删除或更新信息,请邮件至freekaoyan#163.com(#换成@)

Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and

本站小编 Free考研考试/2022-02-11

Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects
Han, Yijing1,2,3,4; Zhang, Qianqian1,2,3; Yang, Dinglong1,2,3; Zhao, Jianmin1,2,3; Chen, Lizhu5; Yu, Daode6
发表期刊DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
2021-03
卷号116页码:103953
关键词Ruditapes philippinarumExtracellular trapsBactericidal effectReactive oxygen speciesGlycolysis
DOI10.1016/j.dci.2020.103953
英文摘要Extracellular traps (ETs) have been found to be an important strategy of mammals to immobilize and kill invading microorganisms. In the present study, we observed the formation of ETs in the hemocytes of marine mollusks Ruditapes philippinarum in response to challenge from bacteria Vibrio anguillarum, and examined the potential factors and signaling pathways underling this process. We detected an increase of reactive oxygen species (ROS) and myeloperoxidase (MPO) production during ETosis, accompanied by significantly up-regulated expression of ROS-related and MPO genes. The suppression of ETs structures by nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor (diphenyleneiodonium chloride, DPI) and MPO inhibitor (aminobenzoic acid hydrazide, ABAH) further confirmed the essential roles ROS and MPO played in ETosis. Furthermore, ET production could be inhibited by phosphotidylinsitol-3-kinase (PI3K) inhibitor (LY294002) and extracellular regulated protein kinase (ERK) inhibitor (U0126), suggesting the idea that both the PI3K and ERK pathways were suggested to function during ETosis. In addition, the ETosis process was accompanied by enhancement of glycolysis-related enzymatic activities, e.g., pyruvate kinase (PK) and hexokinase (HK), and over-expression of the glycolysis-related genes, e.g., PK, HK and glucose transport protein (GLUT), indicating high involvement of glycolysis in the ETosis process. Furthermore, our scanning electron microscopy (SEM) observation and antibacterial activities test successfully showed the patterns how clam ETs entrapped and killed the invading V. anguillarum. Taken together, our results revealed that ETosis with bactericidal effect increased ROS, MPO and glycolysis level and carried out in a ROS-, MPO-, PI3K-ERK-dependent manner.
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41806196]; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022]; Yantai Science and Technology Development Project [2020MSGY066]; Youth Innovation Promotion Association, Chinese Academy of Sciences [2019216]
收录类别SCI
语种英语
关键词[WOS]CAPTURE BACTERIA; DNA TRAPS; RELEASE; NEUTROPHILS; ACTIVATION; IMMUNITY; ROLES; DEATH; BURST
研究领域[WOS]Fisheries; Immunology; Veterinary Sciences; Zoology
WOS记录号WOS:000607045500001
引用统计被引频次:1[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30517
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心

作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China;
6.Marine Biol Inst Shandong Prov, Qingdao 266002, Shandong, Peoples R China

推荐引用方式
GB/T 7714Han, Yijing,Zhang, Qianqian,Yang, Dinglong,et al. Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,116:103953.
APAHan, Yijing,Zhang, Qianqian,Yang, Dinglong,Zhao, Jianmin,Chen, Lizhu,&Yu, Daode.(2021).Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,116,103953.
MLAHan, Yijing,et al."Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 116(2021):103953.


PDF全文下载地址:

