\r李 佳，张崇越，郑淑贤，亓 剑，付志明\r
\r
AuthorsHTML:\r李 佳，张崇越，郑淑贤，亓 剑，付志明\r
\r
AuthorsListE:\rLi Jia，Zhang Chongyue，Zheng Shuxian，Qi Jian，Fu Zhiming\r
\r
AuthorsHTMLE:\rLi Jia，Zhang Chongyue，Zheng Shuxian，Qi Jian，Fu Zhiming\r
\r
Unit:\r天津大学装备设计与制造技术天津市重点实验室，天津 300350\r
\r
Unit_EngLish:\rTianjin Key Laboratory of Equipment Design and Manufacturing Technology，Tianjin University，Tianjin 300350，China\r
\r
Abstract_Chinese:\r为了解决颅骨组织工程支架中存在的中心处细胞存活率低的问题，针对具有血管嵌入的颅骨复合支架研制动态灌注培养装置，为多孔支架内部细胞提供营养物质以提高细胞存活率，使复合支架达到体内整合的要求．基于颅骨复合支架结构特点，设计双通道循环分别对多孔支架和血管进行灌注，为颅骨复合支架中成骨细胞和内皮细胞提供物质传输，气体循环为循环培养液提供氧气、二氧化碳，保证了培养液氧气充足和pH 稳定；模拟人体自然颅骨组织中机械刺激，建立多孔支架、血管流体模型，根据灌注室和血管入口流量计算流体所需的剪切应力，据此确定流体流量和压力的控制范围与精度，为复合支架提供合适的流量；设计三因素三水平正交试验对灌注室的结构尺寸进行优选，确定关键部位灌注室的结构，通过硅胶管连接灌注室、储液瓶、蠕动泵形成循环系统，实现动态灌注装置的总体结构安装；建立流体仿真分析有限元模型，分析不同流速的流体培养液分别对颅骨多孔支架和血管结构的影响，结果表明灌注室和血管结构内流体速度、剪切应力、压力分布均匀，满足骨组织和内皮细胞的培养生理环境，验证了装置设计的合理性．该装置可用于骨组织体外细胞的动态培养，为骨组织工程支架体外构建及血管化奠定了基础．\r
\r
Abstract_English:\rTo solve the problem of low survival rate of cells at the center of a scaffold，a dynamic perfusion culture device is developed for skull composite scaffold with embedded vessels. The device improves cell survival rate and makes composite scaffolds meet the integration requirement in vivo. Based on the structural features of the skull composite scaffold，a double-channel perfusion circulation is designed to separately perfuse porous scaffold and vessels to provide material transmission for osteoblasts and endothelial cells.Moreover，gas circulation provides oxygen and carbon dioxide for circulating culture medium，which ensures sufficient oxygen and stable pH. To simulate the mechanical environment in a natural human skull，a porous scaffold and a vascular fluid model are established，and the shear stress is calculated according to the inlet flow rate of perfusion chamber and vessel. Therefore，the control range and accuracy of fluid flow and pressure are determined，and the suitable flow rate for composite scaffolds are obtained. An orthogonal test with three factors and three levels is designed to optimize the size and determine the key parts of the perfusion chamber structure. The whole structure of the dynamic perfusion device is installed by connecting the perfusion chamber，the liquid storage bottle，and the peristaltic pump with a silica gel tube to form a circulating system. A finite element fluid analysis model is established to analyze the effect of flow with different velocities on composite scaffold. The results show that the fluid velocity，shear stress，and pressure are uniformly distributed in the perfusion chamber and vessel，and the physiological environment of bone tissue and endothelial cells is satisfactorily simulated；therefore，the rationality of the device is verified. The device can be used for dynamic cell culture of bone tissue in vitro. It lays a foundation for the construction and vascularization of bone tissue-engineered scaffolds.\r
\r
Keyword_Chinese:颅骨；组织工程；复合支架；动态灌注；流体模型\r

Keywords_English:skull；tissue engineering；composite scaffold；dynamic perfusion；fluid model\r


PDF全文下载地址:http://xbzrb.tju.edu.cn/#/digest?ArticleID=6224