Ding, Yangjun1; Shang, Hezhen2; Wang, Xiaokun3; Chen, Lingxin4,5
发表期刊ANALYST
ISSN0003-2654
2020-09-21
卷号145期号:18页码:6079-6084
关键词COLORIMETRIC APTASENSORGOLDNANOPARTICLESTOXICITYPROBESRISK
DOI10.1039/d0an01220c
英文摘要Ochratoxin A (OTA) is a well-known carcinogenic contaminant in food commodities. The highly sensitive and specific detection of the OTA level is an essential way to prevent the health risks to humans/animals. In this work, an SERS-based competitive immunoassay platform has been developed for the detection of OTA. This assay contains two compartments: OTA-BSA-immobilized SERS nanotags and anti-OTA antibody-functionalized magnetic beads. In the presence of target OTA, a competitive reaction towards magnetic beads occurs between the target OTA and SERS nanotags. The characteristic peaks of SERS nanotags adsorbed onto magnetic beads were used for the quantitative analysis of OTA. Under optimized conditions, a good linear relationship was obtained in the range of 1 pg mL(-1)to1000 pg mL(-1). The limit of detection (LOD) was estimated to be 0.61 pg mL(-1)using the IUPAC standard method. In addition, this proposed method was also successfully applied for the detection of OTA in wine samples and satisfactory recovery rates were obtained in the range of 90.60% to 103.3%. It is believed that this SERS-based competitive method holds great potential for highly sensitive and specific detection of mycotoxins.
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21904078, 21976209]; Taishan Scholar Project Special Funding [ts20190962]
收录类别SCI
语种英语
关键词[WOS]COLORIMETRIC APTASENSOR; GOLD; NANOPARTICLES; TOXICITY; PROBES; RISK
研究领域[WOS]Chemistry
WOS记录号WOS:000568961600009
引用统计被引频次：8[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30492
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China;
2.Qingdao Chengyang Dist Peoples Hosp, Dept Hepatobiliary Surg, Qingdao 266109, Peoples R China;
3.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China;
4.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Ding, Yangjun,Shang, Hezhen,Wang, Xiaokun,et al. A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A[J]. ANALYST,2020,145(18):6079-6084.
APADing, Yangjun,Shang, Hezhen,Wang, Xiaokun,&Chen, Lingxin.(2020).A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A.ANALYST,145(18),6079-6084.
MLADing, Yangjun,et al."A SERS-based competitive immunoassay for highly sensitive and specific detection of ochratoxin A".ANALYST 145.18(2020):6079-6084.
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