1998-2012 Focusing on the mechanism of ovine and bovine oocyte maturation in vitro by researching on the effects of EGF, PG and FSH AND LH on the development of their pre-implantation embryos. In vitro production of transgenic cloning of beef cattles.
Grant Support
National Fund for Natural Sciences of China ( 31072185, PI ) 2011/01/01 – 2013/12/31
The goal of this project is to explore the mechanism of the regulation of differential embryonic expression of IFNτ on different types of bovine embryos, and their in vitro induction of ISG15 on their uterine epithelium cells cultured in vitro. [ $5,600,0]
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant (2009ZX08007-005B, PI ) 2009/01/01—2012/7/31
The goal of this project is to breed a new transgenic clonig beef cattle breed. [ $8,340,0]
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant ( 2011ZX08008-005-03, PI ) 2011/08/01—2012/12/31
The goal of this project is to breed a new transgenic anti-arthritis encephalitis ovine breed. [ $8,340,0]
National Key Scientific and Technological Grant of China for Breeding New Transgenic Animals and Plants---Sub-grant ( 2008ZX08008-005-02, Co-investigator, PI: Liu Yun-hai )
The goal of this project is to breed a new transgenic anti-arthritis encephalitis ovine breed. [ $19,400,0]
2008/08/01
National Eleventh “5-year-plan” “863 High Tech Major Plan” of China---Sub-grant ( 2008AA1003， PI)
The goal of this project is to explore the mechanism of in vitro maturation for ovine oocytes . [ $4,500,0]
2009/01/01
PUBLICATIONS
1. Zhang Shaoyu, Liu Yunhai, Ni Hemin, Wang Hao, Gu Meichao, Yan Peng, Yang Fei,Guo Yong, Gene expression profile analysis of dormant mouse embryos preserved by controlled slow freezing, Acta Laboratorium Animals Scientia Sinica ,2012, 20(5): 15-19,
2. Chen Li-ping, Piao Xue-jiao, Luo Yong, Ni He-min, Liu Yun-hai, Guo Yong, Study on the in-vitro cultured methods for the uterine endometrical cells of dairy cattles and sheep, Chinese Agricultural Science Bulletin, 2012, 28(14): 99-104,
3. Li Kai#, Ni He-min#, Liu Yun-hai, Zhao Yan-wei, Qu Yang-yan, Li Xiao-yan, Guo Yong, LH plays a role to enhance the nuclear and cytoplasm synchronization for in vitro maturation of ovine oocytes, Scientia Agricultura Sinica,2011, 10（1）：136-141；(#：co-first author, *: correspondence author, indexed by SCIE)
4. Ni He-min*, Zhang Hong-bo, Liu Yun-hai, Deng Gui-xin, Lu Tian-gang, Guo Yong, The distribution of LH receptor on the ovaries among three different sheep breeds by different efficiency of superovulation, Acta Veterinaria et Zootechnica Sinica, 2011, 42(11): 1625-1631, (*: the first author and correspondence author)
5. Ni He-min*, Zhang Hong-bo, Liu Yun-hai, Lu Tian-gang, Deng Gui-xin, Guo Yong, Distribution of FSH receptor on the ovaries among different sheep breeds by different efficiency of superovulation, Scientia Agricultura Sinica,2011, 44（6）：1232-1238；
6. Tian Yu-jing, Lu Wen-geng, Zhang Yu, Lin Hao, Ni He-min, Guo Yong, Isolation and cultivation of bovine trophoblastic stem-like cell lines, Chinese Agricultural Science Bulletin, 2011, 27(26): 20-24,
7. Lin ZL, Gao Y, Deng GX, Liang HB, Zhang HB, Liu YH, Ni HM, Guo Y, Wang ZHG, Effect of estrus synchronization with PMSG and FSH on the distribution of estrogen receptor α in the mouse uterus, ovary and fallopian tube, Acta Laboratorium Animals Scientia Sinica ,2010, 18(3): 204-207,
8. Gao Y, Lin ZL, Zhang HB, Liang HB, Liu YH,Guo Y, Ni He-min*, Comparison of the expression of estrogen receptor α in mouse endometrium between those treated by estrus synchronization and in natural estrus, Acta Laboratorium Animals Scientia Sinica ,2010, 18(2): 168-171,
9. Liang HB, Liu JQ, Li FH, Xu HT, Niu JY, Li J, Sun Y, Guo Y, Liu YH, Comparison of different methods for boar sperm capacitation by CTC staining, China Animal Husbandry and Veterinary Medicine, 2010, 37(4): 171-173,
10. Liu YJ, Xia Y, Jin JN, Guo R, Liu YH, Guo Y, Li JH, Wei XL, Effect od different durations treated by luteinizing hormone on maturation in vitro of bovine oocytes, China Animal Husbandry and Veterinary Medicine, 2010, 37(5): 123-125,
11. Liu LQ, Guo Y, Liu YH, Xue JZH, Zhang H, Li XY, Ni HM, Effect of Calcium Ionophore and Caffeine on Ovine Sperm Capacitation in vitro, China Animal Husbandry and Veterinary Medicine, 2010, 37(1): 112-115,
12. Li K#, Ni HM#, Liu YH, Zhao YW, Qu YY, Li XY, Guo Y, LH plays a role to enhance the nuclear and cytoplasm synchronization for in vitro maturation of ovine oocytes, Scientia Agricultura Sinica,2009(10): 42(10):3633-3638, (*: correspondence author)
