王宏达
单位：中国科学院长春应用化学研究所电分析化学国家重点实验室
地址：吉林省长春市人民大街5625号
邮编：130022
电话：86-
个人主页： http://yjsb.ciac.jl.cn/daoshi_read.php?brow=68&lb=%B5%BC%CA%A6%BC%F2%BD%E9
实验室介绍：


个人简历 Personal resume


王宏达，中国科学院长春应用化学研究所研究员，中国科学院“国家****基金”、“科技部中青年科技创新领军人才”获得者。1995年毕业于吉林大学分子生物学系获学士学位，2000年毕业于中科院长春应用化学研究所获分析化学博士学位，2001-2007年先后在德国马普分子生理研究所和美国亚利桑那州立大学从事博士后研究。长期从事单分子生物物理方面的研究工作，提出新的红细胞膜结构模型（Semi-mosaic model）和有核组织细胞膜结构模型（Protein Layer-Lipid-Protein Island model）；设计开发了基于原子力显微镜的测量动态微观力系统（“力示踪”技术），实时记录了细胞膜转运单分子/单颗粒的过程；自主研发超分辨荧光成像和原子力显微镜的联用技术。在Chemical Society Reviews、Nature Communications、Nano Letters、Cell Research、JACS、PNAS等杂志发表论文90余篇，申请和授权发明专利11项。近五年共发表论文55篇（25篇IF>5，4篇IF>10），其中通讯联系人和第一作者论文43篇。论文总被引1600余次，其中2篇论文单篇引用分别为290和190余次。应邀担任国际期刊Scientific Reports的编委、中国化学会生物物理化学专业委员会委员、中国生物物理学会单分子生物学分会理事、国家自然基金委面上/青年/地区项目会评专家、科技部重点研发专项会评专家。


研究方向 Research direction


1、分析化学
2、生物物理
3、细胞生物学



招生信息 Enrollment information


专业背景：分析化学，细胞生物学，生物化学，物理学等；考试科目：同中国科技大学招生考试科研条件：课题组拥有AFM5500、AFM7500原子力显微镜各一台，适合在液相条件下对生物样品进行操作，同时也具备完善的显微操作系统和超分辨荧光显微镜。此外，课题组隶属于电分析化学国家重点实验室，可以利用的资源包括：Multimode Ⅷ原子力显微镜、Icon原子力显微镜、共聚焦荧光倒置显微镜、细胞培养室、流式细胞仪、PCR、FTIR、圆二色光谱仪、紫外-可见光谱仪、荧光光谱仪和常规实验设备。中科院长春应用化学研究所拥有电子显微镜和质谱等实验仪器可供使用。学生待遇：同中国科学院长春应用化学研究所研究生待遇。


