Zhang, Xia1,5; Zhang, Liangwei1,3
; Wang, Xiaoyan1,4; Han, Xiaoyue1,5; Huang, Yan1,4; Li, Bowei1,3; Chen, Lingxin1,2,3发表期刊JOURNAL OF HAZARDOUS MATERIALS
ISSN0304-3894
2021-10-05
卷号419页码:10
关键词Fluorescent probeCysteineNear-infrared imagingHypoxiareperfusionEnvironmental stress
DOI10.1016/j.jhazmat.2021.126476
通讯作者Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Increasingly grim environmental pollutions are closely related with the occurrence and development of diseases. However, it's obscure how environmental stress disturbs the normal physiological process, and then how endogenous reactive species mend the cases. Hypoxia/reperfusion (H/R), a common and intractable injury in aquaculture and clinic, can induce oxidative stress and ultimately cause irreversible injury to organism. Cysteine (Cys) plays essential roles in maintaining transduction of numerous reactive species and redox homeostasis in subcellular structures, cells and organisms. A great deal of fluorescence research about Cys are focusing on development of selective probes but with poor exploration of the biofunction under environmental stress. Therefore, it is of great significance to examine the bio-effects of Cys against H/R stress. In the present work, we design a fluorescent probe BCy-AC for in situ detecting Cys, the unique Enol-Keto tautomerization of fluorophore BCy-Keto propels the reaction process which will improve the sensitivity and potential application performance of the probe. BCy-AC is conveniently applied to visualize Cys in HT-22 cells, zebrafish and mice tissues. Moreover, imaging results obtained from H/R models reveal that endogenous Cys changes with hypoxia and reperfusion time and Cys pretreatment effectively defend H/R injury in cells and in vivo.
资助机构National Natural Science Foundation of China; Science and Technology Innovation Development Plan of Yantai of China; Shandong Provincial Natural Science Founda-tion Key Project; Program of Youth Innovation Promotion Association, CAS; Taishan Scholar Project Special Funding
收录类别SCI
语种英语
关键词[WOS]SELECTIVE DETECTION; GLUTATHIONE DEPLETION; GOLD NANOPARTICLES; REACTIVE OXYGEN; RECENT PROGRESS; PROBE; POLYSULFIDES; LUMINESCENT
研究领域[WOS]Engineering; Environmental Sciences & Ecology
WOS记录号WOS:000694784800001
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29744
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
中科院海岸带环境过程与生态修复重点实验室
通讯作者Chen, Lingxin作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Zhang, Xia,Zhang, Liangwei,Wang, Xiaoyan,et al. Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,419:10.
APAZhang, Xia.,Zhang, Liangwei.,Wang, Xiaoyan.,Han, Xiaoyue.,Huang, Yan.,...&Chen, Lingxin.(2021).Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress.JOURNAL OF HAZARDOUS MATERIALS,419,10.
MLAZhang, Xia,et al."Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress".JOURNAL OF HAZARDOUS MATERIALS 419(2021):10.
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