plays a more important role than potassium and chloride in growth of Salicornia europaea. Acta Physiologiae Plantarum, 34: 503-513 [下载]

Lv SL, Jiang P, Chen XY, Fan PX, Wang XC, Li YX*. 2012. Multiple compartmentalization of sodium conferred salt tolerance in Salicornia europaea. Plant Physiology and Biochemistry, 51: 47-52 [下载]

Chen XY , Han HP, Jiang P, Nie LL, Bao H, FanPX, Lv SL, Feng JJ,Li YX*. 2011. Transformation of beta - lycopene cyclase genes from Salicornia europaea and Arabidopsis conferred salt tolerance in Arabidopsis and tobacco. Plant and Cell Physiology, 52: 909-921 [下载]

Chen XY, Nie LL, Bao H, Jiang P, Lv SL, Li YX*. 2011. Modified non-invasive micro-test electrophysiological technology for vacuolar H+ flux detection. Analytical Biochemistry, 418: 295-297 [下载]

Fan PX, Feng JJ, Jiang P, Chen XY, Bao H, Nie LL, Jiang D, Lv SL, Kuang TY, Li YX*. 2011. Coordination of carbon fixation and nitrogen metabolism in Salicornia europaea under salinity：comparative proteomic analysis on chloroplast proteins. Proteomics, 11: 4346-4367 [下载]

Fan PX, Wang XC, Kuang TY, Li YX*. 2009. An efficient method for the extraction of chloroplast proteins compatible for 2-DE and MS analysis. Electrophoresis, 30: 3024-3033 [下载]

Chang CS, Wang BL, Shi L, Li YX, Duo L, Zhang WH*. 2010. Alleviation of salt stress-induced inhibition of seed germination in cucumber (Cucumis sativus L.) by ethylene and glutamate. Journal of Plant Physiology, 167: 1152-1156 [下载]

Wang BL, Shi L, Li YX, Zhang WH*. 2010. Boron toxicity is alleviated by hydrogen sulfide in cucumber (Cucumis sativus L.) seedlings. Planta, 231: 1301-1309 [下载]

Bao H, Li YX*. 2010. Effect of stage-specific saline irrigation on greenhouse tomato production. Irrigation Science, 28: 421-430 [下载]

Song HM, Fan PX, Shi WL, Zhao RM, Li YX*. 2010. Expression of five AtHsp90 genes in Saccharomyces cerevisiae reveals functional differences of AtHsp90s under abiotic stresses. Journal of Plant Physiology, 167: 1172-1178 [下载]

Jiang D, Huang LF, Lin SQ, Zhang YK, Li YX*. 2010. Growth inhibition of marine alga Skeletonema costatum by euhalophyte Salicornia bigelovii. Allelopathy Journal, 25: 163-172 [下载]

Song HM, Zhao RM, Fan PX, Wang XC, Chen XY, Li YX*. 2009. Overexpression of AtHsp90.2, AtHsp90.5 and AtHsp90.7 in Arabidopsis thaliana enhances plant sensitivity to salt and drought stresses. Planta, 229: 955-964 [下载]

Wang XC, Fan PX, Song HM, Chen XY, Li XF, Li YX*. 2009. Comparative proteomic analysis of differentially expressed proteins in shoots of Salicornia europaea under different dalinity. Journal of Proteome Research, 8: 3331-3345 [下载]

Song HM, Fan PX, Li YX*. 2009. Overexpression of organellar and cytosolic AtHSP90 in Arabidopsis thaliana impaires plant tolerance to oxidative stress. Plant Molecular Biology Reporter, 27: 342-349 [下载]

Wang XC, Tian WM, Li YX*. 2008. Development of an Efficient Protocol of RNA Isolation from Recalcitrant Tree Tissues. Molecular Biotechnology, 38: 57-64 [下载]

Zhou SF, Chen XY, Zhang XG, Li YX*. 2008. Improved salt tolerance in tobacco plants by co-transformation of a betaine synthesis gene BADH and a vacuolar Na+/H+ antiporter gene SeNHX1. Biotechnology Letters, 369-376 [下载]

Han HP, Li YX, Zhou SF*. 2008. Overexpression of phytoene synthase gene from Salicornia europaea alters response to reactive oxygen species under salt stress in transgenic Arabidopsis. Biotechnology Letters, 1501-1507 [下载]

Zhou SF, Xue XN, Zhang XG, Li YX*. 2007. Physiological and growth responses of tomato progenies harboring betaine aldehyde dehydrogenase (BADH) gene to salt stress. Journal of Integrative Plant Biology, 49: 628-637 [下载]

Wang XC, Li XF, Li YX*. 2007. A modified Coommassie Brilliant Blue staining method at nanogram sensitivity compatible with proteomic analysis. Biotechnology Letters, 29: 1599-1603 [下载]