论文专著 The monograph


1)Developing substrate-based small molecule fluorescent probes for super-resolution fluorescent imaging of various membrane transporters - Nanoscale Horizons - 2020 - 2020年第三期
2)Structural Mechanism Analysis of Orderly and Efficient Vesicle Transport by High-Resolution Imaging and Fluorescence Tracking - Analytical Chemistry - 2020 - 2020年第九期
3)Quantitatively mapping the assembly pattern of EpCAM on cell membranes with peptide probes - Analytical Chemistry - 2020 - 2020年第二期
4)Mechanical force regulation of YAP by F-actin and GPCR revealed by super-resolution imaging - Nanoscale - 2020 - 2020年第四期
5)Correlative dual-color dSTORM/AFM reveals protein clusters at the cytoplasmic side of human bronchial epithelium membranes - Nanoscale - 2020 - 2020年第十八期
6)Development of small molecule inhibitor-based fluorescent probes for highly specific super-resolution imaging - Nanoscale - 2020 - 2020年第四十二期
7)Correlative dual-alternating-color photoswitching fluorescence imaging and AFM enable ultrastructural analyses of complex structures with nanoscale resolution - Nanoscale - 2020 - 2020年第三十三期
8)Mechanical force regulation of YAP by F-actin and GPCR revealed by super-resolution imaging - Nanoscale - 2020 - 2020年第四期
9)Aptamer AS1411 utilized for super-resolution imaging of nucleolin - Talanta - 2020 - 2020年第一期
10)Measurement of mechanical properties of naked cell membranes using atomic force microscope puncture test - Talanta - 2020 - 2020年第一期
11)Development of Correlative Super-Resolution Fluorescence and Three-Dimensional Topography Imaging Microscopy - Chinese Journal of Analytical Chemistry - 2020 - 2020年第二期
12)Using an RNA aptamer probe for super-resolution imaging of native EGFR - Nanoscale Advances - 2019 - 2019年第一期
13)Super-resolution imaging of cancer-associated carbohydrates using aptamer probes - Nanoscale - 2019 - 2019年第三十一期
14)The structural characteristics of mononuclear-macrophage membrane observed by atomic force microscopy - Journal of Structural Biology - 2019 - 2019年第三期
15)Mechanistic insights into GLUT1 activation and clustering revealed by super-resolution imaging - Proceedings of the National Academy of Sciences - 2018 - 2018年第二十七期
16)Single glucose molecule transport process revealed by force tracing and molecular dynamics simulations - Nanoscale Horizons - 2018 - 2018年第五期
17)Aptamer-recognized carbohydrates on the cell membrane revealed by super-resolution microscopy - Nanoscale - 2018 - 2018年第十六期
18)The structure and function of cell membranes studied by atomic force microscopy - Seminars in cell & developmental biology - 2018 - 2018年第一期
19)The process of wrapping virus revealed by a force tracing technique and simulations - Advanced Science - 2017 - 2017年第四期
20)Clustered localization of Stat3 during the cell cycle detected by super-resolution fluorescence microscopy - Methods and Applications in Fluorescence - 2017 - 2017年第二期
21)Cell contact and pressure control of yap localization and clustering revealed by super-resolution imaging - Nanoscale - 2017 - 2017年第九期
22)Super-resolution microscopy reveals the insulin-resistance-regulated reorganization of GLUT4 on plasma membranes - Journal of Cell Science - 2017 - 2017年第一百三十期
23)Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor - Cell Research - 2017 - 2017年第二十七期
24)Full-colour carbon dots: integration of multiple emission centres into single particles - Nanoscale - 2017 - 2017年第三十五期
25)Progress in the Correlative Atomic Force Microscopy and Optical Microscopy - Sensors - 2017 - 2017年第四期
26)Variation in carbohydrates between cancer and normal cell membranes revealed by super-resolution fluorescence imaging - Advanced Science - 2016 - 2016年第三期
27)Mechanistic insights into the distribution of carbohydrate clusters on cell membranes revealed by dSTORM imaging - Nanoscale - 2016 - 2016年第八期
28)Scanning ion conductance microscopic study for cellular uptake of cationic conjugated polymer nanoparticles - Macromolecular Bioscience - 2016 - 2016年第四期
29)Specificity and mechanism of action of alpha-helical membrane-active peptides interacting with model and biological membranes by single-molecule force spectroscopy - Scientific Reports - 2016 - 2016年
30)Systemic localization of seven major types of carbohydrates on cell membranes by dSTORM imaging - Scientific Reports - 2016 - 2016年
31)Enhanced dSTORM imaging using fluorophores interacting with cucurbituril - Science China-Chemistry - 2016 - 2016年第七期
32)Super-resolution imaging in glycoscience: New developments and challenges - Journal of Innovative Optical Health Sciences - 2016 - 2016年第三期
33)Nanoscale insights into full-length prion protein aggregation on model lipid membranes - Chemical Communications - 2016 - 2016年第五十五期
34)Super-resolution imaging of STAT3 cellular clustering during nuclear transport - RSC Advances - 2016 - 2016年第五十九期
35)Electrochemical modulation of the fluorescence of cyanine dye cy5 - Electroanalysis - 2015 - 2015年第八期
36)Real-time imaging of rabies virus entry into living vero cells - Scientific Reports - 2015 - 2015年
37)The structure and function of cell membranes examined by atomic force microscopy and single-molecule force spectroscopy - Chemical Society Review - 2015 - 2015年第四十四期
38)Ultrafast tracking of a single live virion during the invagination of a cell membrane - Small - 2015 - 2015年第十一期
39)Magnetic-field-enabled resolution enhancement in super-resolution imaging - Physical Chemistry Chemical Physics - 2015 - 2015年第十期
