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西北农林科技大学动物科技学院导师教师师资介绍简介-徐坤

本站小编 Free考研考试/2021-07-07


1.基本信息
徐坤,男,1985年07月生,河南省周口市淮阳县人,遗传学博士、西北农林科技大学副教授,硕士生导师。中国遗传学会会员,中国农业生物技术学会动物生物技术分会会员,西北农林科技大学博士后联谊会第三届理事会理事长(2016.01-2017.12), Frontiers in Genetics杂志客座编辑。先后应Frontiers in Genetics杂志邀请进行了Precise Genome Editing Techniques and Application和Opportunities and Challenges for Mitochondrial Genome Editing专题组稿。
2. 研究方向
动物生物技术,主要从事动物基因编辑技术和口服酵母营养免疫调控技术的开发及应用研究,招生专业动物遗传育种与繁殖和动物营养与饲料科学。
3. 承担课程
承担本科生《分子生物学(含实验)》、《动物生物化学实验》和研究生《动物基因工程技术》、《动物繁殖理论与生物技术》、《动物遗传育种进展》、《现代动物育种学》等课程教学任务。2018年陕西首届高校课堂教学创新大赛三等奖,2018年西北农林科技大学青年教师讲课比赛三等奖。
4. 学术成果
累计发表研究论著44篇,其中SCI期刊论文29篇,丛书章节论文2篇;以第一/通讯作者发表SCI论文18篇,累计影响因子73.889(发表当年)。申请发明专利9项,其中已授权6项。近五年,先后主持转基因生物新品种培育重点课题子任务、国家自然科学基金、陕西省自然科学基金、中国博士后基金等科研项目8项。代表性成果如下:
4.1主要科研项目
[1] 转基因生物新品种培育重大专项(重点课题):CRISPR/Cas9介导的新型双等位无筛选标记转基因技术(2018ZX**B)--双等位打靶及“无缝编辑”技术平台的建立子任务,201万元(37万元),2018.01-2020.12,在研,子任务负责人。
[2] 国家自然科学基金青年项目:CRISPR/Cas9介导HDR-SSA两步法猪IGF2基因“无缝编辑”新技术研究(**),25万元,2018.01-2020.12,在研,主持。
[3] 中国博士后科学基金特等资助:羊绒生长相关lncRNA基因双等位无筛选标记敲除研究(2018T111111),15万元,2018.01-2019.12,结题,主持。
[4] 陕西省自然科学基础研究计划青年项目:基因组精确编辑技术中不同形式供体DNA的优化、比较和应用研究(2017JQ3007),5万元,2017.01-2019.12,结题,主持。
[5] 中国博士后科学基金一等资助:Cas9切口酶和多元RNA阵列介导猪IGF2基因安全高效编辑(2015M580887),8万元,2016.01-2017.12,结题,主持。
4.2 代表性学术论文(#同等贡献,*通讯作者)
(1)SCI期刊论文(动物基因编辑方向)
[1] Sun Yongsen, Yan Nana, Mu Lu, Sun Bing, Deng Jingrong, Fang Yuanyuan, Shao Simin, Yan Qiang, Han Furong, Zhang Zhiying*, Xu Kun*. sgRNA-shRNA structure mediated SNP site editing on porcine IGF2 gene by CRISPR/StCas9. Front Genet. 2019 Apr 18; 10:347. (2018-IF="3.517," 中科院生物类2区)
[2] Ren Chonghua#, Xu Kun#, David Jay Sega, Zhang Zhiying*. Strategies for the enrichment and selection of genetically modified cells. Trends Biotechnol. 2019 Jan; 37(1):56-71. (2018-IF="13.747," 中科院工程技术类1区,Top期刊)
[3] Li Xinyi, Bai Yichun, Cheng Xinzhen, Peter Girgis Tawfek Kalds, Sun Bing, Wu Yun, Lyu Huijiao, Xu Kun*, Zhang Zhiying*. Efficient SSA-mediated precise genome editing using CRISPR/Cas9. FEBS J. 2018 Sep; 285(18):3362-75. (2017-IF="4.530," 中科院生物类2区,Editor’s Choice)
[4] Shao Simin, Ren Chonghua, Liu Zhongtian, Bai Yichun, Chen Zhilong, Wei Zehui, Wang Xin, Zhang Zhiying*, Xu Kun*. Enhancing CRISPR/Cas9-mediated homology directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Int J Biochem Cell Biol. 2017 Nov; 92:43-52. (2016-IF="3.505," 中科院生物类2区)