Wang XC, Li XF, Deng X, Han HP, Shi WL, Li YX*. 2007. A protein extraction method compatible with proteomic analysis for euhalophyte Salicornia europaea L. Electrophoresis, 28: 3976-3987 [下载]

Shi XL, Han HP, Shi WL, Li YX*. 2006. NaCl and TDZ are two key factors for the improvement of in vitro regeneration rate of Salicornia europaea L. Journal of Integrative Plant Biology, 48: 1185-1189 [下载]

Chang FQ, Liu XM, Li YX, Jia GX, Ma JJ, Liu GS, Zhu ZQ*. 2003. Changes in DNA base sequences in the mutant of Arabidopsis thaliana induced by low-energy N+ implantation. Science in china, 46: 503-512 [下载]

Chang FQ, Li YX, Liu XM, Liu GS*, Zhu ZQ. 2003. Characterization of variation induced by low-energy N+ and cloning of differential expressed cDNA of a mutant in Arabidopsis thaliana. Progress in Natural Science, 13: 271-275 [下载]

Jia GX, Zhu ZQ, Chang FQ, Li YX*. 2002. Transformation of tomato with the BADH gene from Atriplex improves salt tolerance. Plant Cell Reports, 21: 141-146 [下载]

贾伟涛, 吕素莲, 冯娟娟, 李银心, 李纪红, 李十中*. 2015. 利用能源植物治理土壤重金属污染. 中国生物工程杂志, 35: 88-95 [下载]

聂玲玲, 冯娟娟, 吕素莲, 江萍, 范鹏祥, 台方, 李银心*. 2012 .真盐生植物盐角草(Salicornia europaeaL.)对不同氮形态的响应.生态学报, 32: 5703-571 [下载]

张新果, 李银心, 陈华, 石武良. 2008. 药蒲公英（Taraxacum officinale Weber）耐1.5％ NaCl变异体的筛选及特性分析. 生物工程学报, 24: 262－271 [下载]

张新果, 陈显扬, 姜丹, 李银心. 2008. 耐盐药蒲公英(Taraxacum officinale Weber)愈伤组织筛选及生理生化特性分析. 生物工程学报, 25: 1202-1209 [下载]

姜丹, 李银心, 黄凌风, 曾凡荣, 张语克. 2008. 盐度和温度对厦门海区引种栽培北美海蓬子的影响. 植物学通报, 25: 533-542 [下载]

王旭初, 范鹏祥, 李银心. 2007. 一种质谱兼容性的简化胶内酶解方法. 植物生理与分子生物学学报, 33: 449-455 [下载]

宋红苗, 陈显扬, 李银心. 2007. 植物热激蛋白90的结构和功能.植物生理学通讯, 43: 1002-1008 [下载]

李肖芳, 韩和平, 王旭初, 范鹏祥, 李银心. 2006. 适用于盐生植物的双向电泳样品制备方法. 生态学报, 26: 1848-1853 [下载]

陈华, 李平, 刘晶, 李银心. 2005. 药蒲公英（Taraxacum officinale Weber）再生体系的建立和优化. 生物工程学报, 21: 244-249 [下载]

刘晶, 周树峰, 陈华, 韩和平, 李银心. 2005. 农杆菌介导的双价抗盐基因转化番茄的研究. 中国农业科学, 38: 1636-1644 [下载]

李平, 陈华, 李银心. 2005. 大车前（Plantago major）体外再生体系的建立和优化. 生物工程学报, 21: 916-922 [下载]

陈华, 李银心. 2004. 蒲公英研究进展和用生物技术培育耐盐蒲公英展望. 植物学通报, 21: 19-25 [下载]

吕慧颖, 李银心, 陈华, 刘晶, 李平, 杨庆凯. 2004. 番杏Na+/H+逆向转运蛋白基因的克隆及特性分析. 高技术通讯, 14: 26-31 [下载]

常凤启, 李银心, 刘选明, 刘公社*, 朱至清*. 2003. 低能N+注入诱导拟南芥变异及突变体特异表达cDNA克隆. 自然科学进展, 13: 259-262 [下载]

常凤启, 刘选明, 李银心, 贾庚祥, 马晶晶, 刘公社*, 朱至清*. 2003. The vaiations of DNA base sequences in the mutant of Arabidopsis thaliana induced by low-enegy N+ implantation. 中国科学C辑, 33: 117-124 [下载]

吕慧颖, 李银心, 孔凡江, 杨庆凯. 2003. 植物Na+/H+逆向转运蛋白研究进展. 植物学通报, 20: 363-369 [下载]

陈华, 李银心. 2003. 耐海水蔬菜新成员蒲公英. 植物学杂志, 9-10 [下载]

贾庚祥, 朱至清, 李银心. 2002. 甜菜碱与植物耐盐基因工程. 植物学通报, 19: 272-279 [下载]

朱至清, 李银心. 2001. 生物技术与耐海水蔬菜的追求. 植物学杂志, 6: 3-4 [下载]