40)Recording the dynamic endocytosis of single gold nanoparticles by AFM-based force tracing - Nanoscale - 2015 - 2015年第十七期
41)Mechanistic insights into EGFR membrane clustering revealed by super-resolution imaging - Nanoscale - 2015 - 2015年第七期
42)Revealing the carbohydrate pattern on a cell surface by super-resolution imaging - Nanoscale - 2015 - 2015年第七期
43)Studying the mechanism of CD47-SIRP alpha interactions on red blood cells by single molecule force spectroscopy - Nanoscale - 2014 - 2014年第十七期
44)Regulation of EGFR nanocluster formation by ionic protein-lipid interaction - cell research - 2014 - 2014第二十四期
45)Single-molecule-force spectroscopy study of the mechanism of interactions between TSP-1 and CD47 - Science China-Chemistry - 2014 - 2014年第十二期
46)Entry of a novel marine DNA virus, singapore grouper iridovirus, into host cells occurs via clathrin-mediated endocytosis and macropinocytosis in a pH-dependent manner - Journal of Virology - 2014 - 2014年第二十二期
47)Atomic force microscopy of asymmetric membranes from turtle erythrocytes - Molecules and cells - 2014 - 2014年第八期
48)The asymmetric membrane structure of erythrocytes from Crucian carp studied by atomic force microscopy - Chinese science bulletin - 2014 - 2014年第二十一期
49)Studying the nucleated mammalian cell membrane by single molecule approaches - PLOS one - 2014 - 2014年第五期
50)Studying the membrane structure of chicken erythrocytes by in situ atomic force microscopy - Analytical Methods - 2014 - 2014年第二十期
51)Mapping the resting and stimulated EGFR in cell membranes with topography and recognition imaging - Analytical Methods - 2014 - 2014年第十九期
52)Surface modification of graphene nanopores for protein translocation - Nanotechnology - 2013 - 2013年第四十九期
53)Development of polymeric nanoprobes with improved Lifetime Dynamic Range and Stability for intracellular oxygen sensing - Small - 2013 - 2013年第十五期
54)The asymmetrical structure of golgi apparatus membranes revealed by in situ atomic force microscope - PLOS one - 2013 - 2013年第四期
55)High-efficiency localization of Na+-K+ ATPases on the cytoplasmic side by direct stochastic optical reconstruction microscopy - Nanoscale - 2013 - 2013年第二十三期
56)Al3+-induced far-red fluorescence enhancement of conjugated polymer nanoparticles and its application in live cell imaging - Nanoscale - 2013 - 2013年第十九期
57)The force of transporting a single amino acid into the living cell measured using atomic force microscopy - Chemical Communication - 2013 - 2013年第七十四期
58)A single-molecule force spectroscopy study of the interactions between lectins and carbohydrates on cancer and normal cells - Nanoscale - 2013 - 2013年第八期
59)Detection of carbohydrates on the surface of cancer and normal cells by topography and recognition imaging - Chemical Communications - 2013 - 2013年第二十九期
60)High resolution imaging of mitochondrial membranes by in situ atomic force microscopy - RSC Advances - 2013 - 2013年第三期
61)Direct measurement and modulation of single-molecule coordinative bonding forces in a transition metal complex - Nature Communications - 2013 - 2013第四期
62)A graphene oxide based biosensor for microcystins detection by fluorescence resonance energy transfer - Biosensors Bioelectronics - 2012 - 2012年第一期
63)Electrochemical single-molecule AFM of the redox metalloenzyme copper nitrite reductase in action - Chemphyschem - 2012 - 2012年第十二期
64)Caveolae-mediated endocytosis of biocompatible gold nanoparticles in living Hela cells - Journal of Physics-Condensed Matter - 2012 - 2012年第十六期
65)Direct evidence of lipid rafts by in situ atomic force microscopy - Small - 2012 - 2012年第八期
66)The study of single anticancer peptides interacting with HeLa cell membranes by single molecule force spectroscopy - Nanoscale - 2012 - 2012年第四期
67)Single-particle tracking of hepatitis B virus-like vesicle entry into cells - Small - 2011 - 2011年第九期
68)Recording force events of single quantum-dot endocytosis - Chemical Communications - 2011 - 2011年第十二期
69)Size-dependent endocytosis of single gold nanoparticles - Chemical Communications - 2011 - 2011年第二十八期
70)Locating the Band III protein in quasi-native cell membranes - Analytical Methods - 2010 - 2010年第七期
71)Preparation of cell membranes for high resolution imaging by AFM - Ultramicroscopy - 2010 - 2010年第四期
72)Localization of Na+-K+ ATPases in quasi-native cell membranes - Nano Letters - 2009 - 2009年第十二期
73)Single-epitope recognition imaging of native chromatin - Epigenetics& Chromatin - 2008 - 2008年
74)Imaging glycosylation - Journal of the American Chemical Society - 2008 - 2008第一百三十期
75)Tetrameric structure of centromeric nucleosomes in interphase drosophila cells - PLoS Biology - 2007 - 2007第五期
76)Single-molecule recognition imaging microscopy - Proceedings of the National Academy of Sciences of - 2004 - 2004第一百零一期
77)A relocated technique of atomic force microscopy (AFM) samples and its application in molecular biology - Ultramicroscopy - 2002 - 2002年第三期
78)Deoxyribonucleic acid sample preparation in scanning probe microscope - Chinese Journal of Aanlytical Chemistry - 2001 - 2001年第十二期
79)Self-assembled monolayer of ssDNA on Au(111) substrate - Surface Science - 2001 - 2001年第一-二期
80)Imaging chromosome by a lateral force microscope - Analytical Sciences - 2000 - 2000年第十二期
81)A mica-modified quartz resonator for a quartz crystal microbalance study - Analytical Sciences - 2000 - 2000年第十期