\r姚芳莲，田欣露，孙 达\r
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AuthorsHTML:\r姚芳莲，田欣露，孙 达\r
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AuthorsListE:\rYao Fanglian，Tian Xinlu，Sun Da\r
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AuthorsHTMLE:\rYao Fanglian，Tian Xinlu，Sun Da\r
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Unit:\r天津大学化工学院，天津 300350\r
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Unit_EngLish:\rSchool of Chemical Engineering and Technology，Tianjin University，Tianjin 300350，China\r
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Abstract_Chinese:\r以叠氮基团的光耦合反应为交联反应制备了叠氮化羧甲基壳聚糖（AZ-CMCS）水凝胶。利用FT-IR 红外光谱、1H-NMR 核磁共振谱表征了AZ-CMCS 的结构，使用紫外可见分光光度计和流变仪跟踪了AZ-CMCS 凝胶的光交联过程，并验证了凝胶的可注射性. 使用水接触角测试仪表征了凝胶的亲疏水性. 以牛血清白蛋白（BSA）为模型药物研究了水凝胶在不同pH 值下的药物释放性能，并研究了人胚胎肺成纤维细胞（MRC-5）在水凝胶上的生长情况．结果表明：通过紫外光照射可快速得到AZ-CMCS 水凝胶，反应条件温和，易于控制；AZ-CMCS 水凝胶具有较好的可注射性，且具有一定的pH 响应性，其能够实现包载药物和药物控释的功能；此外AZ-CMCS 水凝胶对细胞无毒，可作为细胞支架，具有较好的应用前景.\r
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Abstract_English:\rThe azide carboxymethyl chitosan(AZ-CMCS) hydrogel was prepared by UV-irradiation-induced photocrosslinking reaction which was based on the optical coupling reaction of azide groups. The chemical structure of the AZ-CMCS hydrogel was characterized by FT-IR and 1H-NMR. The photo-crosslinking process was tracked by a rheometer and a UV-Vis spectrophotometer. The injectable property of the AZ-CMCS hydrogel was verified by a rheometer. The hydrophobicity of the AZ-CMCS hydrogel was assessed by a water contact tester. Bovine serum albumin
was used as the model drug to analyze the drug release property of the AZ-CMCS hydrogel at different pH values. The growth condition of human embryonic lung fibroblast (MRC-5) cells on AZ-CMCS scaffolds was also investigated. Results showed that the AZ-CMCS hydrogel can be rapidly prepared by UV irradiation. The reaction conditions are mild and controllable. The AZ-CMCS hydrogel has good injectable properties and pH sensibility. The AZ-CMCS hydrogel can load the model drug and control the drug release behavior. Moreover，the AZ-CMCS hydrogel is noncyto toxic and has a good application prospect as cell scaffold.\r
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Keyword_Chinese:光交联；羧甲基壳聚糖；水凝胶；可注射；蛋白释放；细胞支架\r

Keywords_English:photo-crosslinking；carboxymethyl chitsosan；hydrogel；injectable；protein release；cell scaffold\r


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