doi:10.12202/j.0476-0301.2020177朱丹^1,,,
赵宝林¹,
韩科学²,
赵晓²
1.安徽中医药高等专科学校； 241000，安徽芜湖
2.北京师范大学水科学研究院， 100875，北京
基金项目:安徽省高校自然科学研究重点项目（KJ2019A1085）

详细信息

通讯作者:朱丹（1982—），女，副教授. 研究方向：生物化学与环境化学. e–mail: zhudan20111@126.com

中图分类号:S718.43；Q945.78

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出版历程

收稿日期:2020-01-01
网络出版日期:2020-09-08
刊出日期:2020-08-25

Effects of water and high temperature stress on active oxygen metabolism and total flavonoids accumulation in Mentha haplocalyx seedlings

Dan ZHU^1,,,
baolin ZHAO¹,
Kexue HAN²,
Xiao ZHAO²
1. Anhui College of Traditional Chinese Medicine, 241000, Wuhu, Anhui, China
2. College of Water Sciences, Beijing Normal University, 100875, Beijing, China



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摘要
摘要:水资源紧缺和全球气候变暖趋势，使药用植物同时面临着水分及高温胁迫. 本文以薄荷幼苗为研究对象，通过光照培养箱模拟胁迫环境，用聚乙二醇（PEG6000）模拟水分单一胁迫，设置40℃为高温单一胁迫，二者交互为复合胁迫，蒸馏水、25℃为对照，主要测定0~24 h超氧阴离子（${\rm{O}}_2\cdot^- $）产生速率，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性，过氧化氢（H₂O₂）、丙二醛（MDA）质量摩尔浓度，以及黄酮的质量分数变化. 结果显示：水分及高温单一、复合胁迫处理下，${\rm{O}}_2\cdot^- $产生速率、H₂O₂质量摩尔浓度、SOD、POD和CAT活性表现为先升后降的趋势，单一胁迫MDA质量摩尔浓度为整体上升趋势，复合胁迫MDA先升高后降低；4~12 h之间，复合胁迫各检测值显著高于单一胁迫，表现为协同效应；24 h复合胁迫下所有检测值先于单一胁迫降低至（甚至低于）对照水平. 可能是由于复合胁迫伤害大于单一胁迫伤害，随着时间的延长，率先突破薄荷幼苗忍受临界点导致死亡. 黄酮累积的变化表现为从4 h上升至8 h质量分数最大，到12 h显著下降，与${\rm{O}}_2\cdot^- $产生速率、H₂O₂的质量摩尔浓度分别在4、8 h达到最大，到12 h显著降低的趋势一致，提示胁迫条件下黄酮的累积很有可能是因为活性氧的产生而上调的. 研究表明：水分、高温胁迫可以通过诱导抗氧化酶来保护薄荷幼苗因活性氧的产生而导致的伤害，但是有限的；活性氧同时又可能提升幼苗黄酮的累积. 因此，在实际生产应用中，不仅要考虑到胁迫的协同效应，还要考虑胁迫的时间效应，从而平衡植物“产量”与“质量”的最佳方案.
关键词:水分胁迫/
高温胁迫/
薄荷/
活性氧/
抗氧化酶/
丙二醛/
总黄酮
Abstract:Shortage of water resources and trend of global warming make medicinal plants face water and high temperature stress at the same time. Mentha haplocalyx seedlings were subject to stress environment in a light incubator, with polyethylene glycol (PEG6000) used as single stress of water, and 40 ℃ as single stress of high temperature, together with compound stress of the two. Pure water and 25 ℃ were used as controls. Production of superoxide anion (${\rm{O}}_2\cdot^- $), hydrogen peroxide (H₂O₂), contents of superoxide dismutase (SOD) and peroxidase (POD), catalase (CAT), as well as malondialdehyde (MDA) and flavone levels were measured. Under single and complex stress of water and high temperature, MDA, ${\rm{O}}_2\cdot^- $, H₂O₂, SOD, POD and CAT increased first and then declined, MDA under single stress increased overall, MDA under complex stress increased first and then decreased. Values under complex stress were significantly higher than under single stress between 4 and 12 h, showing synergistic effects. Under 24 h of combined stress, all detected values decreased down to or even lower than controls before single stress, likely due to greater damage by combined stress than single stress. With extended times, a critical point of tolerance was reached and mint seedling death was seen. Flavone accumulation showed increase from 4 to 8 h then decrease from 8 to 12 h, consistent with levels of ${\rm{O}}_2\cdot^- $ and H₂O₂ at 4 and 8 h, respectively. Accumulation of flavone under stress was probably up-regulated by active oxygen. Water and high temperature stress could therefore induce antioxidant enzymes to protect peppermint seedlings, to a limited extent. But reactive oxygen species may enhance accumulation of flavonoids. Therefore, not only synergistic effect of multiple stress, but also time effect of stress should be considered to balance “yield” and “quality” of plants.
Key words:water stress/
high temperature stress/
Mentha haplocalyx/
reactive oxygen/
antioxidant enzymes/
MDA/
total flavonoids