点我下载PDF
相关话题/海岸 生物学 文献 推荐 英语

  • 领限时大额优惠券,享本站正版考研考试资料!
    大额优惠券
    优惠券领取后72小时内有效,10万种最新考研考试考证类电子打印资料任你选。涵盖全国500余所院校考研专业课、200多种职业资格考试、1100多种经典教材,产品类型包含电子书、题库、全套资料以及视频,无论您是考研复习、考证刷题,还是考前冲刺等,不同类型的产品可满足您学习上的不同需求。 ...
    本站小编 Free壹佰分学习网 2022-09-19
  • 2000—2010年亚欧大陆中低纬度海岸带土地利用/覆盖变化及驱动力分析
    2000—2010年亚欧大陆中低纬度海岸带土地利用/覆盖变化及驱动力分析侯婉1,2,3,4,5;侯西勇1,4,5;孙敏1,4,5;宋百媛1,3,4,5发表期刊世界地理研究ISSN1004-94792021卷号30期号:4页码:813-825关键词土地利用/覆盖变化驱动力分析多源数据融合亚欧大陆中低纬 ...
    本站小编 Free考研考试 2022-02-11
  • 基于海岸带成像仪CZI的连云港紫菜养殖动态监测
    基于海岸带成像仪CZI的连云港紫菜养殖动态监测王鑫华1,2;刘建强3;邢前国1,2;陈艳拢4发表期刊海洋科学ISSN1000-30962021卷号45期号:7页码:9-17关键词HY-1C海岸带成像仪(CZI)NDVI连云港紫菜养殖中文摘要连云港海域的紫菜养殖遥感监测对于规划紫菜养殖空间分布具有重要 ...
    本站小编 Free考研考试 2022-02-11
  • 烟台海岸带微塑料的污染特征研究
    烟台海岸带微塑料的污染特征研究程姣姣1,2;李艳芳1;邹涛1;唐诚1发表期刊环境保护ISSN0253-97052020卷号48期号:23页码:47-52关键词微塑料海岸带环境介质养殖活动垂直运动中文摘要为揭示近海海岸带微塑料的分布特征与主要来源,本文聚焦烟台海岸带区域,对潮滩、水体、沉积物和河流等不 ...
    本站小编 Free考研考试 2022-02-11
  • 面向多目标的海岸带生态空间规划路径及案例
    面向多目标的海岸带生态空间规划路径及案例陈梦缘;袁秀;王德;邢韶华发表期刊城乡规划ISSN2096-30252021-08-15期号04页码:46-53关键词陆海统筹海岸带空间规划多目标保护中文摘要海岸带是陆海统筹综合管理实施的关键地带,其关键要素包括土地(滨海湿地)、水(淡水、咸水及废水)和生物( ...
    本站小编 Free考研考试 2022-02-11
  • 单环刺螠的分子生物学研究进展
    单环刺螠的分子生物学研究进展吕慧超;李秉钧;焦绪栋发表期刊生物学通报ISSN0006-31932020-08-20卷号55期号:08页码:1-4关键词单环刺螠分子生物学生殖发育活性物质硫代谢中文摘要单环刺螠是我国北方沿海地区常见底栖经济类无脊椎动物。由于海岸带开发导致的生境破坏,以及滥采滥捕、环境变 ...
    本站小编 Free考研考试 2022-02-11
  • 基于多源数据的中国海岸带地区人口空间化模拟
    基于多源数据的中国海岸带地区人口空间化模拟其他题名SpatialSimulationofPopulationinChina'sCoastalZonebasedonMulti-sourceData杜培培1,2,3,4;侯西勇1,3,4发表期刊地球信息科学学报ISSN1560-89992020卷号22期 ...
    本站小编 Free考研考试 2022-02-11
  • 环渤海海岸大气微塑料污染时空分布特征与表面形貌
    环渤海海岸大气微塑料污染时空分布特征与表面形貌其他题名ThetemporalandspatialdistributionandsurfacemorphologyofatmosphericmicroplasticsaroundtheBohaiSea田媛1,3;涂晨1;周倩1,3;张晨捷1,3;李连祯1 ...
    本站小编 Free考研考试 2022-02-11
  • 国际海岸线变化研究进展综述基于文献计量学方法
    国际海岸线变化研究进展综述基于文献计量学方法其他题名Abibliometricreviewoftheinternationalshorelinechanges张玉新1,2,3,4;侯西勇1,3,4发表期刊应用海洋学学报ISSN2095-49722020卷号39期号:2页码:289-301关键词海岸带 ...
    本站小编 Free考研考试 2022-02-11
  • 1987-2017年莱州湾沿岸土地利用变化及其对海岸线变化的影响
    1987-2017年莱州湾沿岸土地利用变化及其对海岸线变化的影响其他题名LandUseChangeandItsInfluenceonCoastlineChangeAlongLaizhouBayDuring1987-2017王志成1,2,3;高志强1,3;尚伟涛1,3;姜晓鹏1,3发表期刊水土保持通报 ...
    本站小编 Free考研考试 2022-02-11
  • 基于InVEST模型的庙岛群岛海岸脆弱性研究
    基于InVEST模型的庙岛群岛海岸脆弱性研究其他题名CoastalvulnerabilityofMiaodaoarchipelagobasedonInVESTmodel殷丽婷1,2;郑伟3;高猛1;路景钫4发表期刊海洋环境科学ISSN1007-63362021卷号40期号:2页码:221-227关键 ...
    本站小编 Free考研考试 2022-02-11