[5] Wu Yun#, Xu Kun#, Ren Chonghua, Li Xinyi, Lv Huijiao, Han Furong, Wei Zehui, Wang Xin, Zhang Zhiying*. Enhanced CRISPR/Cas9-mediated biallelic genome targeting with dual surrogate reporter-integrated donors. FEBS Lett. 2017 Mar; 591(6):903-13. (2016-IF=3.623,Highlight Article, 发表当年:中科院生物类2区,Top期刊)
[6] Yan Qiang#, Xu Kun#, Xing Jiani, Zhang Tingting, Wang Xin, Wei Zehui, Ren Chonghua, Liu Zhongtian, Zhang Zhiying*. Multiplex CRISPR/Cas9-based genome engineering enhanced by Drosha-mediated sgRNA-shRNA. Sci Rep. 2016 Dec 12; 6:38970.(2015-IF=5.228, 发表当年:中科院综合性期刊类2区)
[7] Xu Kun, Ren Chonghua, Liu Zhongtian, Zhang Tao, Zhang Tingting, Li Duo, Wang Ling, Yan Qiang, Guo Lijun, Shen Juncen, Zhang Zhiying*. Efficient genome engineering in eukaryotes using Cas9 from Streptococcus thermophilus . Cell Mol Life Sci. 2015 Jan; 72(2):383-99. (2014-IF= 5.808,中科院生物类2区, GMD Key Scientific Article)
[8] Ren Chonghua#, Xu Kun#, Liu Zhongtian, Shen Juncen, Han Furong, Chen Zhilong, Zhang Zhiying*. Dual-reporter surrogate systems for efficient enrichment of genetically modified cells. Cell Mol Life Sci. 2015 Jul; 72(14):2763-72. (2014-IF=5.808,中科院生物类2区)
[9] Guo Lijun#, Xu Kun#, Liu Zhiyuan, Zhang Cunfang, Xin Ying, Zhang Zhiying*. Assembled the Streptococcus thermophilus CRISPR array for multiplex DNA targeting . Anal Biochem. 2015 Jun 1; 478:131-3. ( 2014-IF=2.219)
[10] Zhang Cunfang#, Xu Kun#, Hu Linyong, Wang Ling, Zhang Tingting, Ren Chonghua, Zhang Zhiying*. A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering. Biotechnol Lett. 2015 Feb; 37(2):299-305. (2014-IF= 1.591)
(2)SCI期刊论文(口服酵母免疫方向及其他)
[11] Hafiz Muhammad Zakria, Han Baoquan, Yue Feng, Mu Lu, Fang Yuanyuan, Li Xiaoyu, Xu Kun*, Zhang Zhiying*. Significant body mass increase by oral administration of a cascade of shIL21-MSTN yeast-based DNA vaccine in mice. Biomedicine & Pharmacotherapy. 2019 Oct; 118:109147. (2018-IF="3.743)
[12] Han Baoquan#, Xu Kun#,*, Liu Zhongtian, Ge Wei, Shao Simin, Li Pengcheng, Yan Nana, Li Xinyi, Zhang Zhiying*. Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus. Fish Shellfish Immunol. 2019 Jan; 84:948-54. (2018-IF="3.298," 中科院农林科学类1区,Top期刊)
[13] Luo Zhixin, Wang Shanhe, Jiao Beilei, Yuan Dan, Dai Dongmei, Wang Lixian, Xu Kun*, Wang Xin*. Gene cloning and seamless site-directed mutagenesis using single strand annealing (SSA). Appl Microbiol Biotechnol. 2018 September 12; 102 (23):10119-26. (2017-IF=3.340, 中科院工程技术类2区,Top期刊)
