其他题名Advances in polychlorinated biphenyls degrading bacteria of Burkholderia xenovorans LB400
孙成成1,2,3; 李俊德1,2,3; 闵军1,2
; 胡晓珂1,2,3发表期刊微生物学通报
ISSN0253-2654
2020
卷号47期号:10页码:3393-3406
关键词多氯联苯(PCBs)代谢途径降解酶
研究领域Environmental Sciences & Ecology
英文摘要Burkholderia xenovorans LB400 is a strain of polychlorinated biphenyls degrading bacteria PCBs. This organism also cooxidizes chlorinated biphenyl (CB) congeners containing from one to six chlorine substituents. In recent years, LB400 has become a model organism to study the biochemistry and molecular biology of CB metabolism in prokaryotes, because of excellent degradability and a broad spectrum of CB congeners. At present, the research on strain LB400 is mainly focused on degradation genes, enzymes and enzymes evolution, while its degradation pathway, substrate range and related mechanism of PCBs have been widely discussed; Furthermore, there are few studies on regulatory genes related to degradation of PCBs. This paper focuses on the degradation of polychlorinated biphenyls, and describes its metabolic pathway, related genes and enzyme system and artificial evolution. We hope to provide a reference for the further study of the application of Burkholderia xenovorans LB400 and other polychlorinated biphenyls degradation strains.
中文摘要Burkholderia xenovorans LB400是一株多氯联苯(polychlorinated biphenyls,PCBs)降解菌,可以氧化含有1-6个氯取代基的多氯联苯。近年来,由于其广泛的底物谱和优异的降解性能,菌株LB400已成为研究原核生物降解多氯联苯的生物化学和分子生物学方面的模式生物。目前关于PCBs的微生物降解研究已不再局限于对微生物资源的挖掘,而是更多地聚焦在LB400等降解菌的PCBs降解基因、降解酶的酶学特性以及酶的人工分子进化等方面。同时,LB400作为早期发现的降解菌,其对多氯联苯的降解途径、底物范围及相关机制也被广泛探讨;但是对于PCBs降解相关基因的调控研究较少。因此,本文以Burkholderia xenovorans LB400对多氯联苯降解为核心,通过综述其代谢途径、代谢相关基因和酶系以及降解应用等方面的研究进展,以期为深入探讨Burkholderia xenovorans LB400的应用以及进一步在遗传、分子和生化水平研究其他多氯联苯降解菌株提供借鉴。
文章类型Review
资助机构中国科学院前沿科学重点研究计划; 中国科学院对外合作计划; 中国科学院青年创新促进会项目
收录类别CSCD
语种中文
CSCD记录号CSCD:6824783
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30327
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.中国科学院烟台海岸带研究所海岸带生物学与生物资源保护实验室,山东烟台264003;
2.青岛海洋科学与技术试点国家实验室,海洋生物学与生物技术功能实验室,山东青岛266237;
3.中国科学院大学,北京100049
推荐引用方式
GB/T 7714孙成成,李俊德,闵军,等. 多氯联苯降解菌Burkholderia xenovorans LB400研究进展[J]. 微生物学通报,2020,47(10):3393-3406.
APA孙成成,李俊德,闵军,&胡晓珂.(2020).多氯联苯降解菌Burkholderia xenovorans LB400研究进展.微生物学通报,47(10),3393-3406.
MLA孙成成,et al."多氯联苯降解菌Burkholderia xenovorans LB400研究进展".微生物学通报 47.10(2020):3393-3406.
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