其他题名Environmentally friendly ratiometric fluorescent microfluidic paper chip for rapid detection of difenoconazole
马昕1; 郝帼英1; 张忠1,2; 李金花2,3
; 杨兴斌1; 陈令新2,3发表期刊中国科学. 化学
ISSN1674-7224
2020
卷号50期号:3页码:393-405
关键词分子印迹碳量子点比率荧光苯醚甲环唑微流控纸芯片
研究领域Chemistry
英文摘要A novel ratiometric fluorescent imprinted microfluidic device was constructed to rapidly detect trace pesticide residues.NBD-APTES was used as control fluorescence, and the fluorescence of carbon quantum dots modified with cysteine(CDs-Cys)was constructed to achieve rapid visual detection of the bacteriostatic agent difenoconazole.The analytical performance of paper-based chips was studied in detail using scanning electron microscopy and laser confocal microscopy.Under optimized conditions, the linear range of the fluorescence sensor is 0.3-60 mumol/L, the LOD is 75 nmol/L, the recoveries are 102.1%-111.2%, and the accuracy is 3.1%-4.2%.Compared with the traditional liquid phase fluorescence sensing materials, the ratiometric fluorescence sensor under the solid phase matrix has better portability and storability, showing satisfactory fluorescence characteristics.In addition, the sensor was highly specific for separating and detecting difenoconazole and was successfully applied to actual sample.It opens up new ways for the combination of ratiometric fluorescence technology and paper-based microfluidic chip, and provides potential point-of-care applications.
中文摘要本文构建了一种新型比率荧光印迹微流控纸芯片,将绿色荧光(NBD-APTES)作为对照荧光源,以半胱氨酸修饰后的碳量子点(CDs-Cys)的荧光变化来实现苯醚甲环唑的快速可视化检测.采用扫描电子显微镜和激光共聚焦显微镜等详细研究纸基芯片的检测性能.在优化条件下,该荧光传感器的线性范围为0.3~60 mumol/L,检测限为75 nmol/L,样品回收率为102.1%~111.2%,准确度在3.1%~4.2%.与传统液相荧光传感材料相比,固相基质的比率荧光传感器具备更好的携带性和储存性,表现出令人满意的荧光检测特性.此外,该传感器用于分离和检测苯醚甲环唑时具有高度的特异性,已成功地应用于实际样品的检测,为新型比率荧光技术与微流控纸基芯片结合开辟了新途径,并为未来提供了潜在的即时医疗应用前景.
文章类型Article
资助机构国家自然科学基金; 香江****计划; 中国科学院海岸带环境过程与生态修复重点实验室开放基金; 泰山****工程专项经费
收录类别CSCD
语种中文
CSCD记录号CSCD:6691081
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30370
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.陕西师范大学食品工程与营养科学学院,陕西省食品绿色加工与安全控制工程实验室,西安710062;
2.中国科学院海岸带环境过程与生态修复重点实验室,中国科学院烟台海岸带研究所,烟台264003;
3.中国科学院海洋大科学研究中心,青岛266071
推荐引用方式
GB/T 7714马昕,郝帼英,张忠,等. 环境友好型比率荧光微流控纸芯片快速检测苯醚甲环唑[J]. 中国科学. 化学,2020,50(3):393-405.
APA马昕,郝帼英,张忠,李金花,杨兴斌,&陈令新.(2020).环境友好型比率荧光微流控纸芯片快速检测苯醚甲环唑.中国科学. 化学,50(3),393-405.
MLA马昕,et al."环境友好型比率荧光微流控纸芯片快速检测苯醚甲环唑".中国科学. 化学 50.3(2020):393-405.
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