Zhang Yan——Professor
Nationality:
CHINA

Phone:
，**（Cell）

Email:
yan.zhang@tju.edu.cn

Office:
Office 417-4, Building 24, Tianjin University

School:
School of Pharmaceutical Science and Technology

ResearcherID:
K-1638-2015

Group weblink

zhangyanlab.org






Education Experience
2001-2006 Ph. D. School of Medicine University of Pennsylvania, PA, USA
1992-1996 Bachelor of Science Biology Nanjing University

Professional Experience
2008-2013 Research Scientist, Postdoctoral Fellow Massachusetts Institute of Technology
2013-2014 Senior Scientist R&D Systems Inc
2014-2018 Associate Professor Tianjin University
2018-2018 Professor Tianjin University

Research Area
The Zhang lab identifies and characterizes new enzymes and new metabolic pathways in nature using a combination of bioinformatics, genetic, biochemical and biophysical methods. In particular, the Zhang lab has a long term interest in metal trafficking, metalloenzymes. and their catalytic mechanisms. Other projects in the Zhang lab include synthetic biology, and immuno-based human disease diagnosis.


For more information about the Zhang Lab, please visit
http://zhangyanlab.org



Honors and Awards
Leading Talent Award in Science and Technology of Tianjin City, 2019
Peiyang Scholar, 2014
National Research Service Award 2012-2013

Patents
一种高产檀香油的重组酵母菌及其构建方法与应用( A genetically engineered baker yeast strain and its construction for high yield biosynthetic santalol oil production) Pending
催化谷氨酰胺合成靛蓝获得蓝色花卉的转基因方法 (A method to obtain transgenic blue rose by introducing non-ribosomal peptide synthetase catalyzing the formation of indigoidine from glutamine) Pending
铁和α酮戊二酸依赖性双加氧酶的酶活检测体系和方法（A generally applicable iron and α-oxoglutarate dependent dioxygenase enzyme assay system and assay kit）Patents No. ZL 2015 1 **.5

