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云南生物资源保护与利用国家重点实验室导师教师师资介绍简介-梁连铭

本站小编 Free考研考试/2021-11-06



姓名: 梁连铭
学科:微生物学/生物工程(微生物方向)
职称/人才类别:博士,副研究员,硕士生导师,云南省中青年学术和技术带头人后备人才,
云南省****青年拔尖人才
电话/传真:
电子邮件: lianglm@#ynu.edu.cn
简历:
2001.9-2005.7,云南大学生命科学学院,生物科学专业,理学学士;
2005.9-2010.6,云南大学生命科学学院,微生物学专业,理学博士;
2010.8-2016.11,云南大学生物资源保护与利用国家重点实验室,助理研究员;
2016.12-至今,云南大学生物资源保护与利用国家重点实验室,副研究员,硕士生导师;
研究领域:
通过基因组学,转录组学,生物信息学及功能基因等方法重点开展以下两个方面的研究:
1.食线虫真菌与线虫相互作用研究:食线虫真菌侵染模式线虫秀丽隐杆线虫及根结线虫的分子机制及
线虫防御真菌的免疫机制。
2.丝状真菌产孢调控:真菌产孢调控的关键基因及其功能。
获奖及荣誉:
2015年云南省自然科学奖特等奖(排名第八),项目名称:“微生物与线虫互作机制研究”;
代表论著:
论文:
1. Liang L-M, Zou C-G, Xu J, Zhang K-Q. 2019. Signal pathways involved in microbe-nematode interactions provide new insights into the biocontrol of
plant-parasiticnematodes. Phil. Trans. R. Soc. B 374(1767):**.
2. Liu L, Cao Y-R, Zhang C-C, Fan H-F, Guo Z-Y, Yang H-Y, Chen M, Han J-J, Xu J, Zhang K-Q, Liang L-M*. 2019. An Efficient Gene Disruption System for the
Nematophagous Fungus Purpureocillium lavendulum. Fungal Biology doi: 10.1016/j.funbio.2018.10.009.
3. Cao YR, He ZK, Guo Y, Yang XX, Liang L-M*. 2018. Actinorectispora metalli sp. nov., a novel actinomycete isolated from a mine and emended description
of the genus Actinorectispora. International Journal of Systematic and Evolutionary Microbiology 68(4):1023-1027.
4.Liang L-M, Gao H, Li J, Liu L, Liu Z, Zhang KQ. 2017. The Woronin body in the nematophagous fungus Arthrobotrys oligospora is essential for trap formation and efficient
pathogenesis. Fungal Biology 121:11-20.
5. Li X, Kang YQ, Luo YL, Zhang KQ, Zou CG, Liang L-M*. 2017. The NADPH oxidase AoNoxA in Arthrobotrys oligospora functions as an initial factor in the infection of
Caenorhabditis elegans. Journal of Microbiology 55(11):885.
6.Wu QY, Zhu YY, Zou CG, Kang YQ, Liang L-M*. 2016. GPH1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus
Arthrobotrys oligospora. Journal of Microbiology 54(11):768-773
7.Cao YR, Yang WQ, Dao Y, Hu B, He ZK, Liang L-M*. 2016. Kibdelosporangium metalli sp. nov., isolated from rare earth mine of Bayan Obo in China. International Journal
of Systematic and Evolutionary Microbiology 67(1).
8. Liang L-M, Liu Z, Liu L, Li J, Gao H, Yang J, Zhang KQ. 2016. The nitrate assimilation pathway is involved in the trap formation of Arthrobotrys oligospora, a nematode
-trapping fungus. Fungal Genetics and Biology 92(33-39.
9.Liang L-M, Shen R, Mo Y, Yang J, Ji X, Zhang K-Q. 2015. A proposed adhesin AoMad1 helps nematode-trapping fungus Arthrobotrys oligospora recognizing host signals
for life-style switching. Fungal Genetics and Biology 81(172-181.