[14] Xu Kun, Zhang Tingting, Guo Lijun, Xin Ying, Zhang Long, Zhang Zhiying*. Alternative methods for the efficient construction of short hairpin RNA expression vectors. Anal Biochem. 2015 Jun 1; 478:23-5. (2014-IF=2.219)
[15] Zhang Zhiqiang#, Xu Kun#, Xin Ying, Zhang Zhiying*. An efficient method for multiple site-directed mutagenesis using type IIs restriction enzymes. Anal Biochem. 2015 May 1; 476:26-8. (2014-IF=2.219)
[16] Xu Kun, Zhang Tingting, Wang Ling, Zhang Cunfang, Zhang Long, Ma Lixia, Xin Ying, Ren Chonghua, Zhang Zhiqiang, Yan Qiang, Martineau Daniel, Zhang Zhiying*. Walleye dermal sarcoma virus: expression of a full-length clone or the rv-cyclin (orf a) gene is cytopathic to the host and human tumor cells. Mol Biol Rep. 2013 Feb; 40(2):1451-61. ( 2012-IF=2.506)
[17] Wang Ling#, Xu Kun#, Lin Juan, Shao Simin, Zhang Tingting, Xu Huarong, Wei Zehui, Zhang Zhiying*. A novel genetic system based on zinc finger nucleases for the identification of interactions between proteins in vivo. PLoS One. 2013 Dec 31; 8(12):e85650. (2012-IF= 3.730,发表当年:中科院生物类2区Top期刊)
(3)专著/丛书章节论文
[18] Zhang Zhiying, Segal David Jay, Xu Kun, eds (2020). Precise Genome Editing Techniques and Applications. Lausanne: Frontiers Media SA.doi: 10.3389/978-2-88963-779-9.
[19] Xu Kun, Liu Zhongtian, Zhang Long, Zhang Tingting, Zhang Zhiying. SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast. In: Shum K., Rossi J. (eds) SiRNA Delivery Methods (Chapter 14). Methods in Molecular Biology (2016), vol 1364. Humana Press, New York, NY.
[20] Xing Jiani, Zhang Cunfang, Xu Kun, Hu Linyong, Wang Ling, Zhang Tingting, Ren Chonghua, Zhang Zhiying. An Improved Genome Engineering Method Using Surrogate Reporter-Coupled Suicidal ZFNs. In: Liu J. (eds) Zinc Finger Proteins (Chapter 13). Methods in Molecular Biology (2018), vol 1867. Humana Press, New York, NY.
4.3 授权发明专利
[1] 一种增强基因表达的自激活Gal4/UAS系统表达盒的建立。徐坤;刘玉万;张智英;闫娜娜;雒志新;韩宝泉;李欣憶;张哓兰。ZL 7.1. 授权日期2020/5/19.
[2] 基于人工核酸酶的可视化细胞修复效率报告系统及其建立方法. 张智英; 邵斯旻; 徐坤; 韩芙蓉; 沈俊岑; 白义春. ZL8.6. 授权日期2020/4/21.
[3] 一种利用CRISPR/Cas9技术基于SSA修复的基因无缝编辑方法. 张智英; 白义春; 徐坤; 魏泽辉; 和林洁; 任充华; 邵斯旻; 吴芸; 刘中天. ZL2.3. 授权日期2019/10/11
[4] .一种T7 RNA聚合酶介导的CRISPR/Cas9基因编辑系统的构建方法. 张智英; 邢佳妮; 闫强; 徐坤。ZL1.3.授权日期2019/10/11.
[5] 基于CRISPR/Cas9的外源基因敲入整合系统及其建立方法和应用. 张智英; 吴芸; 徐坤; 白义春; 吕慧娇。ZL6.4. 授权日期2019/10/8.
[6] 一种口服重组酵母及其介导的靶向肠道DC呈递shRNA的应用. 张智英; 张龙; 邵斯旻; 徐坤; 李欣憶; 徐华荣。ZL9.3. 授权日期2016/4/13.
5.联系方式
通讯地址:陕西杨凌西农路22号西农大北校区动科楼225办公室
邮编:712100
联系电话:**
Email:xukunas@nwafu.edu.cn,@qq.com
(更新时间2020.07.19)



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