Highlighted Publications
Zhu D#, Wei Y#, Yin J, Liu D, Ang EL, Zhao H*, Zhang Y*. A pathway for degradation of uracil to acetyl coenzyme A in Bacillus megaterium. Appl & Environ Microbiol. 2020, Accepted. DOI: 10.1128/AEM.02837-19
Yin J#, Wei Y#, Liu D, Hu Y, Lu Q, Ang EL, Zhao H*, Zhang Y*?An extended bacterial reductive pyrimidine degradation pathway that enables nitrogen release from β-alanine. J Biol Chem. 2019 Aug 27. pii: jbc.RA119.010406. doi: 10.1074/jbc.RA119.010406.
Xing M#, Wei Y#, Hua G, Li M, Nanjaraj Urs AN, Wang F, Hu Y, Zhai W, Liu Y, Ang EL, Zhao H*, Zhang Y*. A gene cluster for taurine sulfur assimilation in an anaerobic human gut bacteria. Biochem J. 2019 Jul 26. pii: BCJ**. doi: 10.1042/BCJ**.
Zhou Y#, Wei Y#, Nanjaraj A.N., Lin L, Xu T, Hu Y, Ang EL, Zhao H*, Yuchi Z*, Zhang Y*. Identification and characterization of a new sulfoacetaldehyde reductase from the human gut bacterium Bifidobacterium kashiwanohense. Bioscience Rep. May 23, 2019; DOI: 10.1042/BSR**.
Tong Y#, Wei Y#, Hu Y, Ang EL, Zhao H*, Zhang Y* A pathway for isethionate dissimilation in Bacillus krulwichiae. Appl & Environ Microbiol. 2019 May. DOI: 10.1128/AEM.00793-19.
Li M#, Wei Y#, Yin J, Lin L, Zhou Y, Hua G, Cao P, Ang EL, Zhao H*, Yuchi Z*, Zhang Y*. Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense. Biochem J. 2019 May 14. doi: 10.1042/BCJ**.
Xing M#, Wei Y#, Zhou Y, Zhang J, Lin L, Hu Y, Hua G, Nanjaraj A.N.,Liu D, Wang F, Guo C, Tong Y, Li M, Liu Y, Ang E, Zhao H*, Yuchi Z*, Zhang Y*. Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria. Nat. Comm. DOI: 10.1038/s41467-019-09618-8.
Zhang J#, Nanjaraj A.N. #, Lin L, Yuchi Z*, Zhang Y*. Structure of glycerol dehydrogenase (GldA) from Escherichia coli. ACTA CRYSTALLOGR F.2019, Mar ; DOI: 10.1107/S**X**
Zhou Y#, Wei Y#, Lin L, Xu T, Ang E, Zhao H*, Yuchi Z*, Zhang Y*. Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytoca. Biochem J. 2019 ,Feb; DOI:10.1042/BCJ**.
Nanjaraj A.N., Hu Y, Li P, Yuchi Z, Chen Y*, Zhang Y*. Cloning and expression of a non-ribosomal peptide synthetase to generate blue rose. ACS Synth. Biol. DOI: 10.1021/acssynbio.8b00187
Liu D#, Wei Y#, Liu X, Zhou Y, Jiang L, Yin J, Wang F, Hu Y, Nanjaraj A.N., Liu Y, Ang E, Zhao S*, Zhao H*, Zhang Y*. Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole. Nat. Comm. 2018 Oct 11;9(1):4224. doi: 10.1038/s41467-018-06627-x.
Guo C#, Hu Y#, Yang C, Nanjaraj Urs AN, Zhang Y* Developing a colorimetric assay for Fe(II)/2-oxoglutarate-dependent dioxygenase. Anal Biochem. 2018 May 1;548:109-114.
Hua G#, Hu Y#, Yang C, Liu D, Mao Z, Zhang L, Zhang Y* Characterization of santalene synthases using an inorganic pyrophosphatase coupled colorimetric assay. Anal Biochem. 2018 Apr 15;547:26-36.
Zhang Y, Yang C, Dancis A, Nakamaru-Ogiso E. EPR studies of wild type and mutant Dre2 identify essential [2Fe–2S] and [4Fe–4S] clusters and their cysteine ligands. J Biochem. 2017 Jan;161(1):67-78.
Zhang Y#, Li H#, Zhang C, An X, Liu L, Stubbe J, Huang M. The Conserved Electron Donor Complex Dre2-Tah18 Is Required for Ribonucleotide Reductase Metallocofactor Assembly and DNA Synthesis. Proc Natl Acad Sci U S A. 2014 Apr 29; 111(17): E1695-704.
Yoon H, Knight SA, Pandey A, Pain J, Zhang Y, Pain D, Dancis A. Frataxin-bypassing Isu1: characterization of the bypass activity in cells and mitochondria. Biochem J. 2014 Apr 1;459(1):71-81.
Zhang Y, Liu L, Stubbe J. Huang M. Investigation of in vivo roles of the carboxyl terminal tails of the small subunit (ββ’) of S. cerevisiae ribonucleotide reductase: contribution to cofactor formation and inter-subunit association within the active holoenzyme. J Biol Chem. 2013 May 17;288(20):13951-9.Selected as the “Papers of The Week”， highlighted at http://www.jbc.org/content/288/20/13960/suppl/DCAuthor_profile
Zhang Y, Liu L, Wu X, An X, Stubbe J , Huang M. Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2: requirement of Rnr4 and contribution of Grx3/4 and Dre2. J Biol Chem. 2011. Dec 2;286(48):41499-509.Selected as the “Papers of The Week”, highlighted at http://www.jbc.org/content/286/48/41499/suppl/DCAuthor_profilehttp://www.asbmb.org/asbmbtoday/asbmbtoday_article.aspx?id=14939
Zhang Y, Stubbe J, Bacillus subtilis Class Ib Ribonucleotide Reductase Is a Dimanganese(III)-Tyrosyl Radical Enzyme. Biochemistry. 2011 Jun 28;50(25):5615-23.
Mühlenhoff U, Molik S, Godoy JR, Uzarska MA, Richter N, Seubert A, Zhang Y, Stubbe J, Pierrel F, Herrero E, Lillig CH, Lill R. Cytosolic monothiol glutaredoxins function in intracellular iron sensing and trafficking via their bound iron-sulfur cluster. Cell Metab. 2010 Oct 6;12(4):373-85.
Yoon H, Zhang Y, Pain J, Lyver ER, Lesuisse E, Pain D, Dancis A. Rim2, pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria. Biochem J. 2011 Nov 15;440(1):137-46.
Zhang Y, Lyver, ER, Amutha B, Ohnishi, T, Pain, D, and Dancis, A. Dre2, a conserved eukaryotic protein with both [2Fe-2S] and [4Fe-4S] clusters, functions in cytosolic iron-sulfur protein biogenesis. Mol Cell Biol. 2008 Sept. 28 (18) 5569-82.
Zhang Y, Lyver, ER, Knight, SAB, Pain, D, Lesuisse, E, and Dancis, A. Mrs3p, Mrs4p, and frataxin provide iron for Fe-S cluster synthesis in mitochondria. J Biol Chem. 2006 Aug 11;281(32):22493-502.
Zhang Y, Lyver, ER, Knight, SAB, Lesuisse, E, and Dancis, A. Frataxin and mitochondrial carrier proteins, Mrs3p and Mrs4p, cooperate in providing iron for heme synthesis. J Biol Chem. 2005 May 20; 280 (20):19794-807.
He Y, Alam SL, Proteasa SV, Zhang Y, Lesuisse E, Dancis A, and Stemmler T.L. Yeast frataxin solution structure, iron binding, and ferrochelatase interaction. Biochemistry. 2004 Dec 28;43(51):16254-62.
Deshmukh M, May M, Zhang Y, Gabbert KK, Karberg KA, Kranz RG, and Daldal F. Overexpression of ccl1-2 can bypass the need for the putative apocytochrome chaperone CycH during the biogenesis of c-type cytochromes. Mol. Microbiology 2002 Nov 46(4)1069-1080.

