Chen, Weiwei1,2,3; Kong, Yachao1,2,3; Li, Junde1,2,3; Sun, Yanyu1,2,3; Min, Jun1,3
; Hu, Xiaoke1,3,4; Min, Jun4发表期刊INTERNATIONAL BIODETERIORATION & BIODEGRADATION
ISSN0964-8305
2020-10
卷号154页码:105047
关键词Crude oilConsortiumBiosurfactantBioaugmentationAlkane hydroxylase genes
DOI10.1016/j.ibiod.2020.105047
英文摘要Bioremediation is an attractive strategy of utilizing bacteria to remove crude oil contaminants. In this study, two salt-tolerant crude oil-degrading and biosurfactant-producing bacteria, Dietzia sp. CN-3 and Acinetobacter sp. HC8-3S, were functionally combined to construct a bacterial consortium. The consortium achieved 95.8% degradation efficiency of crude oil in 10 days and various n-alkanes, cycloalkanes, branched alkanes and aromatic hydrocarbons were all depleted more effectively than single strains. Functional optimization of the consortium degraded crude oil efficiently in a wide range of pH (4-10) and salinity (0-120 g L-1). Furthermore, two alkane hydroxylase genes, alkB in CN-3 and alkM in HC8-3S, were cloned and their expression were examined by real-time quantitative polymerase chain reaction, indicating that alkB was more prominent in long-chain alkanes (C-20, C-24 and C-26) utilization and alkM played crucial roles in medium- and long-chain alkanes (C-14, C-16, C-20, C-24 and C-26) degradation. In soil microcosms artificially contaminated with crude oil and bioaugmented with the consortium, 58.3% of total petroleum hydrocarbons were depleted after 60 days and the degradation rate (485.8 mg kg(-1) d(-1)) was higher than those reported in previous studies. Consequently, the consortium is a promising candidate in crude oil bioremediation.
资助机构Ministry of Science and Technology of the People's Republic of ChinaMinistry of Science and Technology, China [2016YFC1402300]; Youth Innovation Promotion Association of the Chinese Academy of Sciences, China [2020218]; Yantai Science and Technology Bureau, China [2017ZH092]; Chinese Academy of Sciences, ChinaChinese Academy of Sciences [133337KYSB20180015]
收录类别SCI
语种英语
关键词[WOS]CONTAMINATED SOIL; GENE-EXPRESSION; DEGRADATION; BIOREMEDIATION; BIOAUGMENTATION; HYDROCARBONS; STRAINS; EFFICIENCY; COASTAL; ALKANES
研究领域[WOS]Biotechnology & Applied Microbiology; Environmental Sciences & Ecology
WOS记录号WOS:000576666100002
引用统计被引频次：8[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30496
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Chunhui Rd, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing, Peoples R China;
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
推荐引用方式
GB/T 7714Chen, Weiwei,Kong, Yachao,Li, Junde,et al. Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers[J]. INTERNATIONAL BIODETERIORATION & BIODEGRADATION,2020,154:105047.
APAChen, Weiwei.,Kong, Yachao.,Li, Junde.,Sun, Yanyu.,Min, Jun.,...&Min, Jun.(2020).Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers.INTERNATIONAL BIODETERIORATION & BIODEGRADATION,154,105047.
MLAChen, Weiwei,et al."Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers".INTERNATIONAL BIODETERIORATION & BIODEGRADATION 154(2020):105047.
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