Liu, Wei1,2
; Li, Yan1,2; Bai, Xue1; Wu, Haiguang1,3; Bian, Lanxing1,3; Hu, Xiaoke1,2; Bai, Xue3发表期刊MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN0894-0282
2020-03
卷号33期号:3页码:528-538
关键词Azorhizobium caulinodansHTH domainLuxR-type regulatorREC domainRhizobium-legume symbiosis
DOI10.1094/MPMI-10-19-0306-R
英文摘要LuxR-type regulators play important roles in transcriptional regulation in bacteria and control various biological processes. A genome sequence analysis showed the existence of seven LuxR-type regulators in Azorhizobium caulinodans ORS571, an important nitrogen-fixing bacterium in both its free-living state and in symbiosis with its host, Sesbania rostrata. However, the functional mechanisms of these regulators remain unclear. In this study, we identified a LuxR-type regulator that contains a cheY-homologous receiver (REC) domain in its N terminus and designated it AclR1. Interestingly, phylogenetic analysis revealed that AclR1 exhibited relatively close evolutionary relationships with MalT/GerE/FixJ/NarL family proteins. Functional analysis of an aclR1 deletion mutant (Delta aclR1) in the free-living state showed that AclR1 positively regulated cell motility and flocculation but negatively regulated exopoly-saccharide production, biofilm formation, and second messenger cyclic diguanylate (c-di-GMP)-related gene expression. In the symbiotic state, the Delta aclR1 mutant was defective in competitive colonization and nodulation on host plants. These results suggested that AclR1 could provide bacteria with the ability to compete effectively for symbiotic nodulation. Overall, our results show that the REC-LuxR-type regulator AclR1 regulates numerous phenotypes both in the free-living state and during host plant symbiosis.
资助机构Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2016CB44]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31800099,41576165, 31600009]
收录类别SCI
语种英语
关键词[WOS]PROTEIN; SYSTEM; NODULATION
研究领域[WOS]Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences
WOS记录号WOS:000551075100015
引用统计被引频次：4[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30465
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;
3.Yantai Univ, Coll Life Sci, Yantai, Peoples R China
推荐引用方式
GB/T 7714Liu, Wei,Li, Yan,Bai, Xue,et al. LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2020,33(3):528-538.
APALiu, Wei.,Li, Yan.,Bai, Xue.,Wu, Haiguang.,Bian, Lanxing.,...&Bai, Xue.(2020).LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States.MOLECULAR PLANT-MICROBE INTERACTIONS,33(3),528-538.
MLALiu, Wei,et al."LuxR-Type Regulator AclR1 of Azorhizobium caulinodans Regulates Cyclic di-GMP and Numerous Phenotypes in Free-Living and Symbiotic States".MOLECULAR PLANT-MICROBE INTERACTIONS 33.3(2020):528-538.
PDF全文下载地址:
点我下载PDF