ResearcherID Publications
Year Title Author(s) Source Volume
2004 Yeast frataxin solution structure, iron binding, and ferrochelatase interaction He, YN; Alam, SL; Proteasa, SV; et al. Biochemistry 43
2005 Frataxin and mitochondrial carrier proteins, Mrs3p and Mrs4p, cooperate in providing iron for heme synthesis Zhang, Y; Lyver, ER; Knight, SAB; et al. Journal of Biological Chemistry 280
2006 Mrs3p, Mrs4p, and frataxin provide iron for Fe-S cluster synthesis in mitochondria Zhang, Yan; Lyver, Elise R.; Knight, Simon A. B.; et al. Journal of Biological Chemistry 281
2008 Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis Zhang, Yan; Lyver, Elise R.; Nakamaru-Ogiso, Eiko; et al. Molecular and Cellular Biology 28
2010 Cytosolic Monothiol Glutaredoxins Function in Intracellular Iron Sensing and Trafficking via Their Bound Iron-Sulfur Cluster Muehlenhoff, Ulrich; Molik, Sabine; Godoy, Jose R.; et al. Cell Metabolism 12
2011 Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria Yoon, Heeyong; Zhang, Yan; Pain, Jayashree; et al. Biochemical Journal 440
2011 Bacillus subtilis Class Ib Ribonucleotide Reductase Is a Dimanganese(III)-Tyrosyl Radical Enzyme Zhang, Yan; Stubbe, JoAnne Biochemistry 50
2011 Investigation of in Vivo Diferric Tyrosyl Radical Formation in Saccharomyces cerevisiae Rnr2 Protein REQUIREMENT OF Rnr4 AND CONTRIBUTION OF Grx3/4 AND Dre2 PROTEINS Zhang, Yan; Liu, Lili; Wu, Xiaorong; et al. Journal of Biological Chemistry 286
2013 Investigation of in Vivo Roles of the C-terminal Tails of the Small Subunit (beta beta ’) of Saccharomyces cerevisiae Ribonucleotide Reductase CONTRIBUTION TO COFACTOR FORMATION AND INTERSUBUNIT ASSOCIATION WITHIN THE ACTIVE HOLOENZYME Zhang, Yan; An, Xiuxiang; Stubbe, JoAnne; et al. Journal of Biological Chemistry 288
2014 Frataxin-bypassing Isu1: characterization of the bypass activity in cells and mitochondria Yoon, Heeyong; Knight, Simon A. B.; Pandey, Alok; et al. Biochemical Journal 459