Agricultural activities, including stock-farming, planting industry, and fish aquaculture, can affect the physicochemical and biological characters of freshwater lakes. However, the effects of pollution producing by agricultural activities on microbial ecosystem of lakes remain unclear. Hence, in this work, we selected Honghu Lake as a typical lake that is influenced by agriculture activities. We collected water and sediment samples from 18 sites, which span a wide range of areas from impacted and less-impacted areas. We performed a geospatial analysis on the composition of microbial communities associated with physicochemical properties and antibiotic pollution of samples. The co-occurrence networks of water and sediment were also built and analyzed. Our results showed that the microbial communities of impacted and less-impacted samples of water were largely driven by the concentrations of TN, TP, NO3?-N, and NO2?-N, while those of sediment were affected by the concentrations of Sed-OM and Sed-TN. Antibiotics have also played important roles in shaping these microbial communities: the concentrations of oxytetracycline and tetracycline clearly reflected the variance in taxonomic diversity and predicted functional diversity between impacted and less-impacted sites in water and sediment samples, respectively. Furthermore, for samples from both water and sediment, large differences of network topology structures between impacted and less-impacted were also observed. Our results provide compelling evidence that the microbial community can be used as a sentinel of eutrophication and antibiotics pollution risk associated with agricultural activity; and that proper monitoring of this environment is vital to maintain a sustainable environment in Honghu Lake.
农业生产活动，包括畜牧养殖、种植业和鱼类养殖等活动。农业生产活动中产生的污染会影响湖泊的理化性质和生态系统。先前的研究大多集中在农业生产活动对湖泊的宏观生态系统的影响上，而农业活动产生的污染对湖泊微生物生态系统影响研究却比较少，特别是农业生产活动中的抗生素对湖泊微生态的影响还缺少相关的研究。在本文中，我们选择洪湖这一典型的湖泊作为研究农业活动对湖泊微生态系统研究的对象，探究农业生产活动中的污染对湖泊微生态的影响。我们收集了洪湖及其附近的河流和池塘的18个位点的水样和底泥样本，共28个，水样和底泥样本各14个。这些样本依据农业生产活动对其影响的程度可分为受影响程度高（impacted）和受影响程度低（less-impacted）的水样和底泥样本。使用高通量测序技术对这些样本中微生物的16S rRNA V4-V5区进行测序，解析出这些样本的微生物群落组成和功能组成，并将理化因素和抗生素污染与微生物群落组成进行了关联分析，探索了农业生产活动对湖泊微生态影响及其在空间位置上的规律。结果表明，水体中的TN、TP、NO3--N和NO2--N是影响水体微生物群落结构的主要因素，而底泥中的Sed-OM和Sed-TN则是影响底泥微生物群落结构的主要驱动力。我们揭示了抗生素对于湖泊微生态的影响，其中土霉素和四环素是造成水体和底泥样本微生物群落结构的重要因素。我们筛选出可以用来表征农业生产活动对湖泊微生态影响程度的生物标志物。此外，我们也构建了微生物共出现网络，基于共出现网络拓扑结构的分析揭示了农业生产活动对微生物群落中微生物的影响。我们的结果表明微生物群落可以作为农业污染的响应器，而对环境的适当监测对于维持洪湖环境的可持续发展至关重要。





PDF全文下载地址:

http://gpb.big.ac.cn/articles/download/691