植物基因表达调控

曲乐庆 研究员
1984年，沈阳农业大学获学士学位；
1987年，沈阳农业大学获硕士学位；
2000年，日本九州大学获农学博士学位；
2000年，中国科学院遗传与发育生物学研究所植物细胞与染色体工程国家重点实验室副研究员；
2001-2005年，日本国立农业生物资源研究所博士后研究员；
2005年至今，任中国科学院植物研究所植物基因表达调控机制创新研究组组长；
2011年至今，任植物分类与资源学报编委；
近年来在国际学术期刊上发表20余篇文章，获得授权国家发明专利14项。

联系方式： E-mail：lqqu@ibcas.ac.cn
研究方向
课题组成员

1、植物基因表达调控机制通过对控制基因表达起关键调控作用的启动子、转录因子等诸因子的研究，揭示植物种子中内源和外源基因表达的遗传调控机制。同时克隆植物种子特异性高强度表达启动子，探索基因高效稳定表达的机制，提高基因在植物种子中的表达和积累水平，为应用转基因方法获得高科技附加值作物新品种及分子农场研究奠定理论基础。此工作得到国家转基因生物新品种培育重大专项和国家自然科学基金的资助。2、水稻种子储藏蛋白胞内转运机制蛋白质分选与胞内运输是生物学的一个基本问题，其不仅在维持细胞的内稳态方面发挥着重要作用，还涉及到信号转导、器官发育等重要生命过程。利用遗传学、反向遗传学、生物化学、分子和细胞生物学等方法，解析水稻种子储藏蛋白胞内转运、修饰和储藏的遗传调控机制，具有重要理论意义。同时对实现种子中外源有用蛋白定向运输、定点储藏、提高外源蛋白表达和累积水平具有重要应用价值。此工作得到国家自然科学基金的资助。3、植物生物反应器与保健功能性作物创制通过外源基因高效稳定表达体系，在水稻种子中特异性表达生理活性成分，利用种子生物反应器生产重要重组蛋白，同时培育具有保健功效的水稻新品种，使人们在正常饮食中预防和减少疾病的发生，实现医食同源。对于增进人们健康、节省医疗费用等具有重大社会意义和经济效应。此工作得到国家转基因生物新品种培育重大专项和国家高技术研究发展计划（863）的资助。

曲乐庆 研究员
lqqu@ibcas.ac.cn



董祥柏 助理研究员
dongxb503@ibcas.ac.cn



田丽宏 助理研究员
tianlihong@ibcas.ac.cn



钱丹丹 助理研究员
Qiandandan2008K@163.com



熊硕 博士生
xiongshuo123@126.com



陈良珂 博士生
clkgogo@163.com



杨慧芳 博士生



崔帅 博士生



周楠 硕士生



李梅 硕士生



孔箫航 硕士生



许佳堃 硕士生





研究论文
(#Co-first author, *Corresponding author)
Zhang D, Yang HF, Wang XC, Qiu YJ, Tian LH, Qi XQ, Qu LQ*. 2020. Cytochrome P450 family member CYP96B5 hydroxylates alkanes to primary alcohols and is involved in rice leaf cuticular wax synthesis. New Phytologist, 225: 2094-2107 [下载]

Tian LH, Xing YP, Fukuda M, Li R, Kumamaru T, Qian DD, Dong XB, Qu LQ*. 2018. A conserved motif is essential for the correct assembly of proglutelins and for their export from the endoplasmic reticulum in rice endosperm. Journal of Experimental Botany, 69:5029-5043 [下载]

Qian DD, Chen GQ, Tian LH, Qu LQ*. 2018. OsDER1 is an ER-associated protein degradation factor that responds to ER stress. Plant Physiology, 178:402-412 [下载]

Wang XC, Guan YYY, Zhang D, Dong XBi, Tian LH, Qu LQ*. 2017. A β-ketoacyl-CoA synthase is involved in rice leaf cuticular wax synthesis and requires a CER2-LIKE protein as a cofactor. Plant Physiology, 173: 944-955 [下载]

Li S, Wang X, He S, Li J, Huang Q, Imaizumi T, Qu LQ, Qin G, Qu LJ, Gu HY*. 2016. CFLAP1 and CFLAP2 are two bHLH transcription factors participating in synergistic regulation of AtCFL1-mediated cuticle development in Arabidopsis. PLoS Genetics, 12: e** [下载]

Dong XB, Zhang D, Liu J, Liu QQ, Liu HL, Tian L, Jiang L, Qu LQ*. 2015. Plastidial disproportionating enzyme participates in starch synthesis in rice endosperm by transferring maltooligosyl groups from amylose and amylopectin to amylopectin. Plant Physiology, 169: 2496-2512 [下载]

Qian DD, Tian L, Qu LQ*. 2015. Proteomic analysis of endoplasmic reticulum stress responses in rice seeds. Scientific Reports, 5: 14255 [下载]

