Chen, Hao1; Choi, Namhyun1; Moon, Joung-Il1; Dang, Hajun1; Das, Anupam1; Choo, Jaebum1; Park, Sung-Gyu2; Lee, Seunghun2; Kim, Do-Geun2; Chen, Lingxin3
发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
2020-11-01
卷号167页码:112496
关键词Surface-enhanced Raman scattering (SERS)AptasensorSERS imaging sensorInfluenza A virusSERS-based assay
DOI10.1016/j.bios.2020.112496
英文摘要Surface-enhanced Raman scattering (SERS)-based aptasensors display high sensitivity for influenza A/H1N1 virus detection but improved signal reproducibility is required. Therefore, in this study, we fabricated a three-dimensional (3D) nano-popcorn plasmonic substrate using the surface energy difference between a perfluorodecanethiol (PFDT) spacer and the Au layer. This energy difference led to Au nanoparticle self-assembly; neighboring nanoparticles then created multiple hotspots on the substrate. The localized surface plasmon effects at the hot spots dramatically enhanced the incident field. Quantitative evaluation of A/H1N1 virus was achieved using the decrease of Raman peak intensity resulting from the release of Cy3-labeled aptamer DNAs from nano-popcorn substrate surfaces via the interaction between the aptamer DNA and A/H1N1 virus. The use of a Raman imaging technique involving the fast mapping of all pixel points enabled the reproducible quantification of A/H1N1 virus on nano-popcorn substrates. Average ensemble effects obtained by averaging all randomly distributed hot spots mapped on the substrate made it possible to reliably quantify target viruses. The SERS-based imaging aptasensor platform proposed in this work overcomes the issues inherent in conventional approaches (the time-consuming and labor-intensiveness of RT-PCR and low sensitivity and quantitative analysis limits of lateral flow assay kits). Our SERS-based assay for detecting A/H1N1 virus had an estimated limit of detection of 97 PFU mL(-1) (approximately three orders of magnitude more sensitive than that determined by the enzyme-linked immunosorbent assay) and the approximate assay time was estimated to be 20 min. Thus, this approach provides an ultrasensitive, reliable platform for detecting viral pathogens.
资助机构government-wide R&D Fund for the research of infectious diseases in Korea [HG18C0062]; National Research Foundation of KoreaNational Research Foundation of Korea [2019R1A2C3004375, 2017M2A2A6A01019037]; Ministry of Science, ICT, and Future Planning [POC3390]; Fundamental Research Program of the Korean Institute of Materials Science [KIMS] [PNK 6800]; Ministry of Trade, Industry, and Energy [N0002310]
收录类别SCI
语种英语
关键词[WOS]POLYMERASE-CHAIN-REACTION; A H1N1 VIRUS; COLORIMETRIC DETECTION; SPECTROSCOPY; ACCURACY; ARRAYS; TESTS
研究领域[WOS]Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics
WOS记录号WOS:000569863100006
引用统计被引频次：43[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30499
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea;
2.Korea Inst Mat Sci KIMS, Adv Nanosurface Dept, Chang Won 51508, South Korea;
3.Chinese Acad Sci, Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Chen, Hao,Choi, Namhyun,Moon, Joung-Il,et al. SERS imaging-based aptasensor for ultrasensitive and reproducible detection of influenza virus A[J]. BIOSENSORS & BIOELECTRONICS,2020,167:112496.
APAChen, Hao.,Choi, Namhyun.,Moon, Joung-Il.,Dang, Hajun.,Das, Anupam.,...&Chen, Lingxin.(2020).SERS imaging-based aptasensor for ultrasensitive and reproducible detection of influenza virus A.BIOSENSORS & BIOELECTRONICS,167,112496.
MLAChen, Hao,et al."SERS imaging-based aptasensor for ultrasensitive and reproducible detection of influenza virus A".BIOSENSORS & BIOELECTRONICS 167(2020):112496.
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