二维码（扫一下试试看！） | | 全脑辐照致神经损伤大鼠神经化学物质类生物标志物的筛选 | | Screening of Neurochemical Biomarkers for Rats with Nerve Injury Induced by Whole-Brain Irradiation | | 投稿时间：2021-03-21 | | DOI：10.15918/j.tbit1001-0645.2021.074 | | 中文关键词:全脑辐照血清神经化学物质生物标志物 | | English Keywords:whole-brain irradiationserumneurochemicalbiomarker | | 基金项目:国家自然科学基金资助项目（U1532264，81741132） | | | 摘要点击次数:373 | | 全文下载次数:241 | | 中文摘要: | | 为了对全脑辐照诱导神经损伤后的大鼠血进行外周血中神经化学物质类生物标志物的筛选，采用前期建立的血清中神经化学物质的化学衍生-超高效液相色谱-串联质谱分析方法，对不同全脑辐照剂量（0、10、30 Gy）和分次累积辐照（10 Gy×3）大鼠血清中42种神经化学物质的浓度进行了测定并进行了基于靶向代谢组学策略的统计学分析.单变量统计分析结果表明，辐照组（10、30、10 Gy×3）中去甲肾上腺素、乙酰胆碱、γ-氨基丁酸、酪胺、谷氨酸、酪氨酸、色氨酸、褪黑素、α-丙氨酸和甘氨酸的含量显著（p<0.05）低于对照组（0 Gy），而乙酰胆碱和褪黑素的浓度变化趋势相反（p<0.05）.同时发现10 Gy×3组中神经化学物质含量的变化程度低于30 Gy组，表明分次累积辐照造成的神经化学物质扰动程度较同剂量的单次辐照低；通过结合多变量统计OPLS-DA模型分析中的变量重要性投影（VIP）值，本研究筛选出乙酰胆碱、谷氨酸、酪胺和褪黑素（p<0.05，VIP>1）可作为全脑辐照诱导神经损伤的潜在生物标志物. | | English Summary: | | Screening of neurochemical biomarkers was performed in peripheral blood serum from rats with nerve injury induced by whole-brain irradiation(WBI).Serum of rats treated with different doses of WBI(0,10,and 30 Gy) and fractional cumulative radiation(10 Gy×3) were determined for the concentration of 42 neurochemicals using the previously established chemical derivatization and ultra-high performance liquid chromatography-tandem mass spectrometry method followed by statistical analysis based on targeted metabolomics. Univariate statistical analysis indicated that the concentrations of norepinephrine, acetylcholine, γ-aminobutyric acid, tyramine, glutamic acid, tyrosine, tryptophan, melatonin, α-alanine, and glycine were significantly (p<0.05) lower than the control group (0 Gy), while the concentrations of acetylcholine and melatonin changed in the opposite trend (p<0.05); Meanwhile the variation degree of neurochemicals in the 10 Gy×3 group was found lower than that in the 30 Gy group, indicating that the disturbance degree of neurochemicals caused by cumulative irradiation was lower than that caused by single irradiation of the same dose. Combining with the variable importance in the projection(VIP) in the multivariate statistical OPLS-DA model, the proposed method has selected acetylcholine,glutamate,tyramine,and melatonin as the potential biomarkers for the nerve injury induced by WBI. | | 查看全文查看/发表评论下载PDF阅读器 | |
岳光,潘玉田,张华君,刘学杰,李宇峰.多神经元神经网络算法的DSP无人侦察车伺服控制系统[J].北京理工大学学报（自然科学版）,2019,39(2):203~208.YUEGuang,PANYu-tian,ZHANGHua-jun,LIUXue-jie,LIYu-feng.AMulti-Neuron ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21张惠瑾,杨艳玲,李星,刘永旺,赵锂.银离子-热力复合冲击消毒对生活热水管壁生物膜的控制作用[J].北京理工大学学报（自然科学版）,2019,39(6):655~660.ZHANGHui-jin,YANGYan-ling,LIXing,LIUYong-wang,ZHAOLi.TheControlEff ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21宋宇波,彭朝阳,苏悦,刘蕴箫,赵千锋,朱珍超.基于神经网络模型的室内大规模人流密度预测[J].北京理工大学学报（自然科学版）,2019,39(7):714~718,769.SONGYu-bo,PENGChao-yang,SUYue,LIUYun-xiao,ZHAOQian-feng,ZHUZhen- ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21吴鹏,孟祥远,王子晔,高建伟.基于神经动力学的冗余机械臂恒定转速比算法[J].北京理工大学学报（自然科学版）,2019,39(10):1081~1085.WUPeng,MENGXiang-yuan,WANGZi-ye,GAOJian-wei.ConstantRotationRatioofRedund ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21.北京理工大学学报2019年总目次（第39卷）[J].北京理工大学学报（自然科学版）,2019,39(12):1321~1338..[J].TransactionsofBeijingInstituteofTechnology,2019,39(12):1321-1338.二维码（扫一下试试看！）北京理 ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21张帅,高昕宇,尹宁,郭梁,徐桂芝.基于TMAES对GrC神经元放电活动影响的仿真[J].北京理工大学学报（自然科学版）,2019,39(S1):19~27.ZHANGShuai,GAOXin-yu,YIN-Ning,GUOLiang,XUGui-zhi.SimulationResearchonCer ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21周迪,李瑞,邓玉林.微流控芯片加工工艺及生物相容性探究[J].北京理工大学学报（自然科学版）,2019,39(S1):122~125.ZHOUDi,LIRui,DENGYu-lin.ProcessofMicrofluidicFabricationandBiocompatibilityExplorat ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21庞宇,侯虹宇,王慧倩,张诚,勾俊全,袁鹏飞.基于1/f特征的生物电刺激仪研制[J].北京理工大学学报（自然科学版）,2019,39(S1):136~140.PANGYu,HOUHong-yu,ZHANGCheng,GOUJun-quan,YUANPeng-fei.ResearchandDesigno ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21郭苗苗,齐志光,王磊,金铭,徐桂芝.语言刺激对镜像神经系统激活情况的研究[J].北京理工大学学报（自然科学版）,2019,39(S1):181~185.GUOMiao-miao,QIZhi-guang,WANGLei,JINGMing,XUGui-zhi.ResearchontheActivatio ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21诸云,王建宇,杨莹,刘博航.城市道路交通拥堵的模糊神经网络评析[J].北京理工大学学报（自然科学版）,2018,38(5):487~492.ZHUYun,WANGJian-yu,YANGYing,LIUBo-hang.FuzzyEvaluationofUrbanTrafficCongestionBa ... 北京理工大学科研学术 本站小编 Free考研考试 2021-12-21
| |
