Wang, Kang1; Gao, Zhengquan1; Meng, Chunxiao1; Cui, Yulin2,3
; Wang, Yinchu2,3; Qin, Song2,3; Liu, Tianzhong4,5发表期刊MARINE BIOTECHNOLOGY
ISSN1436-2228
2020-08
卷号22期号:4页码:572-580
关键词Haematococcus pluvialisChloroplast transformationAntimicrobial peptideBarBiolistic method
DOI10.1007/s10126-020-09978-z
英文摘要The purpose of this study was to express an antimicrobial peptide in the chloroplast to further develop the plastid engineering ofH. pluvialis. Homologous targeting of the 16S-trnI/trnA-23S region and four endogenous regulatory elements, including thepsbApromoter,rbcLpromoter,rbcLterminator, andpsbAterminator inH. pluvialis, were performed to construct a chloroplast transformation vector forH. pluvialis. The expression of codon-optimized antimicrobial peptide piscidin-4 gene (ant1) and selection marker gene (bar, biolaphos resistance gene) in the chloroplast ofH. pluvialiswas controlled by therbcLpromoter andpsbApromoter, respectively. Upon biolistic transformation and selection with phosphinothricin, integration and expression ofant1in the chloroplast genome were detected using polymerase chain reaction (PCR), southern blotting, and western blotting. Using this method, we successfully expressed antimicrobial peptide piscidin-4 inH. pluvialis. Hence, our results showedH. pluvialispromises as a platform for expressing recombinant proteins for biotechnological applications, which will further contribute to promoting genetic engineering improvement of this strain.
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41876188, 31972815]; Major Basic Research Program of Shandong Province Natural Science Foundation [ZR2018ZB0210, ZR2019ZD17]; Project of Innovation & Development of Marine Economy [HHCL201803]; Key Research and Development Program of Shandong Province (Food for Special Medical Purpose) [2018YYSP016]; Key Research and Development Program of Shandong Province [2017CXGC0309]; Project of Shandong Province Higher Educational Science and Technology Program [J17KA132]
收录类别SCI
语种英语
关键词[WOS]HAEMATOCOCCUS-PLUVIALIS; ASTAXANTHIN ACCUMULATION; ALGA HAEMATOCOCCUS; MICROALGAE; GROWTH; PATHWAYS; PROTEINS; GENOMES
研究领域[WOS]Biotechnology & Applied Microbiology; Marine & Freshwater Biology
WOS记录号WOS:000539935200001
引用统计被引频次：4[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30482
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China;
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Microalgal Biotechnol Grp, CAS Key Lab Biofuels, Qingdao 266101, Peoples R China;
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Lab Energy Genet, Qingdao 266101, Peoples R China
推荐引用方式
GB/T 7714Wang, Kang,Gao, Zhengquan,Meng, Chunxiao,et al. Chloroplast Genetic Engineering of a Unicellular Green AlgaHaematococcus pluvialiswith Expression of an Antimicrobial Peptide[J]. MARINE BIOTECHNOLOGY,2020,22(4):572-580.
APAWang, Kang.,Gao, Zhengquan.,Meng, Chunxiao.,Cui, Yulin.,Wang, Yinchu.,...&Liu, Tianzhong.(2020).Chloroplast Genetic Engineering of a Unicellular Green AlgaHaematococcus pluvialiswith Expression of an Antimicrobial Peptide.MARINE BIOTECHNOLOGY,22(4),572-580.
MLAWang, Kang,et al."Chloroplast Genetic Engineering of a Unicellular Green AlgaHaematococcus pluvialiswith Expression of an Antimicrobial Peptide".MARINE BIOTECHNOLOGY 22.4(2020):572-580.
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