10.Liang L-M, Wu H, Liu Z, Shen R, Gao H, Yang J, Zhang K. 2013. Proteomic and transcriptional analyses of Arthrobotrys oligospora cell wall related proteins reveal
complexity of fungal virulence against nematodes. Applied Microbiology and Biotechnology 97(19):8683-8692.
11.Liang L-M, Yang J, Li J, Mo Y, Li L, Zhao X, Zhang KQ. 2011. Cloning and homology modeling of a serine protease gene (PrC) from the nematophagous fungus
Clonostachys rosea. Ann. Microbiol. 61:511-516.
12. Liang L-M, Liu S, Yang J, Meng Z, Lei L, Zhang K. 2011. Comparison of homology models and crystal structures of cuticle-degrading proteases from nematophagous fungi:
structural basis of nematicidal activity. FASEB Journal 25(6):1894-1902.
13. Liang L-M, Zhang K-Q, Liu S. 2011. Role of disulfide bonds in structural stability and flexibility of cuticle-degrading proteases from nematophagous fungi - a molecular
dynamics simulation study. Journal of Biomolecular Structure and Dynamics 28:994-995.
14.Liang L-M, Meng Z, Ye F, Yang J, Liu S, Sun Y, Guo Y, Mi Q, Huang X, Zou C, Rao Z, Lou Z, Zhang KQ. 2010. The crystal structures of two cuticle-degrading proteases
from nematophagous fungi and their contribution to infection against nematodes. FASEB Journal 24(5):1391-1400.
专著:
1. 梁连铭, 杨金奎, 张克勤. 真菌侵染线虫的分子机制. 余永年, 卯晓岚主编, 中国菌物学100年. 科学出版社. 2015. pp506–520.
2.梁连铭. 利用细菌CRISPR/Cas系统敲除酿酒酵母的基因. 李颖,李明春主编, 真菌生物学实验教程. 科学出版社. 2014. pp136–141.
3.Liang Lianming, Zou Chenggang and Zhang Ke-Qin. Future study. In: Ke-Qin Zhang and Kevin D. Hyde (ed.) Nematode-trapping fungi. Springer. 2014,
pp 377–382.
4.Liu Shuqun?, Liang Lianming?, Tao Yan, Yang LQ, Ji Xinglai, Yang Jinkui, Fu Yunxin and Zhang Ke-Qin. Structural and dynamic basis of serine proteases
fromnematophagous fungi for cuticle degradation. In: Stoytcheva M. (ed.) Pesticides in the modern world - Pests control and pesticides exposure and toxicity
assessment. Intech. 2011, pp 333–346. (?并列第一作者)
专利:
1.梁连铭,杨金奎,莫媛媛,张克勤。具有杀线虫功能的寡孢节丛孢基因工程菌株及其应用,发明专利,专利授权号:ZL4.2
2.梁连铭,刘志恒,张克勤。一种提高寡孢节丛孢捕食器官数量的方法,发明专利,专利授权号:ZL1.4

承担科研项目情况:
1. 国家自然科学基金地区项目“紫色紫孢菌营养胁迫诱导产孢的表观遗传机制研究”,项目负责人。起止时间:2019.01-2022.12,课题编号:**。
2.国家自然科学基金面上基金项目“BrlA抑制食线虫真菌紫色紫孢菌营养菌丝生长机理研究”,项目负责人。起止时间:2018.01-2019.12,
课题编号:**。
3.国家自然科学基金面上基金项目“N-乙酰葡糖胺抑制淡紫拟青霉产孢的分子机制”,项目负责人。起止时间:2016.01-2019.12,课题编号:**。
4.国家自然科学基金青年基金项目“捕食线虫真菌寡孢节丛孢细胞壁蛋白AOMad1调控致病性的分子基础”(已结题),项目负责人。
起止时间:2013.01-2015.12,课题编号:**。
5.云南省应用基础研究计划“淡紫拟青霉产分生孢子核心调控通路的功能鉴定”,项目负责人。起止时间:2016.10-2019.9,课题编号:2016FB062。
6.云南省应用基础研究计划“食线虫真菌寡孢节丛孢细胞壁比较蛋白质组研究”(已结题),项目负责人。起止时间:2011.10-2014.9,
课题编号:2011FB002。

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