其他题名Effects of nanoplastics and nanosized titanium dioxide on hemocytes function and antioxidant enzyme activities of Litopenaeus vannamei via dietary exposure
朱效鹏1,2,3; 单恩翠1,2,3; 赵建民1,2
; 王清1,2发表期刊海洋通报
ISSN1001-6392
2021
卷号40期号:3页码:337-347
关键词凡纳滨对虾纳米塑料纳米二氧化钛氧化应激血细胞功能
研究领域Environmental Sciences & Ecology
英文摘要The increasing use of nanoplastics and nanometal oxide particles in different fields have aroused concern about the environmental risks associated with these nanomaterials entering the aquatic system. In the present study, through dietary exposure route, the interference of hemocytes function and oxidative balance of the white shrimp Litopenaeus vannamei after exposure to 100 nm nano-PS and nano-TiO_2 was analyzed. Shrimps of treatment groups were fed with diets spiked with nano- PS and nano-TiO_2 at doses of 200 mg/kg and 2 000 mg/kg respectively for 28 days. The ROS production, phagocytosis and apoptosis rate of hemocytes as well as the enzymatic activities of total antioxidant capacity (T-AOC), superoxidase dismutase (SOD), glutathione-S-transferase (GST), malondialdehyde level (MDA) and Na+/K+-ATPase in hepatopancreas were measured at Day-14 and Day-28. The results showed that both nano-PS and nano-TiO_2 caused a significant increase in the apoptosis rate of hemocytes. Besides, the activities of T-AOC, SOD and GST in hepatopancreas were elevated to varying degrees, while the significant decrease of Na~+/K~+-ATPase activity was observed in the treatment groups. In addition, compared with nano-PS, nano-TiO_2 caused more serious oxidative damage to shrimps after 28 days of exposure.
中文摘要纳米塑料和纳米金属氧化物粒子在不同领域的使用量日益增多,这些纳米材料进入水生系统可能带来的相关环境风险引起了人们的关注。本文通过食相暴露,分析了100 nm纳米聚苯乙烯(PS)和纳米二氧化钛(TiO_2)暴露对凡纳滨对虾(Litopenaeus vannamei)的血细胞功能和氧化还原平衡的干扰。实验组对虾分别投喂混有200 mg/kg和2 000 mg/kg纳米PS和纳米TiO_2的饲料,试验周期为28天。在暴露第14天和第28天测定其对凡纳滨对虾的血细胞ROS含量变化、细胞吞噬率和凋亡率,以及肝胰腺组织内的总抗氧化酶(T-AOC)活性、超氧化物歧化酶(SOD)活性、谷胱甘肽巯基转移酶(GST)活性、丙二醛(MDA)含量和Na+/K+-ATP酶活性的影响。结果表明,纳米PS和纳米TiO_2均导致凡纳滨对虾血细胞凋亡率显著上升,并且导致肝胰腺组织T-AOC、SOD和GST活性不同程度的升高以及Na~+/K~+-ATP酶活性显著降低。生物标志物指数(IBR)结果显示,纳米TiO_2对凡纳滨对虾产生的胁迫压力高于纳米PS。
文章类型Article
资助机构国家自然科学基金
收录类别CSCD
语种中文
CSCD记录号CSCD:7015888
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/30262
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位1.中国科学院烟台海岸带研究所海岸带生物资源高效利用研究与发展中心,山东烟台264003;
2.中国科学院烟台海岸带研究所牟平海岸带环境综合试验站,山东烟台264117;
3.中国科学院大学,北京100049
推荐引用方式
GB/T 7714朱效鹏,单恩翠,赵建民,等. 纳米塑料和二氧化钛食相暴露对凡纳滨对虾血细胞功能及抗氧化系统的影响[J]. 海洋通报,2021,40(3):337-347.
APA朱效鹏,单恩翠,赵建民,&王清.(2021).纳米塑料和二氧化钛食相暴露对凡纳滨对虾血细胞功能及抗氧化系统的影响.海洋通报,40(3),337-347.
MLA朱效鹏,et al."纳米塑料和二氧化钛食相暴露对凡纳滨对虾血细胞功能及抗氧化系统的影响".海洋通报 40.3(2021):337-347.
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