Xu XP, Liu H, Tian L, Dong XB, Shen SH, Qu LQ*. 2015. Integrated and comparative proteomics of high-oil and high-protein soybean seeds. Food Chemistry, 172: 105-116 [下载]

Yin ZJ, Liu HL, Dong XB, Tian L, Xiao L, Xu YN, Qu LQ*. 2014. Increasing α-linolenic acid content in rice bran by embryo-specific expression of ω-3/Δ15-desaturase gene. Molecular Breeding, 33: 987-996 [下载]

Tian L, Dai LL, Yin ZJ, Fukuda M, Kumamaru K, Dong XB, Xu XP, Qu LQ*. 2013. Small GTPase Sar1 is crucial for proglutelin and α-globulin export from endoplasmic reticulum in rice endosperm. Journal of Experimental Botany, 64: 2831-2845 [下载]

Liu WX, Liu HL, Qu LQ*. 2013. Embryo-specific expression of soybean oleosin altered oil body morphogenesis and increased lipid content in transgenic rice seeds. Theoretical and Applied Genetics, 126: 2289-2297 [下载]

Liu HL, Yin ZJ, Xiao L, Xu YN, Qu LQ*. 2012. Identification and evaluation of ω-3 fatty acid desaturase genes for hyper-fortifying α-linolenic acid in transgenic rice seed. Journal of Experimental Botany, 63: 3279-3287 [下载]

Zhou QY, Wang L, Cai X, Wang D, Hua XJ, Qu LQ, Lin JX,Chen T*. 2011. Net sodium fluxes change significantly at anatomically distinct root zones of rice (Oryza sativa L.) seedlings. Journal of Plant Physiology, 168: 1249-1255 [下载]

Li WJ, Dai LL, Chai ZJ, Yin ZJ, Qu LQ*. 2011. Evaluation of seed storage protein gene 3’-untranslated regions in enhancing gene expression in transgenic rice seed. Transgenic Research, 21: 545-553 [下载]

Li XJ, Yang MF, Chen H, Qu LQ, Chen F, Shen SH*. 2010. Abscisic acid pretreatment enhances salt tolerance of rice seedlings: Proteomic evidence. BBA Protein Proteom, 1804: 929-940 [下载]

Liu WX, Liu HL, Chai ZJ, Xu XP, Song YR, Qu LQ*. 2010. Evaluation of seed storage protein gene 5'-UTR in enhancing gene expression in transgenic rice seed. Theoretical and Applied Genetics, 121: 1267-1274 [下载]

Wei JH, Wang YZ, Wang HZ, Li RF, Lin N, Ma RC, Qu LQ, Song YR*. 2008. Pulping performance of transgenic poplar with depressed Caffeoyl-CoA O-methyltransferase. Chinese Science Bulletin, 53: 3553-3558 [下载]

Qu LQ, Xing YP, Liu WX, Xu XP, Song YR*. 2008. Expression pattern and activity of six glutelin gene promoters in transgenic rice. The Journal of Experimental Botany, 59: 2417-2424 [下载]

Qu LQ, Yoshihara T, Ooyama A, Goto Y, Takaiwa F*. 2005. Iron accumulation does not parallel the high expression level of ferritin in transgenic rice seeds. Planta, 222: 225-233 [下载]

Cheng Y, Li H, Li HJ, Li YW, Zhu YF, Qu LQ, Jia X*. 2004. Expression of rice glutelin gene GluA-2 in wheat endosperm. Acta Botanica Sinica, 46: 1220-1225 [下载]

Qu LQ, Takaiwa F*. 2004. Evaluation of tissue specificity and expression strength of rice seed component gene promoters in transgenic rice. Plant Biotechnology Journal, 2: 113-125
[下载]

Qu LQ, Tada Y, Takaiwa F*. 2003. In situ Western hybridization: a new highly sensitive technique to detect protein distribution in seeds. Plant Cell Reports, 22: 282-285 [下载]

Qu LQ, Wei XL, Satoh H, Kumamaru T, Ogawa M, Takaiwa F*. 2003. Biochemical and molecular characterization of a rice glutelin allele for the GluA-1 gene. Theoretical and Applied Genetics, 107: 20-25 [下载]

Qu LQ, Wei XL, Satoh H, Kumamaru T, Ogawa M, Takaiwa F*. 2002. Inheritance of alleles for glutelina -2 subunit genes in rice and identification of their corresponding cDNA clone. Theoretical and Applied Genetics, 105: 1099-1108 [下载]

Qu LQ, Wei XL, Satoh H, Kumamaru T, Ogawa M, Jia X*. 2001. New rice mutants lacking glutelin α-1 subunit. Acta Genetica Sinica, 28: 229-235 [下载]

Qu LQ, Wei XL, Satoh H, Kumamaru T, Ogawa M, Jia X*. 2001. Micro-heterogeneity of rice seed storage glutelin. Acta Botanica Sinica, 43: 815-820 [下载]